Cloned (Comment) | Organism |
---|---|
- |
Aspergillus fumigatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus fumigatus | Q4WAW7 | - |
- |
Aspergillus fumigatus ATCC MYA-4609 | Q4WAW7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dimethylallyl diphosphate + brevianamide F | - |
Aspergillus fumigatus | diphosphate + (2S,3aR,8aR)-3a-(2-methylbut-3-en-2-yl)-1,2,3,3a,8,8a-hexahydropyrrolo[2,3-b]indole-2-carboxylic acid | product of mutant G115T, no product of wild-type | ? | |
dimethylallyl diphosphate + brevianamide F | - |
Aspergillus fumigatus ATCC MYA-4609 | diphosphate + (2S,3aR,8aR)-3a-(2-methylbut-3-en-2-yl)-1,2,3,3a,8,8a-hexahydropyrrolo[2,3-b]indole-2-carboxylic acid | product of mutant G115T, no product of wild-type | ? | |
additional information | the C5-C8 and C2-C9 reaction channels have very similar activation parameters for the prenylation step. Hence, the final proton ransfer/cyclization step is the product-determining step, with the deprotonation of the C5-C8 intermediate being nearly without activation (0.5 kcal/mol), while the deprotonation process for the C2-C9 pathway has a substantial barrier (higher than the C5-C8 and C2-C9 intermediate states) of 16.8 kcal/mol, resulting in the exclusive formation of the wild-type product tryprostatin B. The diphosphate anion leaving group is stabilized throughout by Arg113, Lys201, Tyr205, Gln380, Lys294, Tyr450, and nine crystal water molecules in the active site | Aspergillus fumigatus | ? | - |
? | |
additional information | the C5-C8 and C2-C9 reaction channels have very similar activation parameters for the prenylation step. Hence, the final proton ransfer/cyclization step is the product-determining step, with the deprotonation of the C5-C8 intermediate being nearly without activation (0.5 kcal/mol), while the deprotonation process for the C2-C9 pathway has a substantial barrier (higher than the C5-C8 and C2-C9 intermediate states) of 16.8 kcal/mol, resulting in the exclusive formation of the wild-type product tryprostatin B. The diphosphate anion leaving group is stabilized throughout by Arg113, Lys201, Tyr205, Gln380, Lys294, Tyr450, and nine crystal water molecules in the active site | Aspergillus fumigatus ATCC MYA-4609 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FtmPT1 | - |
Aspergillus fumigatus |