Literature summary for 2.5.1.104 extracted from

  • Morimoto, N.; Fukuda, W.; Nakajima, N.; Masuda, T.; Terui, Y.; Kanai, T.; Oshima, T.; Imanaka, T.; Fujiwara, S.
    Dual biosynthesis pathway for longer-chain polyamines in the hyperthermophilic archaeon Thermococcus kodakarensis (2010), J. Bacteriol., 192, 4991-5001.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermococcus kodakarensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
agmatine pH 9.0, 70°C Thermococcus kodakarensis
1.053
-
putrescine pH 9.0, 70°C Thermococcus kodakarensis
0.0807
-
cadaverine pH 9.0, 70°C Thermococcus kodakarensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl 3-(methylthio)propylamine + agmatine Thermococcus kodakarensis the enzyme is involved in polyamine biosynthesis 5'-S-methyl-5'-thioadenosine + N1-aminopropylagamatine
-
?

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis Q5JFG9
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermococcus kodakarensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl 3-(methylthio)propylamine + agmatine the enzyme is involved in polyamine biosynthesis Thermococcus kodakarensis 5'-S-methyl-5'-thioadenosine + N1-aminopropylagamatine
-
?
S-adenosyl 3-(methylthio)propylamine + agmatine
-
Thermococcus kodakarensis 5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
?
S-adenosyl 3-(methylthio)propylamine + cadaverine
-
Thermococcus kodakarensis 5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine
-
?
S-adenosyl 3-(methylthio)propylamine + putrescine
-
Thermococcus kodakarensis 5'-S-methyl-5'-thioadenosine + spermidine
-
?

Synonyms

Synonyms Comment Organism
TK0147 protein
-
Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Thermococcus kodakarensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.3
-
putrescine pH 9.0, 70°C Thermococcus kodakarensis
0.28
-
agmatine pH 9.0, 70°C Thermococcus kodakarensis
0.02
-
cadaverine pH 9.0, 70°C Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
assay at Thermococcus kodakarensis

KCat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
22.5
-
agmatine pH 9.0, 70°C Thermococcus kodakarensis
0.29
-
putrescine pH 9.0, 70°C Thermococcus kodakarensis
0.29
-
cadaverine pH 9.0, 70°C Thermococcus kodakarensis