Literature summary for 2.5.1.104 extracted from

  • Cacciapuoti, G.; Porcelli, M.; Moretti, M.A.; Sorrentino, F.; Concilio, L.; Zappia, V.; Liu, Z.J.; Tempel, W.; Schubot, F.; Rose, J.P.; Wang, B.C.; Brereton, P.S.; Jenney, F.E.; Adams, M.W.
    The first agmatine/cadaverine aminopropyl transferase: biochemical and structural characterization of an enzyme involved in polyamine biosynthesis in the hyperthermophilic archaeon Pyrococcus furiosus (2007), J. Bacteriol., 189, 6057-6067.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus furiosus

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, 1.8 A resolution Pyrococcus furiosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0076
-
agmatine pH 7.4, 70°C Pyrococcus furiosus
7.7
-
putrescine pH 7.4, 70°C Pyrococcus furiosus
1.88
-
propane-1,3-diamine pH 7.4, 70°C Pyrococcus furiosus
3.77
-
cadaverine pH 7.4, 70°C Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
gel filtration Pyrococcus furiosus
32600
-
2 * 32600, SDS-PAGE Pyrococcus furiosus
32334
-
2 * 32334, calculated from sequence Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl 3-(methylthio)propylamine + agmatine Pyrococcus furiosus agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required 5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
?
S-adenosyl 3-(methylthio)propylamine + cadaverine Pyrococcus furiosus agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required 5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U4G1
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl 3-(methylthio)propylamine + agmatine agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required Pyrococcus furiosus 5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
?
S-adenosyl 3-(methylthio)propylamine + agmatine 4.9% of the activity compared to cadaverine Pyrococcus furiosus 5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
?
S-adenosyl 3-(methylthio)propylamine + cadaverine
-
Pyrococcus furiosus 5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine
-
?
S-adenosyl 3-(methylthio)propylamine + cadaverine agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required Pyrococcus furiosus 5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine
-
?
S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine 2.9% of the activity compared to cadaverine Pyrococcus furiosus 5'-S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine
-
?
S-adenosyl 3-(methylthio)propylamine + putrescine 8.7% of the activity compared to cadaverine Pyrococcus furiosus 5'-S-methyl-5'-thioadenosine + spermidine
-
?
S-adenosyl 3-(methylthio)propylamine + spermidine 0.8% of the activity compared to cadaverine Pyrococcus furiosus 5'-S-methyl-5'-thioadenosine + spermine
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 32600, SDS-PAGE Pyrococcus furiosus
homodimer 2 * 32334, calculated from sequence Pyrococcus furiosus

Synonyms

Synonyms Comment Organism
agmatine/cadaverine aminopropyl transferase
-
Pyrococcus furiosus
ACAPT
-
Pyrococcus furiosus
PF0127
-
Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
assay at Pyrococcus furiosus
90
-
-
Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 103 70°C: about 50% of maximal activity, 103°C: about 50% of maximal activity Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
112
-
transition temperature in presence of 5 mM cadaverine Pyrococcus furiosus
108
-
transition temperature Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
agmatine pH 7.4, 70°C Pyrococcus furiosus
0.06
-
propane-1,3-diamine pH 7.4, 70°C Pyrococcus furiosus
0.18
-
putrescine pH 7.4, 70°C Pyrococcus furiosus
2.09
-
cadaverine pH 7.4, 70°C Pyrococcus furiosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Pyrococcus furiosus

KCat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.032
-
propane-1,3-diamine pH 7.4, 70°C Pyrococcus furiosus
0.55
-
cadaverine pH 7.4, 70°C Pyrococcus furiosus
0.023
-
putrescine pH 7.4, 70°C Pyrococcus furiosus
13.4
-
agmatine pH 7.4, 70°C Pyrococcus furiosus