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Literature summary for 2.5.1.16 extracted from

  • Samejima, K.; Raina, A.; Yamanoha, B.; Eloranta, T.
    Purification of putrescine aminopropyltransferase (spermidine synthase) from eukaryotic tissues (1983), Methods Enzymol., 94, 270-276.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5'-methylthioadenosine weak inhibition Bos taurus
5'-methylthioadenosine weak inhibition Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
S-adenosyl-(5')-3-methylthio-1-propylamine pH 7.2, 37ºC Rattus norvegicus
0.01
-
S-adenosyl-(5')-3-methylthio-1-propylamine pH 7.2, 37ºC Bos taurus
0.03
-
1,4-diaminobutane pH 7.2, 37ºC Bos taurus
0.1
-
1,4-diaminobutane pH 7.2, 37ºC Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35800
-
2 * 35800, SDS-PAGE Bos taurus
37000
-
2 * 37000, SDS-PAGE Rattus norvegicus
73000
-
gel filtration Rattus norvegicus
73000
-
gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane Rattus norvegicus
-
5'-methylthioadenosine + spermidine
-
?
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane Bos taurus
-
5'-methylthioadenosine + spermidine
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
extraction, ammonium sulfate fractionation, chromatography on DEAE-cellulose, affinity chromatography, gel filtration, 4480fold purified Rattus norvegicus
extraction, ammonium sulfate fractionation, chromatography on DEAE-Cellulose, chromatography on hydroxyapatite, chromatography on ATPA-Sepharose, gel filtration Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Rattus norvegicus
-
brain
-
Bos taurus
-
prostate gland ventral Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.712
-
-
Bos taurus
1.3
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
0°C, in buffer containing 3 M NaCl, 1 mM DTT and 0.05-0.25 mM decarboxylated S-adenosylmethionine, several months Rattus norvegicus
0°C, in buffer containing 3 M NaCl, 1 mM DTT and 0.05-0.25 mM decarboxylated S-adenosylmethionine, several months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane
-
Rattus norvegicus 5'-methylthioadenosine + spermidine
-
?
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane
-
Bos taurus 5'-methylthioadenosine + spermidine
-
?
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,6-diaminohexane
-
Rattus norvegicus ?
-
?
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,6-diaminohexane
-
Bos taurus ?
-
?
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane
-
Rattus norvegicus 5'-methylthioadenosine + spermidine
-
?
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane
-
Bos taurus 5'-methylthioadenosine + spermidine
-
?
S-adenosyl-3-methylthio-1-propylamine + 1,5-diaminopentane
-
Rattus norvegicus 5'-methylthioadenosine + N-(3-aminopropyl)-1,5-diaminopentane
-
?
S-adenosyl-3-methylthio-1-propylamine + 1,5-diaminopentane
-
Bos taurus 5'-methylthioadenosine + N-(3-aminopropyl)-1,5-diaminopentane
-
?

Subunits

Subunits Comment Organism
dimer 2 * 37000, SDS-PAGE Rattus norvegicus
dimer 2 * 35800, SDS-PAGE Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Rattus norvegicus
37
-
assay at Bos taurus