Cloned (Comment) | Organism |
---|---|
gene An13g01840, DNA and amino acid sequence determination and analysis, overexpression of His6-tagged enzyme in Escherichia coli strain SoluBL21, subcloning in Escherichia coli strain XL1 BlueMRF' | Aspergillus niger |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | strong inhibition | Aspergillus niger | |
Zn2+ | slight inhibition | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
L-tryptophan | at pH 7.5 and 37°C | Aspergillus niger | |
0.19 | - |
3,4-dihydroxy-L-phenylalanine | at pH 7.5 and 37°C | Aspergillus niger | |
0.22 | - |
D-tyrosine | at pH 7.5 and 37°C | Aspergillus niger | |
0.24 | - |
L-tyrosine | pH 7.5, 37°C | Aspergillus niger | |
0.24 | - |
L-tyrosine | at pH 7.5 and 37°C | Aspergillus niger | |
0.25 | - |
4-methyl-DL-tryptophan | at pH 7.5 and 37°C | Aspergillus niger | |
0.29 | - |
3-iodo-L-tyrosine | at pH 7.5 and 37°C | Aspergillus niger | |
0.42 | - |
alpha-methyl-L-tyrosine | at pH 7.5 and 37°C | Aspergillus niger | |
0.69 | - |
4-methyl-DL-tryptophan | at pH 7.5 and 37°C | Aspergillus niger | |
0.71 | - |
dimethylallyl diphosphate | pH 7.5, 37°C | Aspergillus niger | |
0.71 | - |
dimethylallyl diphosphate | with 3-iodo-L-tyrosine as cosubstrate, at pH 7.5 and 37°C | Aspergillus niger |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Aspergillus niger | |
Mg2+ | activates | Aspergillus niger | |
additional information | addition of K+, Na+, Mg2+, Mn2+, Co2+, or EDTA to the reaction mixture does not change the enzyme activity significantly | Aspergillus niger | |
additional information | no significant effects on enzyme activity by K+, Na+, Mg2+, Mn2+, Co2+, or EDTA | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52700 | - |
- |
Aspergillus niger |
52700 | - |
x * 52700, calculated from amino acid sequence | Aspergillus niger |
53500 | - |
- |
Aspergillus niger |
228000 | - |
recombinant His6-tagged enzyme, gel filtration | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + L-tyrosine | Aspergillus niger | - |
diphosphate + 4-O-dimethylallyl-L-tyrosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger | A2R1N3 | gene An13g01840 or ANI_1_660114 | - |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21 to near homogeneity by nickel affinity chromatography and desalting gel filtration | Aspergillus niger |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus niger | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 3,4-dihydroxy-L-phenylalanine | 98% activity compared to 3-iodo-L-tyrosine | Aspergillus niger | ? | - |
? | |
dimethylallyl diphosphate + 3,4-dihydroxy-L-phenylalanine | - |
Aspergillus niger | diphosphate + ? | - |
? | |
dimethylallyl diphosphate + 3-fluoro-DL-tyrosine | 53% activity compared to 3-iodo-L-tyrosine | Aspergillus niger | ? | - |
? | |
dimethylallyl diphosphate + 3-iodo-L-tyrosine | 100% activity | Aspergillus niger | ? | - |
? | |
dimethylallyl diphosphate + 3-iodo-L-tyrosine | - |
Aspergillus niger | diphosphate + ? | - |
? | |
dimethylallyl diphosphate + 4-amino-L-phenylalanine | 23% activity compared to 3-iodo-L-tyrosine | Aspergillus niger | ? | - |
? | |
dimethylallyl diphosphate + 4-amino-L-phenylalanine | prenylation at 4-O- or N-atom | Aspergillus niger | diphosphate + 4-dimethylallylamino-L-phenylalanine | - |
? | |
dimethylallyl diphosphate + 4-methyl-DL-tryptophan | 13% activity compared to 3-iodo-L-tyrosine | Aspergillus niger | ? | - |
? | |
dimethylallyl diphosphate + 4-methyl-DL-tryptophan | 41% activity compared to 3-iodo-L-tyrosine | Aspergillus niger | diphosphate + 7-dimethylallyl-4-methyl-L-tryptophan | - |
? | |
dimethylallyl diphosphate + 5-methyl-DL-tryptophan | 10% activity compared to 3-iodo-L-tyrosine | Aspergillus niger | diphosphate + 7-dimethylally-5-methyl-L-tryptophan | - |
? | |
dimethylallyl diphosphate + alpha-methyl-L-tyrosine | 98% activity compared to 3-iodo-L-tyrosine | Aspergillus niger | ? | - |
? | |
dimethylallyl diphosphate + D-tyrosine | 33% activity compared to 3-iodo-L-tyrosine | Aspergillus niger | ? | - |
? | |
dimethylallyl diphosphate + D-tyrosine | - |
Aspergillus niger | diphosphate + 4-O-dimethylallyl-D-tyrosine | - |
? | |
dimethylallyl diphosphate + L-tryptophan | 33% activity compared to 3-iodo-L-tyrosine | Aspergillus niger | diphosphate + 7-dimethylallyl-L-tryptophan | - |
? | |
dimethylallyl diphosphate + L-tyrosine | - |
Aspergillus niger | diphosphate + 4-O-dimethylallyl-L-tyrosine | - |
? | |
dimethylallyl diphosphate + L-tyrosine | 98% activity compared to 3-iodo-L-tyrosine | Aspergillus niger | diphosphate + 4-O-dimethylallyl-L-tyrosine | - |
? | |
additional information | the enzyme shows a wider substrate spectrum compared to tyrosine prenyltransferase SirD. It catalyzes O-prenylation of L-tyrosine, EC 2.5.1.122, and also accepts 4-amino-L-phenylalanine for an N-prenylation and L-tryptophan for a C7-prenylation, EC 2.5.1.80. It is also active with 4-amino-L-phenylalanine as well as 4- and 5-methyl-DL-tryptophan. HRMS analysis of the enzyme products, overview. 3,5-dibromo-L-tyrosine is a poor substrate | Aspergillus niger | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52700, calculated from amino acid sequence | Aspergillus niger |
? | x * 53500, recombinant His6-tagged enzyme, SDS-PAGE, x * 52700, about, sequence calculation | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
ANI_1_660114 | - |
Aspergillus niger |
CAK41583 | - |
Aspergillus niger |
tyrosine O-prenyltransferase | - |
Aspergillus niger |
TyrPT | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Aspergillus niger |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0036 | - |
4-methyl-DL-tryptophan | at pH 7.5 and 37°C | Aspergillus niger | |
0.0053 | - |
L-tryptophan | at pH 7.5 and 37°C | Aspergillus niger | |
0.16 | - |
D-tyrosine | at pH 7.5 and 37°C | Aspergillus niger | |
0.16 | - |
3-iodo-L-tyrosine | at pH 7.5 and 37°C | Aspergillus niger | |
0.2 | - |
4-methyl-DL-tryptophan | at pH 7.5 and 37°C | Aspergillus niger | |
0.26 | - |
alpha-methyl-L-tyrosine | at pH 7.5 and 37°C | Aspergillus niger | |
0.27 | - |
3,4-dihydroxy-L-phenylalanine | at pH 7.5 and 37°C | Aspergillus niger | |
0.58 | - |
L-tyrosine | pH 7.5, 37°C | Aspergillus niger | |
0.58 | - |
L-tyrosine | at pH 7.5 and 37°C | Aspergillus niger | |
0.69 | - |
dimethylallyl diphosphate | with 3-iodo-L-tyrosine as cosubstrate, at pH 7.5 and 37°C | Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Aspergillus niger |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is a member of the DMATS superfamily | Aspergillus niger |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
L-tryptophan | at pH 7.5 and 37°C | Aspergillus niger | |
0.043 | - |
4-methyl-DL-tryptophan | at pH 7.5 and 37°C | Aspergillus niger | |
0.551 | - |
3-iodo-L-tyrosine | at pH 7.5 and 37°C | Aspergillus niger | |
0.619 | - |
alpha-methyl-L-tyrosine | at pH 7.5 and 37°C | Aspergillus niger | |
0.727 | - |
D-tyrosine | at pH 7.5 and 37°C | Aspergillus niger | |
0.8 | - |
4-methyl-DL-tryptophan | at pH 7.5 and 37°C | Aspergillus niger | |
0.972 | - |
dimethylallyl diphosphate | with 3-iodo-L-tyrosine as cosubstrate, at pH 7.5 and 37°C | Aspergillus niger | |
1.421 | - |
3,4-dihydroxy-L-phenylalanine | at pH 7.5 and 37°C | Aspergillus niger |