BRENDA - Enzyme Database
show all sequences of 2.5.1.101

Identification of a homopolymer of 5-acetamidino-7-acetamido-3,5,7,9-tetradeoxy-D-glycero-D-talo-nonulosonic acid in the lipopolysaccharides of Legionella pneumophila non-1 serogroups

Knirel, Y.; Senchenkova, S.; Kocharova, N.; Shashkov, A.; Helbig, J.; Zaehringer, U.; Biochemistry 66, 1035-1041 (2001)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene lpg 0752, cloning and expression of NeuB results in insoluble protein which possesses very low levels of N,N'-diacetyllegionaminate synthase activity
Legionella pneumophila
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
Legionella pneumophila
-
N,N'-diacetyllegionaminate + phosphate
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Legionella pneumophila
-
serogroups 2-14, and strains Lansing 3 and 16453-92 from another serogroup, gene lpg 0752 or neuB homologue
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
-
718948
Legionella pneumophila
N,N'-diacetyllegionaminate + phosphate
-
-
-
?
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
-
718948
Legionella pneumophila
N,N'-diacetyllegionaminate + phosphate
i.e. Leg5Ac7Ac
-
-
?
Synonyms
Synonyms
Commentary
Organism
NeuB
-
Legionella pneumophila
nonulosonic acid synthase
-
Legionella pneumophila
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Legionella pneumophila
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Legionella pneumophila
Cloned(Commentary) (protein specific)
Commentary
Organism
gene lpg 0752, cloning and expression of NeuB results in insoluble protein which possesses very low levels of N,N'-diacetyllegionaminate synthase activity
Legionella pneumophila
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
Legionella pneumophila
-
N,N'-diacetyllegionaminate + phosphate
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
-
718948
Legionella pneumophila
N,N'-diacetyllegionaminate + phosphate
-
-
-
?
2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose + phosphoenolpyruvate + H2O
-
718948
Legionella pneumophila
N,N'-diacetyllegionaminate + phosphate
i.e. Leg5Ac7Ac
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Legionella pneumophila
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Legionella pneumophila
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in the the Leg pathway, which is essential for the biosynthesis of CMP-N,N'-diacetyllegionaminate, that acts as a donor substrate with a selection of bacterial and mammalian sialyltransferases
Legionella pneumophila
additional information
NeuB can be substituted by NanA from Escherichia coli, a sialic acid aldolase that catalyzes the condensation of 2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose with pyruvate resulting in N,N'-diacetyllegionaminate formation, ability of sialyltransferases to utilize the CMP-N,N'-diacetyllegionaminate donor, overview
Legionella pneumophila
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in the the Leg pathway, which is essential for the biosynthesis of CMP-N,N'-diacetyllegionaminate, that acts as a donor substrate with a selection of bacterial and mammalian sialyltransferases
Legionella pneumophila
additional information
NeuB can be substituted by NanA from Escherichia coli, a sialic acid aldolase that catalyzes the condensation of 2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose with pyruvate resulting in N,N'-diacetyllegionaminate formation, ability of sialyltransferases to utilize the CMP-N,N'-diacetyllegionaminate donor, overview
Legionella pneumophila
Other publictions for EC 2.5.1.101
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719563
Schoenhofen
The CMP-legionaminic acid path ...
Campylobacter jejuni, Campylobacter jejuni 11168
Glycobiology
19
715-725
2009
-
-
1
-
-
-
-
-
-
-
-
2
-
5
-
-
1
-
-
-
-
-
2
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
718850
Glaze
Biosynthesis of CMP-N,N-diacet ...
Legionella pneumophila, Legionella pneumophila ATCC 33152D
Biochemistry
47
3272-3282
2008
-
-
1
-
-
-
-
-
-
1
-
4
-
4
-
-
1
1
-
-
-
-
8
-
3
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
1
-
-
-
-
8
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
718948
Knirel
Identification of a homopolyme ...
Legionella pneumophila
Biochemistry
66
1035-1041
2001
-
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-