Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.5.1.113 extracted from

  • Agren, D.; Schnell, R.; Oehlmann, W.; Singh, M.; Schneider, G.
    Cysteine synthase (CYSM) of Mycobacterium tuberculosis is an O-phosphoserine sulfhydrylase: Evidence for an alternative cysteine biosynthesis pathway in mycobacteria (2008), J. Biol. Chem., 283, 31567-31574.
    View publication on PubMed
Search for more literature for 2.5.1.113

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M Tris-HCl, pH 7.25-7.5, 0.1 M K2HPO4, 4.3 M NaCl Mycobacterium tuberculosis

Protein Variants

Protein Variants Comment Organism
R220A the mutant shows reduced activity with O-phospho-L-serine and increased activity with O-acetyl-L-serine compared to the wild type enzyme Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH Mycobacterium tuberculosis
-
 [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
 ?
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH Mycobacterium tuberculosis H37Rv
-
 [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis P9WP53
-
-
Mycobacterium tuberculosis H37Rv P9WP53
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, Superdex-200 gel filtration, and Sephadex G-25 gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with O-acetyl-L-serine Mycobacterium tuberculosis ?
-
 ?
additional information no activity with O-acetyl-L-serine Mycobacterium tuberculosis H37Rv ?
-
 ?
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
-
 Mycobacterium tuberculosis [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
 ?
O-phospho-L-serine + [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)SH
-
 Mycobacterium tuberculosis H37Rv [CysO sulfur-carrier protein]-Gly-NH-CH2-C(O)-S-L-cysteine + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
CysM
-
 Mycobacterium tuberculosis
O-phosphoserine sulfhydrylase
-
 Mycobacterium tuberculosis
O-phosphoserine-specific cysteine synthase
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 O-phospho-L-serine at pH 7.5 and 37°C Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate dependent on Mycobacterium tuberculosis