Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae | Humulus lupulus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutation of the Asp in Asp-rich motifs in PT1L to Ala and coexpression with wild-type prenyltransferase, the mutant strain does not produce any prenylated products. A truncated variant lacking 83 amino acid from the start codon, displays much higher activity than wild-type | Humulus lupulus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Humulus lupulus | 9507 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Humulus lupulus | A0A0B4ZTQ2 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 2-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one | - |
Humulus lupulus | diphosphate + ? | - |
? | |
dimethylallyl diphosphate + 3-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one | - |
Humulus lupulus | diphosphate + ? | - |
? | |
dimethylallyl diphosphate + a 2-acyl-4,6-bisprenylphloroglucinol | - |
Humulus lupulus | diphosphate + a 2-acyl-4,6,6-trisprenylcyclohexa-2,4-dien-1-one | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aromatic prenyltransferase | - |
Humulus lupulus |
PT2 | - |
Humulus lupulus |
General Information | Comment | Organism |
---|---|---|
physiological function | prenyltransferase PT2 catalyzes the last two prenylation steps in the beta-bitter acid pathway. Coexpression of codon-optimized prenyltransferases PT1L and PT2 in yeast leads to the production of bitter acids. PT1L/PT2 forms and functions as a complex in membranes. PT1L catalyzes the first prenylation step, and PT2 catalyzes the two subsequent prenylation steps | Humulus lupulus |