General Stability | Organism |
---|---|
dialysis against glycine-NaOH buffer, pH 8.8 inactivates | Brassica rapa subsp. pekinensis |
DTT and/or EDTA do not stabilize | Brassica rapa subsp. pekinensis |
heat-inactivated enzyme preparation does not restore the activity | Brassica rapa subsp. pekinensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
81000 | - |
gel filtration | Brassica rapa subsp. pekinensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | Escherichia coli | - |
5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | Brassica rapa subsp. pekinensis | - |
5'-methylthioadenosine + spermidine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica rapa subsp. pekinensis | - |
Chinese cabbage, var Pak Choy | - |
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
streptomycin and ammonium sulfate fractionation, acetone precipitation and chromatography on Sephadex G-100, 160fold purified | Brassica rapa subsp. pekinensis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine | first discovered | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Brassica rapa subsp. pekinensis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.28 | - |
- |
Brassica rapa subsp. pekinensis |
Storage Stability | Organism |
---|---|
-15°C, disrupted protoplasts lose more than 90% of activity within 1 week | Brassica rapa subsp. pekinensis |
-20°C, about 30% loss of activity in the final enzyme preparation within 1 month | Brassica rapa subsp. pekinensis |
-20ºC, stable after the acetone precipitation | Brassica rapa subsp. pekinensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Escherichia coli | 5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-(5')-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Brassica rapa subsp. pekinensis | 5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Escherichia coli | 5'-methylthioadenosine + spermidine | - |
? | |
S-adenosyl-3-methylthio-1-propylamine + 1,4-diaminobutane | - |
Brassica rapa subsp. pekinensis | 5'-methylthioadenosine + spermidine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
37 | - |
assay at | Brassica rapa subsp. pekinensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
at a given pH, the activity of the enzyme in Tris buffer is lower than in phosphate or Gly-NaOH buffers | Brassica rapa subsp. pekinensis |
8.8 | - |
in Gly-NaOH buffer | Brassica rapa subsp. pekinensis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
no activity detected in phosphate buffer | Brassica rapa subsp. pekinensis |
10 | - |
no activity detected in Gly-NaOH buffer | Brassica rapa subsp. pekinensis |