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Literature summary for 2.5.1.136 extracted from

  • Zhou, K.; Wunsch, C.; Dai, J.; Li, S.M.
    gem-Diprenylation of acylphloroglucinols by a fungal prenyltransferase of the dimethylallyltryptophan synthase superfamily (2017), Org. Lett., 19, 388-391 .
    View publication on PubMed
Search for more literature for 2.5.1.136

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15 [pREP] cells Aspergillus terreus

Inhibitors

Inhibitors Comment Organism Structure
Co2+ strong inhibition at 5 mM Aspergillus terreus
Cu2+ complete inhibition at 5 mM Aspergillus terreus
Fe2+ strong inhibition at 5 mM Aspergillus terreus
Mn2+ complete inhibition at 5 mM Aspergillus terreus
Ni2+ complete inhibition at 5 mM Aspergillus terreus
Zn2+ almost complete inhibition at 5 mM Aspergillus terreus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.071
-
 dimethylallyl diphosphate with 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
0.16
-
 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one with dimethylallyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
0.38
-
 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one with farnesyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
0.42
-
 3-methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one with farnesyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
0.46
-
 geranyl diphosphate with phenyl(2,4,6-trihydroxyphenyl)methanone as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
0.47
-
 phenyl(2,4,6-trihydroxyphenyl)methanone with geranyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
0.7
-
 phenyl(2,4,6-trihydroxyphenyl)methanone with farnesyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulates activity, 5 mM used in assay conditions Aspergillus terreus
Mg2+ stimulates activity Aspergillus terreus
additional information not influenced by K+ and Na+ Aspergillus terreus

Organism

Organism UniProt Comment Textmining
Aspergillus terreus A0A1B0UHJ4
-
-
Aspergillus terreus DSM 1958 A0A1B0UHJ4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one
-
 Aspergillus terreus diphosphate + 2-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one
-
 ?
dimethylallyl diphosphate + 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one
-
 Aspergillus terreus DSM 1958 diphosphate + 2-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one
-
 ?
dimethylallyl diphosphate + 2-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one
-
 Aspergillus terreus diphosphate + 3,5-dihydroxy-4,4-bis(3-methylbut-2-en-1-yl)-2-(2-methylpropanoyl)cyclohexa-2,5-dien-1-one
-
 ?
dimethylallyl diphosphate + 2-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one
-
 Aspergillus terreus DSM 1958 diphosphate + 3,5-dihydroxy-4,4-bis(3-methylbut-2-en-1-yl)-2-(2-methylpropanoyl)cyclohexa-2,5-dien-1-one
-
 ?
dimethylallyl diphosphate + 3-methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one
-
 Aspergillus terreus diphosphate + 3-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]butan-1-one
-
 ?
dimethylallyl diphosphate + 3-methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one
-
 Aspergillus terreus DSM 1958 diphosphate + 3-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]butan-1-one
-
 ?
dimethylallyl diphosphate + 3-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]butan-1-one
-
 Aspergillus terreus diphosphate + 3,5-dihydroxy-2-(3-methylbutanoyl)-4,4-bis(3-methylbut-2-en-1-yl)cyclohexa-2,5-dien-1-one
-
 ?
dimethylallyl diphosphate + 3-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]butan-1-one
-
 Aspergillus terreus DSM 1958 diphosphate + 3,5-dihydroxy-2-(3-methylbutanoyl)-4,4-bis(3-methylbut-2-en-1-yl)cyclohexa-2,5-dien-1-one
-
 ?
dimethylallyl diphosphate + phenyl(2,4,6-trihydroxyphenyl)methanone
-
 Aspergillus terreus diphosphate + phenyl[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]methanone
-
 ?
dimethylallyl diphosphate + phenyl(2,4,6-trihydroxyphenyl)methanone
-
 Aspergillus terreus DSM 1958 diphosphate + phenyl[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]methanone
-
 ?
dimethylallyl diphosphate + phenyl[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]methanone
-
 Aspergillus terreus diphosphate + 2-benzoyl-3,5-dihydroxy-4,4-bis(3-methylbut-2-en-1-yl)cyclohexa-2,5-dien-1-one
-
 ?
farnesyl diphosphate + 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one
-
 Aspergillus terreus diphosphate + 2-methyl-1-[2,4,6-trihydroxy-3-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]phenyl]propan-1-one
-
 ?
farnesyl diphosphate + 3-methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one
-
 Aspergillus terreus diphosphate + 3-methyl-1-[2,4,6-trihydroxy-3-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]phenyl]butan-1-one
-
 ?
farnesyl diphosphate + phenyl(2,4,6-trihydroxyphenyl)methanone
-
 Aspergillus terreus diphosphate + phenyl[2,4,6-trihydroxy-3-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]phenyl]methanone
-
 ?
geranyl diphosphate + 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one
-
 Aspergillus terreus diphosphate + 1-[3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2,4,6-trihydroxyphenyl]-2-methylpropan-1-one
-
 ?
geranyl diphosphate + 3-methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one
-
 Aspergillus terreus diphosphate + 1-[3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2,4,6-trihydroxyphenyl]-3-methylbutan-1-one
-
 ?
geranyl diphosphate + phenyl(2,4,6-trihydroxyphenyl)methanone
-
 Aspergillus terreus diphosphate + [3-[(2E)-3,7-dimethylocta-2,6-dien-1-yl]-2,4,6-trihydroxyphenyl](phenyl)methanone
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Aspergillus terreus

Synonyms

Synonyms Comment Organism
apT
-
 Aspergillus terreus
aromatic prenyltransferase
-
 Aspergillus terreus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.012
-
 3-methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one with farnesyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
0.012
-
 phenyl(2,4,6-trihydroxyphenyl)methanone with farnesyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
0.014
-
 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one with farnesyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
0.126
-
 geranyl diphosphate with 3 as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
0.126
-
 geranyl diphosphate with phenyl(2,4,6-trihydroxyphenyl)methanone as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
0.137
-
 phenyl(2,4,6-trihydroxyphenyl)methanone with geranyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
0.27
-
 dimethylallyl diphosphate with 1 as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
0.27
-
 dimethylallyl diphosphate with 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
0.284
-
 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one with dimethylallyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0171
-
 phenyl(2,4,6-trihydroxyphenyl)methanone with farnesyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
0.0286
-
 3-methyl-1-(2,4,6-trihydroxyphenyl)butan-1-one with farnesyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
0.0368
-
 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one with farnesyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
0.2739
-
 geranyl diphosphate with phenyl(2,4,6-trihydroxyphenyl)methanone as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
0.2915
-
 phenyl(2,4,6-trihydroxyphenyl)methanone with geranyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
1.775
-
 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one with dimethylallyl diphosphate as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus
3.8169
-
 dimethylallyl diphosphate with 2-methyl-1-(2,4,6-trihydroxyphenyl)propan-1-one as cosubstrate, at pH 7.5 and 37°C Aspergillus terreus