Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptomyces niveus |
Protein Variants | Comment | Organism |
---|---|---|
E281A | the mutant shows about 10% activity compared to the wild type enzyme | Streptomyces niveus |
E281D | the mutant shows about 78% activity compared to the wild type enzyme | Streptomyces niveus |
E281K | the mutant shows about 60% activity compared to the wild type enzyme | Streptomyces niveus |
E281M | the mutant shows about 98% activity compared to the wild type enzyme | Streptomyces niveus |
E281Q | the mutant shows 205% activity compared to the wild type enzyme | Streptomyces niveus |
E281R | the mutant shows about 5% activity compared to the wild type enzyme | Streptomyces niveus |
E281S | the mutant shows about 30% activity compared to the wild type enzyme | Streptomyces niveus |
E281T | the mutant shows about 40% activity compared to the wild type enzyme | Streptomyces niveus |
E281V | the mutant shows about 78% activity compared to the wild type enzyme | Streptomyces niveus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
menadione hydroquinol | the relative activity is drastically decreased when the reaction system is supplemented with more than 1.5 mM of menadione hydroquinol. 3 mM menadione hydroquinol almost absolutely inhibits the prenylation by precipitating the enzyme in the reaction system | Streptomyces niveus | |
additional information | nearly full activity is obtained in the presence of 10 mM EDTA | Streptomyces niveus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1044 | - |
menadione hydroquinol | mutant enzyme E281R, at pH 8.0 and 32°C | Streptomyces niveus | |
0.1537 | - |
menadione hydroquinol | mutant enzyme E281Q, at pH 8.0 and 32°C | Streptomyces niveus | |
0.1737 | - |
menadione hydroquinol | wild type enzyme, at pH 8.0 and 32°C | Streptomyces niveus | |
0.2106 | - |
menadione hydroquinol | mutant enzyme E281M, at pH 8.0 and 32°C | Streptomyces niveus | |
0.2181 | - |
menadione hydroquinol | mutant enzyme E281K, at pH 8.0 and 32°C | Streptomyces niveus | |
0.2933 | - |
menadione hydroquinol | mutant enzyme E281V, at pH 8.0 and 32°C | Streptomyces niveus | |
0.3623 | - |
menadione hydroquinol | mutant enzyme E281A, at pH 8.0 and 32°C | Streptomyces niveus | |
0.4097 | - |
menadione hydroquinol | mutant enzyme E281S, at pH 8.0 and 32°C | Streptomyces niveus | |
0.4799 | - |
menadione hydroquinol | mutant enzyme E281T, at pH 8.0 and 32°C | Streptomyces niveus | |
0.5202 | - |
menadione hydroquinol | mutant enzyme E281D, at pH 8.0 and 32°C | Streptomyces niveus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 2.5 mM used in assay conditions | Streptomyces niveus | |
additional information | the enzyme is independent of metal ions | Streptomyces niveus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate | Streptomyces niveus | - |
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate | - |
? | |
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate | Streptomyces niveus CPCC 240348 | - |
diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces niveus | Q9L9F1 | - |
- |
Streptomyces niveus CPCC 240348 | Q9L9F1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate | - |
Streptomyces niveus | diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate | - |
? | |
dimethylallyl diphosphate + 4-hydroxyphenylpyruvate | - |
Streptomyces niveus CPCC 240348 | diphosphate + 3-dimethylallyl-4-hydroxyphenylpyruvate | - |
? | |
dimethylallyl diphosphate + menadione hydroquinol | - |
Streptomyces niveus | diphosphate + vitamin MK1 | - |
? | |
dimethylallyl diphosphate + menadione hydroquinol | - |
Streptomyces niveus CPCC 240348 | diphosphate + vitamin MK1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NovQ | - |
Streptomyces niveus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
32 | - |
- |
Streptomyces niveus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
menadione hydroquinol | mutant enzyme E281R, at pH 8.0 and 32°C | Streptomyces niveus | |
0.005 | - |
menadione hydroquinol | mutant enzyme E281A, at pH 8.0 and 32°C | Streptomyces niveus | |
0.014 | - |
menadione hydroquinol | mutant enzyme E281S, at pH 8.0 and 32°C | Streptomyces niveus | |
0.022 | - |
menadione hydroquinol | mutant enzyme E281K, at pH 8.0 and 32°C | Streptomyces niveus | |
0.026 | - |
menadione hydroquinol | mutant enzyme E281T, at pH 8.0 and 32°C | Streptomyces niveus | |
0.038 | - |
menadione hydroquinol | mutant enzyme E281V, at pH 8.0 and 32°C | Streptomyces niveus | |
0.038 | - |
menadione hydroquinol | wild type enzyme, at pH 8.0 and 32°C | Streptomyces niveus | |
0.039 | - |
menadione hydroquinol | mutant enzyme E281M, at pH 8.0 and 32°C | Streptomyces niveus | |
0.045 | - |
menadione hydroquinol | mutant enzyme E281D, at pH 8.0 and 32°C | Streptomyces niveus | |
0.083 | - |
menadione hydroquinol | mutant enzyme E281Q, at pH 8.0 and 32°C | Streptomyces niveus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Streptomyces niveus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
menadione hydroquinol | mutant enzyme E281A, at pH 8.0 and 32°C | Streptomyces niveus | |
0.029 | - |
menadione hydroquinol | mutant enzyme E281R, at pH 8.0 and 32°C | Streptomyces niveus | |
0.054 | - |
menadione hydroquinol | mutant enzyme E281T, at pH 8.0 and 32°C | Streptomyces niveus | |
0.064 | - |
menadione hydroquinol | mutant enzyme E281S, at pH 8.0 and 32°C | Streptomyces niveus | |
0.087 | - |
menadione hydroquinol | mutant enzyme E281D, at pH 8.0 and 32°C | Streptomyces niveus | |
0.1 | - |
menadione hydroquinol | mutant enzyme E281K, at pH 8.0 and 32°C | Streptomyces niveus | |
0.13 | - |
menadione hydroquinol | mutant enzyme E281V, at pH 8.0 and 32°C | Streptomyces niveus | |
0.185 | - |
menadione hydroquinol | mutant enzyme E281M, at pH 8.0 and 32°C | Streptomyces niveus | |
0.219 | - |
menadione hydroquinol | wild type enzyme, at pH 8.0 and 32°C | Streptomyces niveus | |
0.54 | - |
menadione hydroquinol | mutant enzyme E281Q, at pH 8.0 and 32°C | Streptomyces niveus |