BRENDA - Enzyme Database show
show all sequences of 2.5.1.109

C7-prenylation of tryptophanyl and O-prenylation of tyrosyl residues in dipeptides by an Aspergillus terreus prenyltransferase

Wunsch, C.; Zou, H.X.; Linne, U.; Li, S.M.; Appl. Microbiol. Biotechnol. 99, 1719-1730 (2015)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
regularly C7-prenylated cyclic dipeptides are successfully obtained by using the member of the DMATS family, CdpC7PT from Aspergillus terreus
Aspergillus terreus
Cloned(Commentary)
Commentary
Organism
gene ATEG_04218 or cdpC7PT, cloning from genomic DNA, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain M15
Aspergillus terreus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics, for the tryptophan-containing cyclic dipeptides, KM values between 0.239 and 0.747 mM and turnover numbers in the range of 0.009-0.049/s are determined
Aspergillus terreus
0.0785
-
dimethylallyl diphosphate
pH 7.5, 37°C
Aspergillus terreus
0.204
-
(S)-benzodiazepinedione
pH 7.5, 37°C
Aspergillus terreus
1.411
-
cyclo-L-Tyr-L-Tyr
pH 7.5, 37°C
Aspergillus terreus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
activates 58% at 5 mM
Aspergillus terreus
Mg2+
activates 20% at 5 mM
Aspergillus terreus
Mn2+
activates 31% at 5 mM
Aspergillus terreus
additional information
no significant effect by 5 mM EDTA. The enzyme reaction of CdpC7PT is independent of metal ions, but can be enhanced by Ca2+, Mn2+, and Mg2+
Aspergillus terreus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62000
-
recombinant His6-tagged enzyme, gel filtration
Aspergillus terreus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dimethylallyl diphosphate + cyclo-L-Trp-L-Trp
Aspergillus terreus
attachment of the prenyl moiety to C-7 of the indole ring
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Trp
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-Xaa
Aspergillus oryzae
-
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-Xaa
-
-
?
additional information
Aspergillus terreus
for some substrates, reversely C3- or N1-prenylated derivatives are identified as minor products
?
-
-
-
additional information
Aspergillus terreus DSM 1958
for some substrates, reversely C3- or N1-prenylated derivatives are identified as minor products
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus oryzae
-
gene cdpC7PT
-
Aspergillus terreus
Q0CQ16
gene ATEG_04218 or cdpC7PT
-
Aspergillus terreus DSM 1958
Q0CQ16
gene ATEG_04218 or cdpC7PT
-
Purification (Commentary)
Commentary
Organism
recombinant His6-tagged enzyme from Escherichia coli strain M15 by nickel affinity chromatography
Aspergillus terreus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus oryzae
-
mycelium
-
Aspergillus terreus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dimethylallyl diphosphate + (R)-benzodiazepinedione
low activity, (R)-benzodiazepinedione consists of a tryptophanyl and an anthranilyl moiety
737541
Aspergillus terreus
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + (S)-benzodiazepinedione
best substrate, (S)-benzodiazepinedione consists of a tryptophanyl and an anthranilyl moiety
737541
Aspergillus terreus
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + benzodiazepinedione
best substrate, active only with benzodiazepinedione consisting of a D-tryptophanyl and an anthranilyl moiety, poor activity with the L-Tryp isomer
737541
Aspergillus terreus
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-D-Pro
-
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-D-Trp-D-Pro
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-D-Pro
low activity
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-D-Trp-D-Pro
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-L-Ala
-
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-D-Trp-L-Ala
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-L-Pro
low activity
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-D-Trp-L-Pro
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-L-Trp
low activity
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-D-Trp-L-Trp
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-D-Ala
low activity
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-D-Ala
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-D-Pro
-
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-D-Pro
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-Gly
-
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-Gly
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Ala
-
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Ala
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Leu
-
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Leu
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Pro
-
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Pro
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Trp
-
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Trp
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Trp
higher activity
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Trp
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Trp
attachment of the prenyl moiety to C-7 of the indole ring
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Trp
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Trp
-
737541
Aspergillus terreus DSM 1958
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Trp
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Tyr
-
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Tyr
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Tyr
higher activity
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Tyr
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-Xaa
-
737541
Aspergillus oryzae
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-Xaa
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Tyr-L-Tyr
-
737541
Aspergillus terreus
diphosphate + 4-O-(3-methylbut-2-enyl)-cyclo-L-Tyr-L-Tyr
-
-
-
?
dimethylallyl diphosphate + cyclo-LTrp-L-Phe
-
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Phe
-
-
-
?
dimethylallyl diphosphate + L-tryptophan
reaction of 7-DMATS, EC 2.5.1.80, low activity
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
-
-
-
?
dimethylallyl diphosphate + L-tryptophan
reaction of 7-DMATS, EC 2.5.1.80, low activity
737541
Aspergillus terreus DSM 1958
diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
-
-
-
?
additional information
for some substrates, reversely C3- or N1-prenylated derivatives are identified as minor products
737541
Aspergillus terreus
?
-
-
-
-
additional information
the enzyme uses cyclic dipeptides containing L-Trp and attaches the prenyl moiety to C-7 of the indole ring, no activity with cyclo-L-Trp-L-Pro, cyclo-L-Trp-D-Pro, cyclo-D-Trp-L-Pro, cyclo-D-Trp-D-Pro, and cyclo-L-Trp-Gly
737541
Aspergillus terreus
?
-
-
-
-
additional information
the enzyme uses cyclic dipeptides containing L-Trp and attaches the prenyl moiety to C-7 of the indole ring. It also accepts cyclo-L-Tyr-L-Tyr as substrate and catalyzes an O-prenylation at the tyrosyl residue of the dipeptide cyclo-L-Trp-L-Tyr. Substrate specificity, overview. Cyclo-L-Tyr-L-Ser, cyclo-L-Tyr-L-Pro, cyclo-L-Tyr-Gly, and L-tyrosine are poor substrates. CdpC7PT shows a high affinity to its prenyl donor dimethylallyl diphosphate
737541
Aspergillus terreus
?
-
-
-
-
additional information
the enzyme uses cyclic dipeptides containing L-Trp and attaches the prenyl moiety to C-7 of the indole ring. The enzyme showsma relatively narrow substrate specificity
737541
Aspergillus oryzae
?
-
-
-
-
additional information
for some substrates, reversely C3- or N1-prenylated derivatives are identified as minor products
737541
Aspergillus terreus DSM 1958
?
-
-
-
-
additional information
the enzyme uses cyclic dipeptides containing L-Trp and attaches the prenyl moiety to C-7 of the indole ring, no activity with cyclo-L-Trp-L-Pro, cyclo-L-Trp-D-Pro, cyclo-D-Trp-L-Pro, cyclo-D-Trp-D-Pro, and cyclo-L-Trp-Gly
737541
Aspergillus terreus DSM 1958
?
-
-
-
-
additional information
the enzyme uses cyclic dipeptides containing L-Trp and attaches the prenyl moiety to C-7 of the indole ring. It also accepts cyclo-L-Tyr-L-Tyr as substrate and catalyzes an O-prenylation at the tyrosyl residue of the dipeptide cyclo-L-Trp-L-Tyr. Substrate specificity, overview. Cyclo-L-Tyr-L-Ser, cyclo-L-Tyr-L-Pro, cyclo-L-Tyr-Gly, and L-tyrosine are poor substrates. CdpC7PT shows a high affinity to its prenyl donor dimethylallyl diphosphate
737541
Aspergillus terreus DSM 1958
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Aspergillus terreus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.012
-
cyclo-L-Tyr-L-Tyr
pH 7.5, 37°C
Aspergillus terreus
0.067
-
dimethylallyl diphosphate
pH 7.5, 37°C
Aspergillus terreus
0.125
-
(S)-benzodiazepinedione
pH 7.5, 37°C
Aspergillus terreus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Aspergillus terreus
Application (protein specific)
Application
Commentary
Organism
synthesis
regularly C7-prenylated cyclic dipeptides are successfully obtained by using the member of the DMATS family, CdpC7PT from Aspergillus terreus
Aspergillus terreus
Cloned(Commentary) (protein specific)
Commentary
Organism
gene ATEG_04218 or cdpC7PT, cloning from genomic DNA, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain M15
Aspergillus terreus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics, for the tryptophan-containing cyclic dipeptides, KM values between 0.239 and 0.747 mM and turnover numbers in the range of 0.009-0.049/s are determined
Aspergillus terreus
0.0785
-
dimethylallyl diphosphate
pH 7.5, 37°C
Aspergillus terreus
0.204
-
(S)-benzodiazepinedione
pH 7.5, 37°C
Aspergillus terreus
1.411
-
cyclo-L-Tyr-L-Tyr
pH 7.5, 37°C
Aspergillus terreus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
activates 58% at 5 mM
Aspergillus terreus
Mg2+
activates 20% at 5 mM
Aspergillus terreus
Mn2+
activates 31% at 5 mM
Aspergillus terreus
additional information
no significant effect by 5 mM EDTA. The enzyme reaction of CdpC7PT is independent of metal ions, but can be enhanced by Ca2+, Mn2+, and Mg2+
Aspergillus terreus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
62000
-
recombinant His6-tagged enzyme, gel filtration
Aspergillus terreus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dimethylallyl diphosphate + cyclo-L-Trp-L-Trp
Aspergillus terreus
attachment of the prenyl moiety to C-7 of the indole ring
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Trp
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-Xaa
Aspergillus oryzae
-
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-Xaa
-
-
?
additional information
Aspergillus terreus
for some substrates, reversely C3- or N1-prenylated derivatives are identified as minor products
?
-
-
-
additional information
Aspergillus terreus DSM 1958
for some substrates, reversely C3- or N1-prenylated derivatives are identified as minor products
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His6-tagged enzyme from Escherichia coli strain M15 by nickel affinity chromatography
Aspergillus terreus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
mycelium
-
Aspergillus oryzae
-
mycelium
-
Aspergillus terreus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dimethylallyl diphosphate + (R)-benzodiazepinedione
low activity, (R)-benzodiazepinedione consists of a tryptophanyl and an anthranilyl moiety
737541
Aspergillus terreus
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + (S)-benzodiazepinedione
best substrate, (S)-benzodiazepinedione consists of a tryptophanyl and an anthranilyl moiety
737541
Aspergillus terreus
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + benzodiazepinedione
best substrate, active only with benzodiazepinedione consisting of a D-tryptophanyl and an anthranilyl moiety, poor activity with the L-Tryp isomer
737541
Aspergillus terreus
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-D-Pro
-
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-D-Trp-D-Pro
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-D-Pro
low activity
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-D-Trp-D-Pro
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-L-Ala
-
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-D-Trp-L-Ala
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-L-Pro
low activity
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-D-Trp-L-Pro
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-L-Trp
low activity
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-D-Trp-L-Trp
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-D-Ala
low activity
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-D-Ala
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-D-Pro
-
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-D-Pro
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-Gly
-
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-Gly
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Ala
-
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Ala
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Leu
-
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Leu
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Pro
-
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Pro
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Trp
-
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Trp
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Trp
higher activity
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Trp
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Trp
attachment of the prenyl moiety to C-7 of the indole ring
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Trp
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Trp
-
737541
Aspergillus terreus DSM 1958
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Trp
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Tyr
-
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Tyr
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Tyr
higher activity
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Tyr
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-Xaa
-
737541
Aspergillus oryzae
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-Xaa
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Tyr-L-Tyr
-
737541
Aspergillus terreus
diphosphate + 4-O-(3-methylbut-2-enyl)-cyclo-L-Tyr-L-Tyr
-
-
-
?
dimethylallyl diphosphate + cyclo-LTrp-L-Phe
-
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-cyclo-L-Trp-L-Phe
-
-
-
?
dimethylallyl diphosphate + L-tryptophan
reaction of 7-DMATS, EC 2.5.1.80, low activity
737541
Aspergillus terreus
diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
-
-
-
?
dimethylallyl diphosphate + L-tryptophan
reaction of 7-DMATS, EC 2.5.1.80, low activity
737541
Aspergillus terreus DSM 1958
diphosphate + 7-(3-methylbut-2-enyl)-L-tryptophan
-
-
-
?
additional information
for some substrates, reversely C3- or N1-prenylated derivatives are identified as minor products
737541
Aspergillus terreus
?
-
-
-
-
additional information
the enzyme uses cyclic dipeptides containing L-Trp and attaches the prenyl moiety to C-7 of the indole ring, no activity with cyclo-L-Trp-L-Pro, cyclo-L-Trp-D-Pro, cyclo-D-Trp-L-Pro, cyclo-D-Trp-D-Pro, and cyclo-L-Trp-Gly
737541
Aspergillus terreus
?
-
-
-
-
additional information
the enzyme uses cyclic dipeptides containing L-Trp and attaches the prenyl moiety to C-7 of the indole ring. It also accepts cyclo-L-Tyr-L-Tyr as substrate and catalyzes an O-prenylation at the tyrosyl residue of the dipeptide cyclo-L-Trp-L-Tyr. Substrate specificity, overview. Cyclo-L-Tyr-L-Ser, cyclo-L-Tyr-L-Pro, cyclo-L-Tyr-Gly, and L-tyrosine are poor substrates. CdpC7PT shows a high affinity to its prenyl donor dimethylallyl diphosphate
737541
Aspergillus terreus
?
-
-
-
-
additional information
the enzyme uses cyclic dipeptides containing L-Trp and attaches the prenyl moiety to C-7 of the indole ring. The enzyme showsma relatively narrow substrate specificity
737541
Aspergillus oryzae
?
-
-
-
-
additional information
for some substrates, reversely C3- or N1-prenylated derivatives are identified as minor products
737541
Aspergillus terreus DSM 1958
?
-
-
-
-
additional information
the enzyme uses cyclic dipeptides containing L-Trp and attaches the prenyl moiety to C-7 of the indole ring, no activity with cyclo-L-Trp-L-Pro, cyclo-L-Trp-D-Pro, cyclo-D-Trp-L-Pro, cyclo-D-Trp-D-Pro, and cyclo-L-Trp-Gly
737541
Aspergillus terreus DSM 1958
?
-
-
-
-
additional information
the enzyme uses cyclic dipeptides containing L-Trp and attaches the prenyl moiety to C-7 of the indole ring. It also accepts cyclo-L-Tyr-L-Tyr as substrate and catalyzes an O-prenylation at the tyrosyl residue of the dipeptide cyclo-L-Trp-L-Tyr. Substrate specificity, overview. Cyclo-L-Tyr-L-Ser, cyclo-L-Tyr-L-Pro, cyclo-L-Tyr-Gly, and L-tyrosine are poor substrates. CdpC7PT shows a high affinity to its prenyl donor dimethylallyl diphosphate
737541
Aspergillus terreus DSM 1958
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Aspergillus terreus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.012
-
cyclo-L-Tyr-L-Tyr
pH 7.5, 37°C
Aspergillus terreus
0.067
-
dimethylallyl diphosphate
pH 7.5, 37°C
Aspergillus terreus
0.125
-
(S)-benzodiazepinedione
pH 7.5, 37°C
Aspergillus terreus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Aspergillus terreus
General Information
General Information
Commentary
Organism
evolution
the enzyme belongs to the DMATS superfamily
Aspergillus oryzae
evolution
the enzyme belongs to the DMATS superfamily
Aspergillus terreus
General Information (protein specific)
General Information
Commentary
Organism
evolution
the enzyme belongs to the DMATS superfamily
Aspergillus oryzae
evolution
the enzyme belongs to the DMATS superfamily
Aspergillus terreus
Other publictions for EC 2.5.1.109
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737541
Wunsch
C7-prenylation of tryptophanyl ...
Aspergillus oryzae, Aspergillus terreus, Aspergillus terreus DSM 1958
Appl. Microbiol. Biotechnol.
99
1719-1730
2015
-
1
1
-
-
-
-
4
-
4
1
4
-
6
-
-
1
-
-
2
-
-
32
-
1
-
-
3
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
4
-
4
1
4
-
-
-
1
-
2
-
-
32
-
1
-
-
3
1
-
-
-
-
2
2
-
-
-
721391
Yin
Identification of a brevianami ...
Aspergillus versicolor, Aspergillus versicolor NRRL 573, Aspergillus versicolor NRRL573
Appl. Microbiol. Biotechnol.
97
1649-1660
2013
-
-
1
-
-
-
-
14
-
-
1
3
-
7
-
-
1
-
-
-
-
-
36
1
-
-
-
14
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
14
-
-
1
3
-
-
-
1
-
-
-
-
36
1
-
-
-
14
-
-
-
-
-
-
-
-
15
15
722446
Ding
Genome-based characterization ...
Aspergillus sp., Aspergillus sp. MF297-2
J. Am. Chem. Soc.
132
12733-12740
2010
-
-
1
-
6
-
4
2
-
3
2
2
-
2
-
-
-
-
-
-
-
-
4
1
1
1
-
2
1
1
-
-
-
-
-
-
-
1
-
-
6
-
-
4
-
2
-
3
2
2
-
-
-
-
-
-
-
-
4
1
1
1
-
2
1
1
-
-
-
-
-
-
2
2