Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.16 extracted from

  • Bowman, W.H.; White Tabor, C.; Tabor, H.
    Spermidine biosynthesis. Purification and properties of propylamine transferase from Escherichia coli (1973), J. Biol. Chem., 248, 2480-2486.
    View publication on PubMed
Search for more literature for 2.5.1.16

General Stability

General Stability Organism
freeze-thawing inactivates Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
5'-ethylthioadenosine
-
 Escherichia coli
additional information inhibition by end products of reaction; no inhibition by phenylhydrazine, semicarbazide, sodium borohydride, NaCN, KCl, NH4Cl, MgCl2, CaCl2, NaNO3, Na2SO4, Na2HPO4 Escherichia coli
N-ethylmaleimide
-
 Escherichia coli
p-hydroxymercuribenzoate 2-mercaptoethanol restores activity Escherichia coli
spermidine
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0022
-
 S-adenosyl-3-methylthio-1-propylamine pH 8.0, 37ºC, fluorometric assay Escherichia coli
0.012
-
 1,4-diaminobutane pH 8.8, 37ºC, spectrophotometric assay Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70600
-
 W strain, sedimentation equilibrium centrifugation Escherichia coli
73000
-
 W strain Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane Escherichia coli
-
 5'-methylthioadenosine + spermidine
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 W strain
-

Purification (Commentary)

Purification (Comment) Organism
2000fold purified Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.83
-
-
 Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, partially purified enzyme, several weeks with less than 20% loss of activity Escherichia coli
0-4°C, purified enzyme, 10-20% loss of activity within 6 months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Escherichia coli 5'-methylthioadenosine + spermidine
-
 ?
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Escherichia coli 5'-methylthioadenosine + spermidine
-
 ir
S-adenosyl-(5')-3-methylthio-1-propylamine + spermidine at a reduced rate Escherichia coli 5'-methylthioadenosine + spermine the reaction rate is slower than with 1,4-diaminobutane ?
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane
-
 Escherichia coli 5'-methylthioadenosine + spermidine
-
 ?
S-adenosyl-3-methylthio-1-propylamine + 1,5-diaminopentane
-
 Escherichia coli 5'-methylthioadenosine + N-(3-aminopropyl)-1,5-diaminopentane
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 30000-35000, SDS-PAGE Escherichia coli
dimer 2 * 37000-38000, high speed sedimentation equilibrium centrifugation in 6 M guanidine-HCl and 0.1 M 2-mercaptoethanol Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 at least 30 min stable in 0.01 M Tris-HCl buffer, pH 8, 1 mM EDTA Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.4
-
 65% rate of reaction at pH 8.6 and 93% rate of reaction at pH 10.6 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
8.6 10.6 about 65% of maximal activity at pH 8.6 and about 90% of maximal activity at pH 10.6 Escherichia coli