BRENDA - Enzyme Database
show all sequences of 2.5.1.104

The first agmatine/cadaverine aminopropyl transferase: biochemical and structural characterization of an enzyme involved in polyamine biosynthesis in the hyperthermophilic archaeon Pyrococcus furiosus

Cacciapuoti, G.; Porcelli, M.; Moretti, M.A.; Sorrentino, F.; Concilio, L.; Zappia, V.; Liu, Z.J.; Tempel, W.; Schubot, F.; Rose, J.P.; Wang, B.C.; Brereton, P.S.; Jenney, F.E.; Adams, M.W.; J. Bacteriol. 189, 6057-6067 (2007)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Pyrococcus furiosus
Crystallization (Commentary)
Crystallization (Commentary)
Organism
sitting drop vapor diffusion method, 1.8 A resolution
Pyrococcus furiosus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0076
-
agmatine
pH 7.4, 70°C
Pyrococcus furiosus
1.88
-
propane-1,3-diamine
pH 7.4, 70°C
Pyrococcus furiosus
3.77
-
cadaverine
pH 7.4, 70°C
Pyrococcus furiosus
7.7
-
putrescine
pH 7.4, 70°C
Pyrococcus furiosus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32334
-
2 * 32334, calculated from sequence
Pyrococcus furiosus
32600
-
2 * 32600, SDS-PAGE
Pyrococcus furiosus
65000
-
gel filtration
Pyrococcus furiosus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl 3-(methylthio)propylamine + agmatine
Pyrococcus furiosus
agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required
5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
-
?
S-adenosyl 3-(methylthio)propylamine + cadaverine
Pyrococcus furiosus
agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required
5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Pyrococcus furiosus
Q8U4G1
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Pyrococcus furiosus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
S-adenosyl 3-(methylthio)propylamine + agmatine
agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
-
-
?
S-adenosyl 3-(methylthio)propylamine + agmatine
4.9% of the activity compared to cadaverine
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
-
-
?
S-adenosyl 3-(methylthio)propylamine + cadaverine
-
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine
-
-
-
?
S-adenosyl 3-(methylthio)propylamine + cadaverine
agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine
-
-
-
?
S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine
2.9% of the activity compared to cadaverine
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine
-
-
-
?
S-adenosyl 3-(methylthio)propylamine + putrescine
8.7% of the activity compared to cadaverine
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + spermidine
-
-
-
?
S-adenosyl 3-(methylthio)propylamine + spermidine
0.8% of the activity compared to cadaverine
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + spermine
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 32334, calculated from sequence; 2 * 32600, SDS-PAGE
Pyrococcus furiosus
Synonyms
Synonyms
Commentary
Organism
ACAPT
-
Pyrococcus furiosus
agmatine/cadaverine aminopropyl transferase
-
Pyrococcus furiosus
PF0127
-
Pyrococcus furiosus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
70
-
assay at
Pyrococcus furiosus
90
-
-
Pyrococcus furiosus
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
70
103
70°C: about 50% of maximal activity, 103°C: about 50% of maximal activity
Pyrococcus furiosus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
108
-
transition temperature
Pyrococcus furiosus
112
-
transition temperature in presence of 5 mM cadaverine
Pyrococcus furiosus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.06
-
propane-1,3-diamine
pH 7.4, 70°C
Pyrococcus furiosus
0.1
-
agmatine
pH 7.4, 70°C
Pyrococcus furiosus
0.18
-
putrescine
pH 7.4, 70°C
Pyrococcus furiosus
2.09
-
cadaverine
pH 7.4, 70°C
Pyrococcus furiosus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Pyrococcus furiosus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pyrococcus furiosus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
sitting drop vapor diffusion method, 1.8 A resolution
Pyrococcus furiosus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0076
-
agmatine
pH 7.4, 70°C
Pyrococcus furiosus
1.88
-
propane-1,3-diamine
pH 7.4, 70°C
Pyrococcus furiosus
3.77
-
cadaverine
pH 7.4, 70°C
Pyrococcus furiosus
7.7
-
putrescine
pH 7.4, 70°C
Pyrococcus furiosus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32334
-
2 * 32334, calculated from sequence
Pyrococcus furiosus
32600
-
2 * 32600, SDS-PAGE
Pyrococcus furiosus
65000
-
gel filtration
Pyrococcus furiosus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl 3-(methylthio)propylamine + agmatine
Pyrococcus furiosus
agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required
5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
-
?
S-adenosyl 3-(methylthio)propylamine + cadaverine
Pyrococcus furiosus
agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required
5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pyrococcus furiosus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
S-adenosyl 3-(methylthio)propylamine + agmatine
agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
-
-
?
S-adenosyl 3-(methylthio)propylamine + agmatine
4.9% of the activity compared to cadaverine
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
-
-
?
S-adenosyl 3-(methylthio)propylamine + cadaverine
-
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine
-
-
-
?
S-adenosyl 3-(methylthio)propylamine + cadaverine
agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine
-
-
-
?
S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine
2.9% of the activity compared to cadaverine
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine
-
-
-
?
S-adenosyl 3-(methylthio)propylamine + putrescine
8.7% of the activity compared to cadaverine
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + spermidine
-
-
-
?
S-adenosyl 3-(methylthio)propylamine + spermidine
0.8% of the activity compared to cadaverine
719710
Pyrococcus furiosus
5'-S-methyl-5'-thioadenosine + spermine
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 32334, calculated from sequence; 2 * 32600, SDS-PAGE
Pyrococcus furiosus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
70
-
assay at
Pyrococcus furiosus
90
-
-
Pyrococcus furiosus
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
70
103
70°C: about 50% of maximal activity, 103°C: about 50% of maximal activity
Pyrococcus furiosus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
108
-
transition temperature
Pyrococcus furiosus
112
-
transition temperature in presence of 5 mM cadaverine
Pyrococcus furiosus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.06
-
propane-1,3-diamine
pH 7.4, 70°C
Pyrococcus furiosus
0.1
-
agmatine
pH 7.4, 70°C
Pyrococcus furiosus
0.18
-
putrescine
pH 7.4, 70°C
Pyrococcus furiosus
2.09
-
cadaverine
pH 7.4, 70°C
Pyrococcus furiosus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Pyrococcus furiosus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.023
-
putrescine
pH 7.4, 70°C
Pyrococcus furiosus
0.032
-
propane-1,3-diamine
pH 7.4, 70°C
Pyrococcus furiosus
0.55
-
cadaverine
pH 7.4, 70°C
Pyrococcus furiosus
13.4
-
agmatine
pH 7.4, 70°C
Pyrococcus furiosus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.023
-
putrescine
pH 7.4, 70°C
Pyrococcus furiosus
0.032
-
propane-1,3-diamine
pH 7.4, 70°C
Pyrococcus furiosus
0.55
-
cadaverine
pH 7.4, 70°C
Pyrococcus furiosus
13.4
-
agmatine
pH 7.4, 70°C
Pyrococcus furiosus
Other publictions for EC 2.5.1.104
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
720250
Ohnuma
Crystal structures and enzymat ...
Thermus thermophilus
J. Mol. Biol.
408
971-986
2011
-
-
1
1
-
-
-
5
-
-
2
-
-
3
-
-
1
-
-
-
-
-
7
1
3
1
-
-
5
3
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
5
-
-
2
-
-
-
-
1
-
-
-
-
7
1
1
-
-
5
3
-
1
-
-
-
-
-
5
5
719718
Morimoto
Dual biosynthesis pathway for ...
Thermococcus kodakarensis
J. Bacteriol.
192
4991-5001
2010
-
-
1
-
-
-
-
3
-
-
-
1
-
1
-
-
1
-
-
-
-
-
4
-
1
1
-
-
3
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
3
-
-
-
1
-
-
-
1
-
-
-
-
4
-
1
-
-
3
1
-
-
-
-
-
-
-
3
3
719710
Cacciapuoti
The first agmatine/cadaverine ...
Pyrococcus furiosus
J. Bacteriol.
189
6057-6067
2007
-
-
1
1
-
-
-
4
-
-
3
2
-
5
-
-
1
-
-
-
-
-
7
1
3
2
1
2
4
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
4
-
-
3
2
-
-
-
1
-
-
-
-
7
1
2
1
2
4
1
-
-
-
-
-
-
-
4
4
719832
Ohnuma
N1-aminopropylagmatine, a new ...
Thermus thermophilus
J. Biol. Chem.
280
30073-30082
2005
-
-
1
-
-
-
-
1
-
-
1
1
-
1
-
-
1
-
-
-
1
-
2
1
2
1
-
-
1
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
-
-
1
1
-
-
-
1
-
-
1
-
2
1
1
-
-
1
1
-
-
-
-
-
-
-
1
1