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Literature summary for 2.5.1.122 extracted from

  • Fan, A.; Xie, X.; Li, S.M.
    Tryptophan prenyltransferases showing higher catalytic activities for Friedel-Crafts alkylation of o- and m-tyrosines than tyrosine prenyltransferases (2015), Org. Biomol. Chem., 13, 7551-7557.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.58
-
L-o-tyrosine pH 7.5, 37°C, recombinant enzyme Aspergillus niger

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dimethylallyl diphosphate + L-tyrosine Aspergillus niger O-4 prenylation diphosphate + 4-O-dimethylallyl-L-tyrosine
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dimethylallyl diphosphate + L-o-tyrosine C-5 prenylation Aspergillus niger diphosphate + 5-(3-methylbut-2-enyl)-L-o-tyrosine
-
?
dimethylallyl diphosphate + L-tryptophan C-7 prenylation Aspergillus niger diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
-
?
dimethylallyl diphosphate + L-tyrosine O-4 prenylation Aspergillus niger diphosphate + 4-O-dimethylallyl-L-tyrosine
-
?
additional information HR-ESI-MS and NMR product analysis. No activity with L-m-tyrosine Aspergillus niger ?
-
?

Synonyms

Synonyms Comment Organism
TyrPT
-
Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Aspergillus niger

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.014
-
L-o-tyrosine pH 7.5, 37°C, recombinant enzyme Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Aspergillus niger

General Information

General Information Comment Organism
evolution the enzyme is part of the DMATS superfamily Aspergillus niger

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0241
-
L-o-tyrosine pH 7.5, 37°C, recombinant enzyme Aspergillus niger