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Literature summary for 2.5.1.10 extracted from

  • Aripirala, S.; Gonzalez-Pacanowska, D.; Oldfield, E.; Kaiser, M.; Amzel, L.M.; Gabelli, S.B.
    Structural and thermodynamic basis of the inhibition of Leishmania major farnesyl diphosphate synthase by nitrogen-containing bisphosphonates (2014), Acta Crystallogr. Sect. D, 70, 802-810.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.5.1.10

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with isopentenyl diphosphate and inhibitors, hanging drop vapor diffusion method, using 15-25% (w/v) PEG 3350, 0.1-0.2 M calcium acetate, 0.1 M MES sodium salt pH 6.5 Leishmania major

Inhibitors

Inhibitors Comment Organism Structure
1-(2-hydroxy-2,2-diphosphonoethyl)-3-phenylpyridinium
-
 Leishmania major
3-butyl-1-(2,2-diphosphonoethyl)pyridinium
-
 Leishmania major
3-fluoro-1-(2-hydroxy-2,2-diphosphonoethyl)-pyridinium
-
 Leishmania major
zoledronate
-
 Leishmania major

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ contains Mg2+ Leishmania major

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
geranyl diphosphate + isopentenyl diphosphate Leishmania major
-
 diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Leishmania major
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap Ni2+-chelate column chromatography Leishmania major

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
geranyl diphosphate + isopentenyl diphosphate
-
 Leishmania major diphosphate + (2E,6E)-farnesyl diphosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Leishmania major

Synonyms

Synonyms Comment Organism
farnesyl pyrophosphate synthase
-
 Leishmania major
FPPS
-
 Leishmania major

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000001
-
 1-(2-hydroxy-2,2-diphosphonoethyl)-3-phenylpyridinium pH and temperature not specified in the publication Leishmania major
0.000011
-
 zoledronate pH and temperature not specified in the publication Leishmania major
0.00005
-
 3-fluoro-1-(2-hydroxy-2,2-diphosphonoethyl)-pyridinium pH and temperature not specified in the publication Leishmania major