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Literature summary for 2.5.1.104 extracted from

  • Ohnuma, M.; Ganbe, T.; Terui, Y.; Niitsu, M.; Sato, T.; Tanaka, N.; Tamakoshi, M.; Samejima, K.; Kumasaka, T.; Oshima, T.
    Crystal structures and enzymatic properties of a triamine/agmatine aminopropyltransferase from Thermus thermophilus (2011), J. Mol. Biol., 408, 971-986.
    View publication on PubMed
Search for more literature for 2.5.1.104

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermus thermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor diffusion method Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00173
-
 spermidine pH 9.0, 37°C Thermus thermophilus
0.57
-
 norspermidine pH 9.0, 37°C Thermus thermophilus
0.77
-
 agmatine pH 9.0, 37°C Thermus thermophilus
1.64
-
 S-adenosyl 3-(methylthio)propylamine pH 9.0, 37°C Thermus thermophilus
38.31
-
 tris(3-aminopropyl)amine pH 9.0, 37°C Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36006
-
 4 * 36006, calculated from sequence Thermus thermophilus
130000
-
 gel filtration Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q5SK28
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information only faint activities (less than 10% of spermidine) are detected for diaminopropane [NH2(CH2)3NH2], putrescine, spermine, and caldopentamine Thermus thermophilus ?
-
 ?
S-adenosyl 3-(methylthio)propylamine + agmatine
-
 Thermus thermophilus S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
 ?
S-adenosyl 3-(methylthio)propylamine + homospermidine low activity Thermus thermophilus S-methyl-5'-thioadenosine + ?
-
 ?
S-adenosyl 3-(methylthio)propylamine + N,N'-bis(3-aminopropyl)-1,3-propanediamine i.e. thermine. Low activity Thermus thermophilus S-methyl-5'-thioadenosine + ?
-
 ?
S-adenosyl 3-(methylthio)propylamine + norspermidine
-
 Thermus thermophilus S-methyl-5'-thioadenosine + N,N'-bis(3-aminopropyl)-1,3-propanediamine i.e. thermine ?
S-adenosyl 3-(methylthio)propylamine + spermidine
-
 Thermus thermophilus S-methyl-5'-thioadenosine + spermine no formation of thermospermine (i.e. 1,12-diamino-4,8-diazadodecane) ?
S-adenosyl 3-(methylthio)propylamine + tris(3-aminopropyl)amine i.e. mitsubishine. Low activity. No formation of tetrakis(3-aminopropyl)ammonium Thermus thermophilus S-methyl-5'-thioadenosine + N4-aminopropylthermine
-
 ?

Subunits

Subunits Comment Organism
homotetramer 4 * 36006, calculated from sequence Thermus thermophilus

Synonyms

Synonyms Comment Organism
SpeE
-
 Thermus thermophilus
TAAPT
-
 Thermus thermophilus
triamine/agmatine aminopropyltransferase
-
 Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.29
-
 tris(3-aminopropyl)amine pH 9.0, 37°C Thermus thermophilus
0.37
-
 agmatine pH 9.0, 37°C Thermus thermophilus
0.53
-
 norspermidine pH 9.0, 37°C Thermus thermophilus
0.65
-
 spermidine pH 9.0, 37°C Thermus thermophilus
0.73
-
 S-adenosyl 3-(methylthio)propylamine pH 9.0, 37°C Thermus thermophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 at 60°C Thermus thermophilus
9
-
 assay at Thermus thermophilus
9.5
-
 at 37°C Thermus thermophilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
10
-
 stable around pH 10.0 at room temperature Thermus thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7.6
-
 tris(3-aminopropyl)amine pH 9.0, 37°C Thermus thermophilus
375
-
 spermidine pH 9.0, 37°C Thermus thermophilus
450
-
 S-adenosyl 3-(methylthio)propylamine pH 9.0, 37°C Thermus thermophilus
480
-
 agmatine pH 9.0, 37°C Thermus thermophilus
920
-
 norspermidine pH 9.0, 37°C Thermus thermophilus