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Literature summary for 2.5.1.10 extracted from
- Increasing the intracellular isoprenoid pool in Saccharomyces cerevisiae by structural fine-tuning of a bifunctional farnesyl diphosphate synthase (2017), FEMS Yeast Res., 17, fox032 .
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A99G | mutant enzyme shows a significant increase in the total concentration of isoprene: 2.4times compared to the wild-type strain. No detection of geranyl diphosphate | Saccharomyces cerevisiae |
| A99H | the strain carrying the variant shows significant differences on geranyl diphosphate concentrations and specific growth rate | Saccharomyces cerevisiae |
| A99H | variant shows significant difference on geranyl diphosphate concentrations and specific growth rate | Saccharomyces cerevisiae |
| A99K | no detection of geranyl diphosphate | Saccharomyces cerevisiae |
| A99K | the strain carrying the mutant enzyme accumulates high amounts of dimethylallyl diphosphate-isopentenyl diphosphate, with a decrease in geranyl diphosphate and farnesyl diphosphate | Saccharomyces cerevisiae |
| A99P | mutant enzyme shows a significant increase in the total concentration of isoprene: 6.0times compared to the wild-type strain. The mutant enzyme shows a significant accumulation of isoprene phosphate particularly of isopentenyl diphosphate and/or dimethylallyl diphosphate, which are not detected in the wild-type strain. 1.5fold increase in geranyl diphosphate concentration compared to wild-type enzyme | Saccharomyces cerevisiae |
| A99Q | mutant enzyme shows a significant increase in the total concentration of isoprene: 8.0times compared to the wild-type strain. The mutant enzyme shows a significant accumulation of isoprene phosphate particularly of isopentenyl diphosphate and/or dimethylallyl diphosphate, which are not detected in the wild-type strain. No detection of geranyl diphosphate | Saccharomyces cerevisiae |
| A99Q | the strain carrying the mutant enzyme accumulates high amounts of dimethylallyl diphosphate-isopentenyl diphosphate, with a decrease in geranyl diphosphate and farnesyl diphosphate | Saccharomyces cerevisiae |
| A99R | the strain carrying the variant shows significant differences on geranyl diphosphate concentrations and specific growth rate | Saccharomyces cerevisiae |
| A99R | variant shows significant difference on geranyl diphosphate concentrations and specific growth rate | Saccharomyces cerevisiae |
| A99T | mutant enzyme behaves similarly to the wild-type strain with respect to the geranyl diphosphate content | Saccharomyces cerevisiae |
| A99T | the mutant produces unquantifiable amounts of farnesyl diphosphate and no effect on geranyl diphosphate production is observed | Saccharomyces cerevisiae |
| A99T | the strain carrying the variant produces unquantifiable amounts of farnesyl diphosphate and no effect on geranyl diphosphate production is observed | Saccharomyces cerevisiae |
| A99V | mutant enzyme behaves similarly to the wild-type strain with respect to the geranyl diphosphate content | Saccharomyces cerevisiae |
| A99W | mutant enzyme shows a significant increase in the total concentration of isoprene: 2.1times compared to the wild-type strain. 3fold increase in geranyl diphosphate concentration compared to wild-type enzyme | Saccharomyces cerevisiae |
| A99Y | mutant enzyme shows a significant accumulation of isoprene phosphate particularly of isopentenyl diphosphate and/or dimethylallyl diphosphate, which are not detected in the wild-type strain | Saccharomyces cerevisiae |
| A99Y | mutant enzyme shows a significant increase in the total concentration of isoprene: 3.1times compared to the wild-type strain. The mutant enzyme shows a significant accumulation of isoprene phosphate particularly of isopentenyl diphosphate and/or dimethylallyl diphosphate, which are not detected in the wild-type strain. No detection of geranyl diphosphate | Saccharomyces cerevisiae |
| A99Y | the strain carrying the mutant enzyme accumulates high amounts of dimethylallyl diphosphate-isopentenyl diphosphate, with a decrease in geranyl diphosphate and farnesyl diphosphate | Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + isopentenyl diphosphate | Saccharomyces cerevisiae | reaction of EC 2.5.1.1. the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate | diphosphate + geranyl diphosphate | - |
? |  |
| dimethylallyl diphosphate + isopentenyl diphosphate | Saccharomyces cerevisiae | the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate, producing geranyl diphosphate and farnesyl diphosphate | diphosphate + geranyl diphosphate | - |
? | |
| dimethylallyl diphosphate + isopentenyl diphosphate | Saccharomyces cerevisiae BY4741 | reaction of EC 2.5.1.1. the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate | diphosphate + geranyl diphosphate | - |
? | |
| dimethylallyl diphosphate + isopentenyl diphosphate | Saccharomyces cerevisiae BY4741 | the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate, producing geranyl diphosphate and farnesyl diphosphate | diphosphate + geranyl diphosphate | - |
? | |
| geranyl diphosphate + isopentenyl diphosphate | Saccharomyces cerevisiae | the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate | diphosphate + (2E,6E)-farnesyl diphosphate | - |
? | |
| geranyl diphosphate + isopentenyl diphosphate | Saccharomyces cerevisiae | the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate, producing geranyl diphosphate and farnesyl diphosphate | diphosphate + (2E,6E)-farnesyl diphosphate | - |
? | |
| geranyl diphosphate + isopentenyl diphosphate | Saccharomyces cerevisiae BY4741 | the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate | diphosphate + (2E,6E)-farnesyl diphosphate | - |
? | |
| geranyl diphosphate + isopentenyl diphosphate | Saccharomyces cerevisiae BY4741 | the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate, producing geranyl diphosphate and farnesyl diphosphate | diphosphate + (2E,6E)-farnesyl diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | P08524 | S288c-derivative laboratory strain | - |
| Saccharomyces cerevisiae BY4741 | P08524 | S288c-derivative laboratory strain | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dimethylallyl diphosphate + isopentenyl diphosphate | reaction of EC 2.5.1.1. the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate | Saccharomyces cerevisiae | diphosphate + geranyl diphosphate | - |
? | |
| dimethylallyl diphosphate + isopentenyl diphosphate | the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate, producing geranyl diphosphate and farnesyl diphosphate | Saccharomyces cerevisiae | diphosphate + geranyl diphosphate | - |
? | |
| dimethylallyl diphosphate + isopentenyl diphosphate | reaction of EC 2.5.1.1. the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate | Saccharomyces cerevisiae BY4741 | diphosphate + geranyl diphosphate | - |
? | |
| dimethylallyl diphosphate + isopentenyl diphosphate | the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate, producing geranyl diphosphate and farnesyl diphosphate | Saccharomyces cerevisiae BY4741 | diphosphate + geranyl diphosphate | - |
? | |
| geranyl diphosphate + isopentenyl diphosphate | the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate | Saccharomyces cerevisiae | diphosphate + (2E,6E)-farnesyl diphosphate | - |
? | |
| geranyl diphosphate + isopentenyl diphosphate | the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate, producing geranyl diphosphate and farnesyl diphosphate | Saccharomyces cerevisiae | diphosphate + (2E,6E)-farnesyl diphosphate | - |
? | |
| geranyl diphosphate + isopentenyl diphosphate | the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate (IPP) with dimethylallyl diphosphate (DMAPP), producing geranyl diphosphate (GPP) and farnesyl diphosphate | Saccharomyces cerevisiae BY4741 | diphosphate + (2E,6E)-farnesyl diphosphate | - |
? | |
| geranyl diphosphate + isopentenyl diphosphate | the enzyme catalyzes the sequential condensation of two molecules of isopentenyl diphosphate with dimethylallyl diphosphate, producing geranyl diphosphate and farnesyl diphosphate | Saccharomyces cerevisiae BY4741 | diphosphate + (2E,6E)-farnesyl diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ERG20 | - |
Saccharomyces cerevisiae |
| FPPS | - |
Saccharomyces cerevisiae |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | farnesyl diphosphate synthase is a key enzyme responsible for the supply of isoprenoid precursors for several essential metabolites, including sterols, dolichols and ubiquinone | Saccharomyces cerevisiae |
| physiological function | key enzyme responsible for the supply of isoprenoid precursors for several essential metabolites, including sterols, dolichols and ubiquinone | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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