Crystallization (Comment) | Organism |
---|---|
apo-enzyme, and enzyme in complex with HPPK substrate 6-hydroxymethyl-7,8-dihydropterin or inhibitor 2-(7-amino-1-methyl-4,5-dioxo-1,4,5,6-tetrahydorpyrimido[4,5-c]pyridazin-3-yl)propanoic acid, X-ray diffraction structure determination and analysis at 2.2-2.3 A resolution | Francisella tularensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-(7-amino-1-methyl-4,5-dioxo-1,4,5,6-tetrahydropyrimido[4,5-c]pyridazin-3-yl)propanoate | binding structure, interactions with the DHPS module and the HPPK module, modeling, ovverview | Francisella tularensis | |
additional information | simultaneously targeting of the two modules of the bifunctional enzyme with pterin binding inhibitors | Francisella tularensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50509 | - |
1 * 50509, sequence calculation | Francisella tularensis |
53000 | - |
gel filtration, analytical sedimentation centrifugation | Francisella tularensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate | Francisella tularensis | - |
diphosphate + 7,8-dihydropteroate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Francisella tularensis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2-amino-4-hydroxy-7,8-dihydropteridin-6-yl)methyl diphosphate + 4-aminobenzoate | - |
Francisella tularensis | diphosphate + 7,8-dihydropteroate | - |
? | |
additional information | the enzyme is bifunctional exhibiting 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, HPPK, and dihydropteroate synthase, DHPS, activities | Francisella tularensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50509, sequence calculation | Francisella tularensis |
More | primary and secondary structures of the HPPK-DHPS bifunctional enzyme, structure comparisons, three-dimensional structure, overview | Francisella tularensis |
Synonyms | Comment | Organism |
---|---|---|
DHPS | - |
Francisella tularensis |
General Information | Comment | Organism |
---|---|---|
additional information | the enzyme is bifunctional exhibiting 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, HPPK, EC 2.7.6.3, and dihydropteroate synthase, DHPS, activities, that catalyze sequential metabolic reactions in the folate biosynthetic pathway of bacteria and lower eukaryotes, structural organization between FtHPPK and FtDHPS which are tethered together by a short linker, overview. Each active site binds substrate in the same manner observed in the monofunctional forms. Structures of the active site loops in the DHPS module | Francisella tularensis |