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Literature summary for 2.5.1.10 extracted from

  • Zhao, Y.J.; Chen, X.; Zhang, M.; Su, P.; Liu, Y.J.; Tong, Y.R.; Wang, X.J.; Huang, L.Q.; Gao, W.
    Molecular cloning and characterisation of farnesyl pyrophosphate synthase from Tripterygium wilfordii (2015), PLoS ONE, 10, e0125415.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.10

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Tripterygium wilfordii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
-
 Tripterygium wilfordii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranyl diphosphate + isopentenyl diphosphate Tripterygium wilfordii
-
 diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Tripterygium wilfordii A0A0F6PMF9
-
-
Tripterygium wilfordii A0A0F6PN04
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Tripterygium wilfordii

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Tripterygium wilfordii
-
root high expression Tripterygium wilfordii
-
stem
-
 Tripterygium wilfordii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranyl diphosphate + isopentenyl diphosphate
-
 Tripterygium wilfordii diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 39540, calculated from amino acid sequence Tripterygium wilfordii
? x * 58000, enzyme fused with a Trx-tag and a His-tag on N-terminal Tripterygium wilfordii

Synonyms

Synonyms Comment Organism
farnesyl pyrophosphate synthase
-
 Tripterygium wilfordii
Fps1 isoform Tripterygium wilfordii
FPS2 isoform Tripterygium wilfordii

pI Value

Organism Comment pI Value Maximum pI Value
Tripterygium wilfordii isoform FPS2, calculated from amino acid sequence
-
 5.3
Tripterygium wilfordii isoform FPS1, calculated from amino acid sequence
-
 5.6

Expression

Organism Comment Expression
Tripterygium wilfordii the enzyme expression is up-regulated upon 1 mM methyl jasmonate treatment after 12 h up