KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
methylallyl diphosphate | cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37°C | Aspergillus niger | |
0.13 | - |
methylallyl diphosphate | cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37°C | Leptosphaeria maculans | |
0.17 | - |
dimethylallyl diphosphate | pH 7.5, 37°C | Leptosphaeria maculans | |
0.22 | - |
2-pentenyl diphosphate | cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37°C | Leptosphaeria maculans | |
0.23 | - |
2-pentenyl diphosphate | cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37°C | Aspergillus niger | |
0.52 | - |
benzyl diphosphate | cosubstrate 4-aminophenylalanine, pH 7.5, 37°C | Aspergillus niger | |
0.71 | - |
dimethylallyl diphosphate | pH 7.5, 37°C | Aspergillus niger | |
0.75 | - |
benzyl diphosphate | cosubstrate 4-aminophenylalanine, pH 7.5, 37°C | Leptosphaeria maculans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Leptosphaeria maculans | Q6Q874 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-pentenyl diphosphate + 3-fluoro-DL-tyrosine | - |
Leptosphaeria maculans | diphosphate + 3-fluoro-4-O-(2-pentenyl)-DL-tyrosine | 20.5% conversion | ? | |
2-pentenyl diphosphate + 3-fluoro-DL-tyrosine | - |
Aspergillus niger | diphosphate + 3-fluoro-4-O-(2-pentenyl)-DL-tyrosine | 43.9% conversion | ? | |
2-pentenyl diphosphate + 4-amino-L-phenylalanine | - |
Leptosphaeria maculans | diphosphate + 4-(2-pentenylamino)-L-phenylalanine | 42.6% conversion | ? | |
2-pentenyl diphosphate + 4-amino-L-phenylalanine | - |
Aspergillus niger | diphosphate + 4-(2-pentenylamino)-L-phenylalanine | 46.4% conversion | ? | |
2-pentenyl diphosphate + alpha-methyl-L-tyrosine | - |
Aspergillus niger | diphosphate + alpha-methyl-4-O-(2-pentenyl)-L-tyrosine | 10.3% conversion | ? | |
2-pentenyl diphosphate + alpha-methyl-L-tyrosine | - |
Leptosphaeria maculans | diphosphate + alpha-methyl-4-O-(2-pentenyl)-L-tyrosine | 33.5% conversion | ? | |
2-pentenyl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | diphosphate + 4-O-(2-pentenyl)-L-tyrosine | 44.3% conversion | ? | |
2-pentenyl diphosphate + L-tyrosine | - |
Aspergillus niger | diphosphate + 4-O-(2-pentenyl)-L-tyrosine | 56.6% conversion | ? | |
benzyl diphosphate + 4-amino-L-phenylalanine | - |
Leptosphaeria maculans | diphosphate + 4-(benzylamino)-L-phenylalanine | 54.6% conversion | ? | |
benzyl diphosphate + 4-amino-L-phenylalanine | - |
Aspergillus niger | diphosphate + 4-(benzylamino)-L-phenylalanine | 67.2% conversion | ? | |
dimethylallyl diphosphate + 3,4-dihydroxy-L-phenylalanine | - |
Aspergillus niger | diphosphate + 3-hydroxy-4-dimethylallyloxy-L-phenylalanine | 100% conversion | ? | |
dimethylallyl diphosphate + 3,4-dihydroxy-L-phenylalanine | - |
Leptosphaeria maculans | diphosphate + 3-hydroxy-4-dimethylallyloxy-L-phenylalanine | 88.2% conversion | ? | |
dimethylallyl diphosphate + 3,5-dibromo-4-hydroxy-L-phenylalanine | - |
Aspergillus niger | diphosphate + 3,5-dibromo-4-dimethylallyloxy-L-phenylalanine | 20.6% conversion | ? | |
dimethylallyl diphosphate + 3,5-dibromo-4-hydroxy-L-phenylalanine | - |
Leptosphaeria maculans | diphosphate + 3,5-dibromo-4-dimethylallyloxy-L-phenylalanine | 53.3% conversion | ? | |
dimethylallyl diphosphate + 3-fluoro-DL-tyrosine | - |
Aspergillus niger | diphosphate + 3-fluoro-4-O-dimethylallyl-DL-tyrosine | 50.1% conversion | ? | |
dimethylallyl diphosphate + 3-fluoro-DL-tyrosine | - |
Leptosphaeria maculans | diphosphate + 3-fluoro-4-O-dimethylallyl-DL-tyrosine | 66.5% conversion | ? | |
dimethylallyl diphosphate + 3-iodo-L-tyrosine | - |
Aspergillus niger | diphosphate + 3-iodo-4-O-dimethylallyl-L-tyrosine | 100% conversion | ? | |
dimethylallyl diphosphate + 3-iodo-L-tyrosine | - |
Leptosphaeria maculans | diphosphate + 3-iodo-4-O-dimethylallyl-L-tyrosine | 100% conversion | ? | |
dimethylallyl diphosphate + 4-amino-L-phenylalanine | - |
Leptosphaeria maculans | diphosphate + 4-(dimethylallylamino)-L-phenylalanine | 66.1% conversion | ? | |
dimethylallyl diphosphate + 4-amino-L-phenylalanine | - |
Aspergillus niger | diphosphate + 4-(dimethylallylamino)-L-phenylalanine | 78.1% conversion | ? | |
dimethylallyl diphosphate + alpha-methyl-L-tyrosine | - |
Leptosphaeria maculans | diphosphate + alpha-methyl-4-O-dimethylallyl-L-tyrosine | 89.4% conversion | ? | |
dimethylallyl diphosphate + alpha-methyl-L-tyrosine | - |
Aspergillus niger | diphosphate + alpha-methyl-4-O-dimethylallyl-L-tyrosine | 98.3% conversion | ? | |
dimethylallyl diphosphate + D-tyrosine | - |
Leptosphaeria maculans | diphosphate + 4-O-dimethylallyl-D-tyrosine | 57.6% conversion | ? | |
dimethylallyl diphosphate + D-tyrosine | - |
Aspergillus niger | diphosphate + 4-O-dimethylallyl-D-tyrosine | 59.5% conversion | ? | |
dimethylallyl diphosphate + L-tyrosine | - |
Aspergillus niger | diphosphate + 4-O-dimethylallyl-L-tyrosine | 89.1% conversion | ? | |
dimethylallyl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | diphosphate + 4-O-dimethylallyl-L-tyrosine | 99.3% conversion | ? | |
methylallyl diphosphate + 4-amino-L-phenylalanine | - |
Leptosphaeria maculans | diphosphate + 4-(methylallylamino)-L-phenylalanine | 34.8% conversion | ? | |
methylallyl diphosphate + 4-amino-L-phenylalanine | - |
Aspergillus niger | diphosphate + 4-(methylallylamino)-L-phenylalanine | 57.7% conversion | ? | |
methylallyl diphosphate + L-tyrosine | - |
Leptosphaeria maculans | diphosphate + 4-O-methylallyl-L-tyrosine | 28% conversion | ? | |
methylallyl diphosphate + L-tyrosine | - |
Aspergillus niger | diphosphate + 4-O-methylallyl-L-tyrosine | 21.2% conversion | ? | |
additional information | in presence of unnatural dimethylallyl diphosphate analogs such as methylallyl, 2-pentenyl and benzyl diphosphate for alkylation, or benzylation of the indole ring enzyme catalyzes the regiospecific O- or N-alkylation or benzylation at position C4 of the aromatic ring | Aspergillus niger | ? | - |
? | |
additional information | in presence of unnatural dimethylallyl diphosphate analogs such as methylallyl, 2-pentenyl and benzyl diphosphate for alkylation, or benzylation of the indole ring enzyme catalyzes the regiospecific O- or N-alkylation or benzylation at position C4 of the aromatic ring | Leptosphaeria maculans | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SirD | - |
Leptosphaeria maculans |
TyrPT | - |
Aspergillus niger |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
methylallyl diphosphate | cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37°C | Aspergillus niger | |
0.002 | - |
2-pentenyl diphosphate | cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37°C | Leptosphaeria maculans | |
0.003 | - |
methylallyl diphosphate | cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37°C | Leptosphaeria maculans | |
0.003 | - |
2-pentenyl diphosphate | cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37°C | Aspergillus niger | |
0.016 | - |
benzyl diphosphate | cosubstrate 4-aminophenylalanine, pH 7.5, 37°C | Aspergillus niger | |
0.028 | - |
benzyl diphosphate | cosubstrate 4-aminophenylalanine, pH 7.5, 37°C | Leptosphaeria maculans | |
0.69 | - |
dimethylallyl diphosphate | pH 7.5, 37°C | Aspergillus niger | |
1 | - |
dimethylallyl diphosphate | pH 7.5, 37°C | Leptosphaeria maculans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
2-pentenyl diphosphate | cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37°C | Aspergillus niger | |
0.021 | - |
methylallyl diphosphate | cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37°C | Leptosphaeria maculans | |
0.031 | - |
benzyl diphosphate | cosubstrate 4-aminophenylalanine, pH 7.5, 37°C | Aspergillus niger | |
0.037 | - |
benzyl diphosphate | cosubstrate 4-aminophenylalanine, pH 7.5, 37°C | Leptosphaeria maculans | |
0.069 | - |
methylallyl diphosphate | cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37°C | Aspergillus niger | |
0.098 | - |
2-pentenyl diphosphate | cosubstrate 4-hydroxyphenylalanine, pH 7.5, 37°C | Leptosphaeria maculans | |
5.88 | - |
dimethylallyl diphosphate | pH 7.5, 37°C | Leptosphaeria maculans |