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Literature summary for 2.5.1.127 extracted from

  • Knott, J.M.
    Biosynthesis of long-chain polyamines by crenarchaeal polyamine synthases from Hyperthermus butylicus and Pyrobaculum aerophilum (2009), FEBS Lett., 583, 3519-3524.
    View publication on PubMed
Search for more literature for 2.5.1.127

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli with an N-terminal 6* His sequence Hyperthermus butylicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32400
-
 x * 32400, calculation from sequence Hyperthermus butylicus
35000
-
 x * 35000, SDS-PAGE Hyperthermus butylicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl 3-(methylthio)propylamine + caldopentamine Hyperthermus butylicus the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + caldoheptamine
-
 ?
2 S-adenosyl 3-(methylthio)propylamine + caldopentamine Hyperthermus butylicus DSM 5456 the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + caldoheptamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + caldopentamine Hyperthermus butylicus the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + caldohexamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + caldopentamine Hyperthermus butylicus DSM 5456 the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + caldohexamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + norspermidine Hyperthermus butylicus the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + norspermine
-
 ?
S-adenosyl 3-(methylthio)propylamine + norspermidine Hyperthermus butylicus DSM 5456 the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + norspermine
-
 ?
S-adenosyl 3-(methylthio)propylamine + norspermine Hyperthermus butylicus the long-chain polyamines stabilize double-stranded DNA at high temperatures S-methyl-5'-thioadenosine + caldopentamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + spermidine Hyperthermus butylicus spermidine is not the most preferred substrate for any enzyme. Nevertheless, in vivo the enzyme can be responsible for the synthesis of thermospermine, if spermidine is present in a high concentration in competition to other accepted polyamines S-methyl-5'-thioadenosine + thermospermine + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Hyperthermus butylicus A2BIX4
-
-
Hyperthermus butylicus DSM 5456 A2BIX4
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Hyperthermus butylicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.002
-
 pH 7.5, 95°C, substrate: propane-1,3-diamine Hyperthermus butylicus
0.018
-
 pH 7.5, 95°C, substrate: spermidine Hyperthermus butylicus
0.064
-
 pH 7.5, 95°C, substrate: norspermidine Hyperthermus butylicus
0.112
-
 pH 7.5, 95°C, substrate: norspermine Hyperthermus butylicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl 3-(methylthio)propylamine + caldopentamine
-
 Hyperthermus butylicus S-methyl-5'-thioadenosine + caldoheptamine
-
 ?
2 S-adenosyl 3-(methylthio)propylamine + caldopentamine the long-chain polyamines stabilize double-stranded DNA at high temperatures Hyperthermus butylicus S-methyl-5'-thioadenosine + caldoheptamine
-
 ?
2 S-adenosyl 3-(methylthio)propylamine + caldopentamine
-
 Hyperthermus butylicus DSM 5456 S-methyl-5'-thioadenosine + caldoheptamine
-
 ?
2 S-adenosyl 3-(methylthio)propylamine + caldopentamine the long-chain polyamines stabilize double-stranded DNA at high temperatures Hyperthermus butylicus DSM 5456 S-methyl-5'-thioadenosine + caldoheptamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + caldopentamine
-
 Hyperthermus butylicus S-methyl-5'-thioadenosine + caldohexamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + caldopentamine the long-chain polyamines stabilize double-stranded DNA at high temperatures Hyperthermus butylicus S-methyl-5'-thioadenosine + caldohexamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + caldopentamine
-
 Hyperthermus butylicus DSM 5456 S-methyl-5'-thioadenosine + caldohexamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + caldopentamine the long-chain polyamines stabilize double-stranded DNA at high temperatures Hyperthermus butylicus DSM 5456 S-methyl-5'-thioadenosine + caldohexamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + norspermidine the long-chain polyamines stabilize double-stranded DNA at high temperatures Hyperthermus butylicus S-methyl-5'-thioadenosine + norspermine
-
 ?
S-adenosyl 3-(methylthio)propylamine + norspermidine at 57% of the specific activity compared to norspermine Hyperthermus butylicus S-methyl-5'-thioadenosine + norspermine
-
 ?
S-adenosyl 3-(methylthio)propylamine + norspermidine the long-chain polyamines stabilize double-stranded DNA at high temperatures Hyperthermus butylicus DSM 5456 S-methyl-5'-thioadenosine + norspermine
-
 ?
S-adenosyl 3-(methylthio)propylamine + norspermine
-
 Hyperthermus butylicus S-methyl-5'-thioadenosine + caldopentamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + norspermine the long-chain polyamines stabilize double-stranded DNA at high temperatures Hyperthermus butylicus S-methyl-5'-thioadenosine + caldopentamine
-
 ?
S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine at 2% of the specific activity compared to norspermine Hyperthermus butylicus S-methyl-5'-thioadenosine + norspermidine
-
 ?
S-adenosyl 3-(methylthio)propylamine + spermidine spermidine is not the most preferred substrate for any enzyme. Nevertheless, in vivo the enzyme can be responsible for the synthesis of thermospermine, if spermidine is present in a high concentration in competition to other accepted polyamines Hyperthermus butylicus S-methyl-5'-thioadenosine + thermospermine + H+
-
 ?
S-adenosyl 3-(methylthio)propylamine + spermidine at 16% of the specific activity compared to norspermine Hyperthermus butylicus S-methyl-5'-thioadenosine + thermospermine + H+
-
 ?

Subunits

Subunits Comment Organism
? x * 35000, SDS-PAGE Hyperthermus butylicus
? x * 32400, calculation from sequence Hyperthermus butylicus

Synonyms

Synonyms Comment Organism
HbSpe II
-
 Hyperthermus butylicus
long-chain polyamine synthase ambiguous Hyperthermus butylicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
 assay at Hyperthermus butylicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Hyperthermus butylicus