BRENDA - Enzyme Database
show all sequences of 2.5.1.101

The CMP-legionaminic acid pathway in Campylobacter: Biosynthesis involving novel GDP-linked precursors

Schoenhofen, I.; Vinogradov, E.; Whitfield, D.; Brisson, J.; Logan, S.; Glycobiology 19, 715-725 (2009)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene legI or Cj1327, DNA and ammino acid sequence determination and analysis, expression as N-terminally His6-tagged enzyme
Campylobacter jejuni
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
Campylobacter jejuni
-
N,N'-diacetyllegionaminate + phosphate
-
-
?
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
Campylobacter jejuni 11168
-
N,N'-diacetyllegionaminate + phosphate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Campylobacter jejuni
-
gene legI or Cj1327
-
Campylobacter jejuni 11168
-
gene legI or Cj1327
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant N-terminally His6-tagged enzyme by nickel affinity chromatography
Campylobacter jejuni
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
-
719563
Campylobacter jejuni
N,N'-diacetyllegionaminate + phosphate
-
-
-
?
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
-
719563
Campylobacter jejuni 11168
N,N'-diacetyllegionaminate + phosphate
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
legI
-
Campylobacter jejuni
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Campylobacter jejuni
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
assay at
Campylobacter jejuni
Cloned(Commentary) (protein specific)
Commentary
Organism
gene legI or Cj1327, DNA and ammino acid sequence determination and analysis, expression as N-terminally His6-tagged enzyme
Campylobacter jejuni
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
Campylobacter jejuni
-
N,N'-diacetyllegionaminate + phosphate
-
-
?
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
Campylobacter jejuni 11168
-
N,N'-diacetyllegionaminate + phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant N-terminally His6-tagged enzyme by nickel affinity chromatography
Campylobacter jejuni
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
-
719563
Campylobacter jejuni
N,N'-diacetyllegionaminate + phosphate
-
-
-
?
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
-
719563
Campylobacter jejuni 11168
N,N'-diacetyllegionaminate + phosphate
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Campylobacter jejuni
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.3
-
assay at
Campylobacter jejuni
General Information
General Information
Commentary
Organism
metabolism
the enzyme is part of the biosynthesis of legionaminic acid and its CMP-activated form, metabolite determination and identification by NMR spectroscoopy for pathway construction and analysis, overview
Campylobacter jejuni
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is part of the biosynthesis of legionaminic acid and its CMP-activated form, metabolite determination and identification by NMR spectroscoopy for pathway construction and analysis, overview
Campylobacter jejuni
Other publictions for EC 2.5.1.101
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719563
Schoenhofen
The CMP-legionaminic acid path ...
Campylobacter jejuni, Campylobacter jejuni 11168
Glycobiology
19
715-725
2009
-
-
1
-
-
-
-
-
-
-
-
2
-
5
-
-
1
-
-
-
-
-
2
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
718850
Glaze
Biosynthesis of CMP-N,N-diacet ...
Legionella pneumophila, Legionella pneumophila ATCC 33152D
Biochemistry
47
3272-3282
2008
-
-
1
-
-
-
-
-
-
1
-
4
-
4
-
-
1
1
-
-
-
-
8
-
3
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
1
-
-
-
-
8
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
718948
Knirel
Identification of a homopolyme ...
Legionella pneumophila
Biochemistry
66
1035-1041
2001
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-