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Literature summary for 2.5.1.15 extracted from

  • Storozhenko, S.; Navarrete, O.; Ravanel, S.; De Brouwer, V.; Chaerle, P.; Zhang, G.F.; Bastien, O.; Lambert, W.; Rebeille, F.; Van Der Straeten, D.
    Cytosolic hydroxymethyldihydropterin pyrophosphokinase/dihydropteroate synthase from Arabidopsis thaliana: a specific role in early development and stress response (2007), J. Biol. Chem., 282, 10749-10761.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as N-terminal His6 fusion Arabidopsis thaliana
expression in Saccharomyces cerevisiae Fol1 knockout Y26466 Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information bifunctional enzyme, mitochondrial enzyme ubiquitously expressed, cytosolic enzyme only in reproductive tisse, construction of a chimeric protein containing the first 224 amino acids of cytosolic DHPS fused to GFP, expression in Arabidopsis thaliana, Arabidopsis thaliana mutant lacking DHPS indicates a role of DHPS in seed stress Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Arabidopsis thaliana 5829
-
mitochondrion
-
Arabidopsis thaliana 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate Arabidopsis thaliana
-
diphosphate + 7,8-dihydropteroate
-
?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q1ENB6
-
-

Purification (Commentary)

Purification (Comment) Organism
overexpression of the N-terminal His6 fusion protein in Escherichia coli, coexpression with chaperone genes to increase solubility Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-amino-4-hydroxy-6-hydroxymethyl-7,8-dihydropteridine diphosphate + 4-aminobenzoate
-
Arabidopsis thaliana diphosphate + 7,8-dihydropteroate
-
?

Synonyms

Synonyms Comment Organism
7,8-dihydropteroate synthase
-
Arabidopsis thaliana
DHPS
-
Arabidopsis thaliana
DHPS/DHPR
-
Arabidopsis thaliana
dihydropteroate synthase
-
Arabidopsis thaliana