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Literature summary for 2.5.1.153 extracted from

  • Chan, H.C.; Feng, X.; Ko, T.P.; Huang, C.H.; Hu, Y.; Zheng, Y.; Bogue, S.; Nakano, C.; Hoshino, T.; Zhang, L.; Lv, P.; Liu, W.; Crick, D.C.; Liang, P.H.; Wang, A.H.; Oldfield, E.; Guo, R.T.
    Structure and inhibition of tuberculosinol synthase and decaprenyl diphosphate synthase from Mycobacterium tuberculosis (2014), J. Am. Chem. Soc., 136, 2892-2896.
    View publication on PubMed
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Crystallization (Commentary)

Crystallization (Comment) Organism
native enzyme and complexes with inhibitor BPH-629, and mutants Y51F with TPP, mutant Y51F/Y90F with TPP, to 1.8-2.7 A resolution Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
D34A compared to wild-type, mutant produces about 1% (13S)-isotuberculosinol and 60% tuberculosinol Mycobacterium tuberculosis
R38A compared to wild-type, mutant produces about 5% (13S)-isotuberculosinol and 140% tuberculosinol Mycobacterium tuberculosis
Y51F production of both (13S)-isotuberculosinol and tuberculosinol is reduced Mycobacterium tuberculosis
Y51F/Y90F no enzymic activity Mycobacterium tuberculosis
Y90F compared to wild-type, mutant produces about 1% (13S)-isotuberculosinol and 49% tuberculosinol Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WJ61 bifunctional tuberculosinol/iso-tuberculosinol synthase, ECs 3.1.7.8 and 3.1.7.9
-
Mycobacterium tuberculosis ATCC 25618 P9WJ61 bifunctional tuberculosinol/iso-tuberculosinol synthase, ECs 3.1.7.8 and 3.1.7.9
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
tuberculosinyl diphosphate + H2O wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol Mycobacterium tuberculosis tuberculosinol + diphosphate
-
 ?
tuberculosinyl diphosphate + H2O
-
 Mycobacterium tuberculosis tuberculosinol + diphosphate reaction of EC 3.1.7.8. Wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol ?
tuberculosinyl diphosphate + H2O wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol Mycobacterium tuberculosis ATCC 25618 tuberculosinol + diphosphate
-
 ?
tuberculosinyl diphosphate + H2O
-
 Mycobacterium tuberculosis ATCC 25618 tuberculosinol + diphosphate reaction of EC 3.1.7.8. Wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol ?
tuberculosinyl diphosphate + H2O
-
 Mycobacterium tuberculosis (13S)-isotuberculosinol + diphosphate reaction of EC 3.1.7.9. Wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol ?
tuberculosinyl diphosphate + H2O
-
 Mycobacterium tuberculosis (13S)-isotuberculosinol + diphosphate wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol ?
tuberculosinyl diphosphate + H2O
-
 Mycobacterium tuberculosis ATCC 25618 (13S)-isotuberculosinol + diphosphate reaction of EC 3.1.7.9. Wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol ?
tuberculosinyl diphosphate + H2O
-
 Mycobacterium tuberculosis ATCC 25618 (13S)-isotuberculosinol + diphosphate wild-type synthesizes 41% (13S)-isotuberculosinol and 59% tuberculosinol ?

Synonyms

Synonyms Comment Organism
Rv3378c
-
 Mycobacterium tuberculosis