Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-methylthioadenosine | weak inhibition | Bos taurus | |
5'-methylthioadenosine | weak inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
S-adenosyl-(5')-3-methylthio-1-propylamine | pH 7.2, 37ºC | Rattus norvegicus | |
0.01 | - |
S-adenosyl-(5')-3-methylthio-1-propylamine | pH 7.2, 37ºC | Bos taurus | |
0.03 | - |
1,4-diaminobutane | pH 7.2, 37ºC | Bos taurus | |
0.1 | - |
1,4-diaminobutane | pH 7.2, 37ºC | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35800 | - |
2 * 35800, SDS-PAGE | Bos taurus |
37000 | - |
2 * 37000, SDS-PAGE | Rattus norvegicus |
73000 | - |
gel filtration | Rattus norvegicus |
73000 | - |
gel filtration | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | Rattus norvegicus | - |
5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | Bos taurus | - |
5'-methylthioadenosine + spermidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
extraction, ammonium sulfate fractionation, chromatography on DEAE-cellulose, affinity chromatography, gel filtration, 4480fold purified | Rattus norvegicus |
extraction, ammonium sulfate fractionation, chromatography on DEAE-Cellulose, chromatography on hydroxyapatite, chromatography on ATPA-Sepharose, gel filtration | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
brain | - |
Bos taurus | - |
prostate gland | ventral | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.712 | - |
- |
Bos taurus |
1.3 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
0°C, in buffer containing 3 M NaCl, 1 mM DTT and 0.05-0.25 mM decarboxylated S-adenosylmethionine, several months | Rattus norvegicus |
0°C, in buffer containing 3 M NaCl, 1 mM DTT and 0.05-0.25 mM decarboxylated S-adenosylmethionine, several months | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Rattus norvegicus | 5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Bos taurus | 5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,6-diaminohexane | - |
Rattus norvegicus | ? | - |
? | |
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,6-diaminohexane | - |
Bos taurus | ? | - |
? | |
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Rattus norvegicus | 5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Bos taurus | 5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-3-methylthio-1-propylamine + 1,5-diaminopentane | - |
Rattus norvegicus | 5'-methylthioadenosine + N-(3-aminopropyl)-1,5-diaminopentane | - |
? | |
S-adenosyl-3-methylthio-1-propylamine + 1,5-diaminopentane | - |
Bos taurus | 5'-methylthioadenosine + N-(3-aminopropyl)-1,5-diaminopentane | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 37000, SDS-PAGE | Rattus norvegicus |
dimer | 2 * 35800, SDS-PAGE | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
37 | - |
assay at | Bos taurus |