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Literature summary for 2.5.1.144 extracted from

  • Hensel, G.; Trueper, H.
    O-Acetylserine sulfhydrylase and S-sulfocysteine synthase activities of Rhodospirillum tenue (1983), Arch. Microbiol., 134, 227-232 .
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
additional information methionine, glutathione, homocysteine, and acetate have no influences on the enzyme activity Rhodocyclus tenuis
Sulfide
-
Rhodocyclus tenuis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
Sulfide pH and temperature not specified in the publication Rhodocyclus tenuis
1.9
-
thiosulfate pH and temperature not specified in the publication Rhodocyclus tenuis
9
-
O-acetyl-L-serine with thiosulfate as cosubstrate, pH and temperature not specified in the publication Rhodocyclus tenuis
50
-
O-acetyl-L-serine with sulfide as cosubstrate, pH and temperature not specified in the publication Rhodocyclus tenuis

Organism

Organism UniProt Comment Textmining
Rhodocyclus tenuis
-
-
-
Rhodocyclus tenuis BN230
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, DE52 column chromatography, hydroxyapatite column chromatography, and Sephadex G 100 gel filtration Rhodocyclus tenuis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
O-acetyl-L-serine + sulfide the activity of the enzyme with O-acetylserine plus sulfide is about 1.5 of that with O-acetylserine plus thiosulfate Rhodocyclus tenuis 2-amino-3-sulfanylpropanoic acid + acetate
-
?
O-acetyl-L-serine + sulfide the activity of the enzyme with O-acetylserine plus sulfide is about 1.5 of that with O-acetylserine plus thiosulfate Rhodocyclus tenuis BN230 2-amino-3-sulfanylpropanoic acid + acetate
-
?
O-acetyl-L-serine + thiosulfate the activity of the enzyme with O-acetylserine plus sulfide is about 1.5 of that with O-acetylserine plus thiosulfate Rhodocyclus tenuis S-sulfo-L-cysteine + acetate
-
?
O-acetyl-L-serine + thiosulfate the activity of the enzyme with O-acetylserine plus sulfide is about 1.5 of that with O-acetylserine plus thiosulfate Rhodocyclus tenuis BN230 S-sulfo-L-cysteine + acetate
-
?

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE Rhodocyclus tenuis

Synonyms

Synonyms Comment Organism
S-sulfocysteine synthase
-
Rhodocyclus tenuis
SSC synthase
-
Rhodocyclus tenuis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Rhodocyclus tenuis