Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli B834(DE3) and BL21(DE3) cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
native enzyme bound to CysO sulfur-carrier protein, hanging drop vapor diffusion method, using 7-10% PEG (w/v) 4000, 0.1 M sodium citrate, pH 5.8, and 0.2 M ammonium acetate at 22°C | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH | Mycobacterium tuberculosis | - |
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH | Mycobacterium tuberculosis H37Rv | - |
[CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + acetate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH | - |
Mycobacterium tuberculosis | [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + acetate | - |
? | |
O-acetyl-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH | - |
Mycobacterium tuberculosis H37Rv | [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
CysM | - |
Mycobacterium tuberculosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Mycobacterium tuberculosis |