BRENDA - Enzyme Database show
show all sequences of 2.5.1.109

Identification of a brevianamide F reverse prenyltransferase BrePT from Aspergillus versicolor with a broad substrate specificity towards tryptophan-containing cyclic dipeptides

Yin, S.; Yu, X.; Wang, Q.; Liu, X.Q.; Li, S.M.; Appl. Microbiol. Biotechnol. 97, 1649-1660 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpression in Escherichia coli
Aspergillus versicolor
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.032
-
brevianamide F
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.082
-
cyclo-L-Trp-D-Pro
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.094
-
cyclo-L-Trp-L-Phe
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.098
-
dimethylallyl diphosphate
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.106
-
cyclo-L-Trp-L-Leu
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.139
-
cyclo-D-Trp-L-Ala
pH 7.5, 37°C
Aspergillus versicolor
0.214
-
cyclo-L-Trp-L-Tyr
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.709
-
cyclo-D-Trp-D-Pro
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.793
-
cyclo-L-Trp-D-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.942
-
cyclo-L-Trp-L-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
1.3
-
cyclo-L-Trp-Gly
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
1.318
-
cyclo-D-Trp-D-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
1.318
-
cyclo-D-Trp-L-Ala
37°C, pH 7.5
Aspergillus versicolor
2.906
-
cyclo-D-Trp-L-Pro
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
x * 45000, SDS-PAGE
Aspergillus versicolor
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dimethylallyl diphosphate + brevianamide F
Aspergillus versicolor
-
diphosphate + deoxybrevianamide E
-
-
?
dimethylallyl diphosphate + brevianamide F
Aspergillus versicolor NRRL 573
-
diphosphate + deoxybrevianamide E
-
-
?
dimethylallyl diphosphate + brevianamide F
Aspergillus versicolor NRRL573
-
diphosphate + deoxybrevianamide E
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus versicolor
I4AY86
-
-
Aspergillus versicolor NRRL 573
I4AY86
-
-
Aspergillus versicolor NRRL573
I4AY86
-
-
Purification (Commentary)
Commentary
Organism
-
Aspergillus versicolor
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dimethylallyl diphosphate + brevianamide F
-
721391
Aspergillus versicolor
diphosphate + deoxybrevianamide E
-
-
-
?
dimethylallyl diphosphate + brevianamide F
the enzyme accepts a broad range of tryptophan-containing cyclic dipeptides, no activity with farnesyl diphosphate and geranyl diphosphate
721391
Aspergillus versicolor
diphosphate + deoxybrevianamide E
-
-
-
?
dimethylallyl diphosphate + brevianamide F
i.e. cyclo-L-Trp-L-Pro
721391
Aspergillus versicolor
diphosphate + deoxybrevianamide E
-
-
-
?
dimethylallyl diphosphate + brevianamide F
-
721391
Aspergillus versicolor NRRL 573
diphosphate + deoxybrevianamide E
-
-
-
?
dimethylallyl diphosphate + brevianamide F
the enzyme accepts a broad range of tryptophan-containing cyclic dipeptides, no activity with farnesyl diphosphate and geranyl diphosphate
721391
Aspergillus versicolor NRRL 573
diphosphate + deoxybrevianamide E
-
-
-
?
dimethylallyl diphosphate + brevianamide F
i.e. cyclo-L-Trp-L-Pro
721391
Aspergillus versicolor NRRL 573
diphosphate + deoxybrevianamide E
-
-
-
?
dimethylallyl diphosphate + brevianamide F
-
721391
Aspergillus versicolor NRRL573
diphosphate + deoxybrevianamide E
-
-
-
?
dimethylallyl diphosphate + brevianamide F
the enzyme accepts a broad range of tryptophan-containing cyclic dipeptides, no activity with farnesyl diphosphate and geranyl diphosphate
721391
Aspergillus versicolor NRRL573
diphosphate + deoxybrevianamide E
-
-
-
?
dimethylallyl diphosphate + brevianamide F
i.e. cyclo-L-Trp-L-Pro
721391
Aspergillus versicolor NRRL573
diphosphate + deoxybrevianamide E
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-D-Ala
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-D-Ala
-
721391
Aspergillus versicolor NRRL 573
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-D-Ala
-
721391
Aspergillus versicolor NRRL573
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-D-Ala
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-D-Pro
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-D-Pro
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-L-Ala
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-L-Ala
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-L-Pro
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-L-Pro
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-D-Ala
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-D-Ala
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-D-Pro
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-D-Pro
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-Gly
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-Gly
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Ala
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Ala
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Leu
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Leu
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Phe
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Phe
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Tyr
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Tyr
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
additional information
enzyme shows high flexibility towards its aromatic substrates and accepts all of the 14 tested tryptophan-containing cyclic dipeptides. The enzyme catalyzes the highly regiospecific reverse prenylation at C2 of the indole nucleus
721391
Aspergillus versicolor
?
-
-
-
-
additional information
enzyme shows high flexibility towards its aromatic substrates and accepts all of the 14 tested tryptophan-containing cyclic dipeptides. The enzyme catalyzes the highly regiospecific reverse prenylation at C2 of the indole nucleus
721391
Aspergillus versicolor NRRL 573
?
-
-
-
-
additional information
enzyme shows high flexibility towards its aromatic substrates and accepts all of the 14 tested tryptophan-containing cyclic dipeptides. The enzyme catalyzes the highly regiospecific reverse prenylation at C2 of the indole nucleus
721391
Aspergillus versicolor NRRL573
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 45000, SDS-PAGE
Aspergillus versicolor
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.003
-
cyclo-D-Trp-L-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.004
-
cyclo-L-Trp-L-Phe
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.006
-
cyclo-L-Trp-L-Tyr
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.011
-
cyclo-D-Trp-D-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.013
-
cyclo-L-Trp-D-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.014
-
cyclo-L-Trp-L-Leu
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.027
-
cyclo-D-Trp-L-Pro
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.032
-
cyclo-L-Trp-Gly
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.035
-
cyclo-L-Trp-L-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.095
-
cyclo-D-Trp-D-Pro
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.15
-
cyclo-L-Trp-D-Pro
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.276
-
brevianamide F
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.489
-
dimethylallyl diphosphate
37°C, pH 7.5
Aspergillus versicolor
0.49
-
dimethylallyl diphosphate
pH 7.5, 37°C
Aspergillus versicolor
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Escherichia coli
Aspergillus versicolor
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.032
-
brevianamide F
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.082
-
cyclo-L-Trp-D-Pro
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.094
-
cyclo-L-Trp-L-Phe
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.098
-
dimethylallyl diphosphate
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.106
-
cyclo-L-Trp-L-Leu
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.139
-
cyclo-D-Trp-L-Ala
pH 7.5, 37°C
Aspergillus versicolor
0.214
-
cyclo-L-Trp-L-Tyr
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.709
-
cyclo-D-Trp-D-Pro
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.793
-
cyclo-L-Trp-D-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.942
-
cyclo-L-Trp-L-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
1.3
-
cyclo-L-Trp-Gly
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
1.318
-
cyclo-D-Trp-D-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
1.318
-
cyclo-D-Trp-L-Ala
37°C, pH 7.5
Aspergillus versicolor
2.906
-
cyclo-D-Trp-L-Pro
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
x * 45000, SDS-PAGE
Aspergillus versicolor
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
dimethylallyl diphosphate + brevianamide F
Aspergillus versicolor
-
diphosphate + deoxybrevianamide E
-
-
?
dimethylallyl diphosphate + brevianamide F
Aspergillus versicolor NRRL 573
-
diphosphate + deoxybrevianamide E
-
-
?
dimethylallyl diphosphate + brevianamide F
Aspergillus versicolor NRRL573
-
diphosphate + deoxybrevianamide E
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Aspergillus versicolor
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
dimethylallyl diphosphate + brevianamide F
-
721391
Aspergillus versicolor
diphosphate + deoxybrevianamide E
-
-
-
?
dimethylallyl diphosphate + brevianamide F
the enzyme accepts a broad range of tryptophan-containing cyclic dipeptides, no activity with farnesyl diphosphate and geranyl diphosphate
721391
Aspergillus versicolor
diphosphate + deoxybrevianamide E
-
-
-
?
dimethylallyl diphosphate + brevianamide F
i.e. cyclo-L-Trp-L-Pro
721391
Aspergillus versicolor
diphosphate + deoxybrevianamide E
-
-
-
?
dimethylallyl diphosphate + brevianamide F
-
721391
Aspergillus versicolor NRRL 573
diphosphate + deoxybrevianamide E
-
-
-
?
dimethylallyl diphosphate + brevianamide F
the enzyme accepts a broad range of tryptophan-containing cyclic dipeptides, no activity with farnesyl diphosphate and geranyl diphosphate
721391
Aspergillus versicolor NRRL 573
diphosphate + deoxybrevianamide E
-
-
-
?
dimethylallyl diphosphate + brevianamide F
i.e. cyclo-L-Trp-L-Pro
721391
Aspergillus versicolor NRRL 573
diphosphate + deoxybrevianamide E
-
-
-
?
dimethylallyl diphosphate + brevianamide F
-
721391
Aspergillus versicolor NRRL573
diphosphate + deoxybrevianamide E
-
-
-
?
dimethylallyl diphosphate + brevianamide F
the enzyme accepts a broad range of tryptophan-containing cyclic dipeptides, no activity with farnesyl diphosphate and geranyl diphosphate
721391
Aspergillus versicolor NRRL573
diphosphate + deoxybrevianamide E
-
-
-
?
dimethylallyl diphosphate + brevianamide F
i.e. cyclo-L-Trp-L-Pro
721391
Aspergillus versicolor NRRL573
diphosphate + deoxybrevianamide E
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-D-Ala
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-D-Ala
-
721391
Aspergillus versicolor NRRL 573
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-D-Ala
-
721391
Aspergillus versicolor NRRL573
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-D-Ala
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-D-Pro
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-D-Pro
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-L-Ala
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-L-Ala
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-L-Pro
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-D-Trp-L-Pro
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-D-Ala
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-D-Ala
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-D-Pro
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-D-Pro
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-Gly
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-Gly
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Ala
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Ala
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Leu
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Leu
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Phe
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Phe
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Tyr
-
721391
Aspergillus versicolor
diphosphate +
-
-
-
?
dimethylallyl diphosphate + cyclo-L-Trp-L-Tyr
-
721391
Aspergillus versicolor
diphosphate + ?
-
-
-
?
additional information
enzyme shows high flexibility towards its aromatic substrates and accepts all of the 14 tested tryptophan-containing cyclic dipeptides. The enzyme catalyzes the highly regiospecific reverse prenylation at C2 of the indole nucleus
721391
Aspergillus versicolor
?
-
-
-
-
additional information
enzyme shows high flexibility towards its aromatic substrates and accepts all of the 14 tested tryptophan-containing cyclic dipeptides. The enzyme catalyzes the highly regiospecific reverse prenylation at C2 of the indole nucleus
721391
Aspergillus versicolor NRRL 573
?
-
-
-
-
additional information
enzyme shows high flexibility towards its aromatic substrates and accepts all of the 14 tested tryptophan-containing cyclic dipeptides. The enzyme catalyzes the highly regiospecific reverse prenylation at C2 of the indole nucleus
721391
Aspergillus versicolor NRRL573
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 45000, SDS-PAGE
Aspergillus versicolor
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.003
-
cyclo-D-Trp-L-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.004
-
cyclo-L-Trp-L-Phe
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.006
-
cyclo-L-Trp-L-Tyr
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.011
-
cyclo-D-Trp-D-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.013
-
cyclo-L-Trp-D-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.014
-
cyclo-L-Trp-L-Leu
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.027
-
cyclo-D-Trp-L-Pro
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.032
-
cyclo-L-Trp-Gly
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.035
-
cyclo-L-Trp-L-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.095
-
cyclo-D-Trp-D-Pro
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.15
-
cyclo-L-Trp-D-Pro
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.276
-
brevianamide F
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.489
-
dimethylallyl diphosphate
37°C, pH 7.5
Aspergillus versicolor
0.49
-
dimethylallyl diphosphate
pH 7.5, 37°C
Aspergillus versicolor
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.009
-
cyclo-D-Trp-D-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.009
-
cyclo-D-Trp-L-Pro
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.017
-
cyclo-L-Trp-D-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.021
-
cyclo-D-Trp-L-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.025
-
cyclo-L-Trp-Gly
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.027
-
cyclo-L-Trp-L-Tyr
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.037
-
cyclo-L-Trp-L-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.04
-
cyclo-L-Trp-L-Phe
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.13
-
cyclo-D-Trp-D-Pro
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.134
-
cyclo-L-Trp-L-Leu
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
1.8
-
cyclo-L-Trp-D-Pro
pH 7.5, 37°C
Aspergillus versicolor
1.83
-
cyclo-L-Trp-D-Pro
37°C, pH 7.5
Aspergillus versicolor
4.99
-
dimethylallyl diphosphate
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
8.6
-
brevianamide F
pH 7.5, 37°C
Aspergillus versicolor
8.64
-
brevianamide F
37°C, pH 7.5
Aspergillus versicolor
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.009
-
cyclo-D-Trp-D-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.009
-
cyclo-D-Trp-L-Pro
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.017
-
cyclo-L-Trp-D-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.021
-
cyclo-D-Trp-L-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.025
-
cyclo-L-Trp-Gly
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.027
-
cyclo-L-Trp-L-Tyr
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.037
-
cyclo-L-Trp-L-Ala
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.04
-
cyclo-L-Trp-L-Phe
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.13
-
cyclo-D-Trp-D-Pro
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
0.134
-
cyclo-L-Trp-L-Leu
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
1.8
-
cyclo-L-Trp-D-Pro
pH 7.5, 37°C
Aspergillus versicolor
1.83
-
cyclo-L-Trp-D-Pro
37°C, pH 7.5
Aspergillus versicolor
4.99
-
dimethylallyl diphosphate
37°C, pH 7.5; pH 7.5, 37°C
Aspergillus versicolor
8.6
-
brevianamide F
pH 7.5, 37°C
Aspergillus versicolor
8.64
-
brevianamide F
37°C, pH 7.5
Aspergillus versicolor
Other publictions for EC 2.5.1.109
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737541
Wunsch
C7-prenylation of tryptophanyl ...
Aspergillus oryzae, Aspergillus terreus, Aspergillus terreus DSM 1958
Appl. Microbiol. Biotechnol.
99
1719-1730
2015
-
1
1
-
-
-
-
4
-
4
1
4
-
6
-
-
1
-
-
2
-
-
32
-
1
-
-
3
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
4
-
4
1
4
-
-
-
1
-
2
-
-
32
-
1
-
-
3
1
-
-
-
-
2
2
-
-
-
721391
Yin
Identification of a brevianami ...
Aspergillus versicolor, Aspergillus versicolor NRRL 573, Aspergillus versicolor NRRL573
Appl. Microbiol. Biotechnol.
97
1649-1660
2013
-
-
1
-
-
-
-
14
-
-
1
3
-
7
-
-
1
-
-
-
-
-
36
1
-
-
-
14
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
14
-
-
1
3
-
-
-
1
-
-
-
-
36
1
-
-
-
14
-
-
-
-
-
-
-
-
15
15
722446
Ding
Genome-based characterization ...
Aspergillus sp., Aspergillus sp. MF297-2
J. Am. Chem. Soc.
132
12733-12740
2010
-
-
1
-
6
-
4
2
-
3
2
2
-
2
-
-
-
-
-
-
-
-
4
1
1
1
-
2
1
1
-
-
-
-
-
-
-
1
-
-
6
-
-
4
-
2
-
3
2
2
-
-
-
-
-
-
-
-
4
1
1
1
-
2
1
1
-
-
-
-
-
-
2
2