Any feedback?
Literature summary for 2.5.1.144 extracted from
- Enzymatic proof for the identity of the S-sulfocysteine synthase and cysteine synthase B of Salmonella typhimurium (1984), J. Bacteriol., 158, 1122-1127 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-cycloserine | 18% residual activity at 5 mM | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| hydroxylamin | 3% residual activity at 10 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
| additional information | not inhibited by L-methionine | Salmonella enterica subsp. enterica serovar Typhimurium | |
| S-sulfocysteine | 74% residual activity at 5 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
| Semicarbazide | 40% residual activity at 1 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.7 | - |
thiosulfate | at pH 7.6, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 9 | - |
O-acetyl-L-serine | at pH 7.6, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| O-acetyl-L-serine + thiosulfate | Salmonella enterica subsp. enterica serovar Typhimurium | - |
S-sulfo-L-cysteine + acetate | - |
? | |
| O-acetyl-L-serine + thiosulfate | Salmonella enterica subsp. enterica serovar Typhimurium LT-2 | - |
S-sulfo-L-cysteine + acetate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | P29848 | - |
- |
| Salmonella enterica subsp. enterica serovar Typhimurium LT-2 | P29848 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-cellulose DE-32 column chromatography, DEAE-Sepharose CL-6B column chromatography, DEAE-cellulose DE-53 column chromatography, hydroxylapatite column chromatography, and Sephacryl S-200 gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.13 | - |
crude extract, at pH 7.6, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium |
| 276.6 | - |
after 2212fold purification, at pH 7.6, temperature not specified in the publication | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| azaserine + Na2S | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| azaserine + Na2S | - |
Salmonella enterica subsp. enterica serovar Typhimurium LT-2 | ? | - |
? | |
| azaserine + thiosulfate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| azaserine + thiosulfate | - |
Salmonella enterica subsp. enterica serovar Typhimurium LT-2 | ? | - |
? | |
| additional information | no activity with O-phosphoserine, O-succinylhomoserine, and L-serine | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| additional information | no activity with O-phosphoserine, O-succinylhomoserine, and L-serine | Salmonella enterica subsp. enterica serovar Typhimurium LT-2 | ? | - |
? | |
| O-acetyl-L-serine + Na2S | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| O-acetyl-L-serine + Na2S | - |
Salmonella enterica subsp. enterica serovar Typhimurium LT-2 | ? | - |
? | |
| O-acetyl-L-serine + thiosulfate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | S-sulfo-L-cysteine + acetate | - |
? | |
| O-acetyl-L-serine + thiosulfate | - |
Salmonella enterica subsp. enterica serovar Typhimurium LT-2 | S-sulfo-L-cysteine + acetate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cysteine synthase B | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | 77 | the enzyme is inactivated by the treatment around 65 to 67.5°C, and practically complete inactivation is observed at 77.5°C | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| pyridoxal 5'-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
