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Literature summary for 2.5.1.136 extracted from

  • Li, H.; Ban, Z.; Qin, H.; Ma, L.; King, A.J.; Wang, G.
    A heteromeric membrane-bound prenyltransferase complex from hop catalyzes three sequential aromatic prenylations in the bitter acid pathway (2015), Plant Physiol., 167, 650-659.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.136

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae Humulus lupulus

Protein Variants

Protein Variants Comment Organism
additional information mutation of the Asp in Asp-rich motifs in PT1L to Ala and coexpression with wild-type prenyltransferase, the mutant strain does not produce any prenylated products. A truncated variant lacking 83 amino acid from the start codon, displays much higher activity than wild-type Humulus lupulus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00136
-
 phlorisovalerophenone truncated recombinant protein, pH 7.0, 30°C Humulus lupulus
0.00339
-
 phlorisovalerophenone presence of prenyltransferase PT2, pH 7.0, 30°C Humulus lupulus
0.0051
-
 phlorisobutyrophenone truncated recombinant protein, pH 7.0, 30°C Humulus lupulus
0.0057
-
 geranyl diphosphate truncated recombinant protein, pH 7.0, 30°C Humulus lupulus
0.00618
-
 geranyl diphosphate presence of prenyltransferase PT2, pH 7.0, 30°C Humulus lupulus
0.00668
-
 phlorisobutyrophenone presence of prenyltransferase PT2, pH 7.0, 30°C Humulus lupulus
0.0435
-
 dimethylallyl diphosphate truncated recombinant protein, pH 7.0, 30°C Humulus lupulus
0.0436
-
 dimethylallyl diphosphate presence of prenyltransferase PT2, pH 7.0, 30°C Humulus lupulus

Organism

Organism UniProt Comment Textmining
Humulus lupulus A0A0B5A051
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + phlorisobutyrophenone
-
 Humulus lupulus diphosphate + 2-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one
-
 ?
dimethylallyl diphosphate + phlorisovalerophenone
-
 Humulus lupulus diphosphate + 3-methyl-1-[2,4,6-trihydroxy-3-(3-methylbut-2-en-1-yl)phenyl]propan-1-one
-
 ?
geranyl diphosphate + phlorisovalerophenone
-
 Humulus lupulus diphosphate + 3-methyl-1-[2,4,6-trihydroxy-3-geranylphenyl]propan-1-one
-
 ?

Synonyms

Synonyms Comment Organism
aromatic prenyltransferase
-
 Humulus lupulus
PT1L
-
 Humulus lupulus

General Information

General Information Comment Organism
physiological function coexpression of codon-optimized prenyltransferases PT1L and PT2 in yeast leads to the production of bitter acids. PT1L/PT2 forms and functions as a complex in membranes. PT1L catalyzes the first prenylation step, and PT2 catalyzes the two subsequent prenylation steps Humulus lupulus