Literature summary for 2.5.1.132 extracted from

Wang, L.; Lu, Z.; Allen, K.; Mariano, P.; Dunaway-Mariano, D.
Human symbiont Bacteroides thetaiotaomicron synthesizes 2-keto-3-deoxy-D-glycero-D-galacto-nononic acid (KDN) (2008), Chem. Biol., 15, 893-897.

Search for more literature for 2.5.1.132

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.11	-	phosphoenolpyruvate	pH 7.5, 25°C	Bacteroides thetaiotaomicron
1.4	-	D-mannose 6-phosphate	pH 7.5, 25°C	Bacteroides thetaiotaomicron

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
phosphoenolpyruvate + D-mannose 6-phosphate + H2O	Bacteroides thetaiotaomicron	the 3-deoxy-D-glycero-D-galacto-nononate unit might be used to protect the cell from bacteriophage invasion and/or to mimic the human epithelial cell surface	3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Bacteroides thetaiotaomicron	Q8A711	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphoenolpyruvate + D-mannose 6-phosphate + H2O	the 3-deoxy-D-glycero-D-galacto-nononate unit might be used to protect the cell from bacteriophage invasion and/or to mimic the human epithelial cell surface	Bacteroides thetaiotaomicron	3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + phosphate	-	r
phosphoenolpyruvate + D-mannose 6-phosphate + H2O	the enzyme is specific for mannose 6-phosphate	Bacteroides thetaiotaomicron	3-deoxy-D-glycero-D-galacto-nononate 9-phosphate + phosphate	-	r

Synonyms

Synonyms	Comment	Organism
BT1714	-	Bacteroides thetaiotaomicron
KDN-9P synthase	-	Bacteroides thetaiotaomicron

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Bacteroides thetaiotaomicron

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.017	-	phosphoenolpyruvate	pH 7.5, 25°C	Bacteroides thetaiotaomicron
0.017	-	D-mannose 6-phosphate	pH 7.5, 25°C	Bacteroides thetaiotaomicron

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Bacteroides thetaiotaomicron

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.012	-	D-mannose 6-phosphate	pH 7.5, 25°C	Bacteroides thetaiotaomicron
0.15	-	phosphoenolpyruvate	pH 7.5, 25°C	Bacteroides thetaiotaomicron

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Release 2023.1 (January 2023)