Literature summary for 2.5.1.10 extracted from

Nagaki, M.; Ichijo, T.; Kobashi, R.; Yagihashi, Y.; Musashi, T.; Kawakami, J.; Ohya, N.; Gotoh, T.; Sagami, H.
Substrate specificities of E- and Z-farnesyl diphosphate synthases with substrate analogs (2012), J. Mol. Catal. B, 80, 1-6.

No PubMed abstract available

Search for more literature for 2.5.1.10

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli DH5alpha cells	Geobacillus stearothermophilus

Protein Variants

Protein Variants	Comment	Organism
Y81S	the mutant catalyzes the reaction between 8-tetrahydropyran-2-yloxygeranyl diphosphate and isopentenyl diphosphate, producing 12-tetrahydropyran-2-yloxyfarnesyl diphosphate with a yield of 12.3%. The mutant catalyzes the reaction between 8-acetoxygeranyl diphosphate with isopentenyl diphosphate, producing 12-acetoxyfarnesyl diphosphate and 16-acetoxygeranylgeranyl diphosphate with respective yields of 55.3% and 1.7%	Geobacillus stearothermophilus

Organism

Organism	UniProt	Comment	Textmining
Geobacillus stearothermophilus	Q08291	-	-
Geobacillus stearothermophilus ATCC 10149	Q08291	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
8-acetoxygeranyl diphosphate + isopentenyl diphosphate	-	Geobacillus stearothermophilus	diphosphate + 12-acetoxyfarnesyl diphosphate	21.8% yield	?
8-acetoxygeranyl diphosphate + isopentenyl diphosphate	-	Geobacillus stearothermophilus ATCC 10149	diphosphate + 12-acetoxyfarnesyl diphosphate	21.8% yield	?
geranyl diphosphate + isopentenyl diphosphate	-	Geobacillus stearothermophilus	diphosphate + (2E,6E)-farnesyl diphosphate	-	?
geranyl diphosphate + isopentenyl diphosphate	-	Geobacillus stearothermophilus ATCC 10149	diphosphate + (2E,6E)-farnesyl diphosphate	-	?

Synonyms

Synonyms	Comment	Organism
E-farnesyl diphosphate synthase	-	Geobacillus stearothermophilus
E-FPP synthase	-	Geobacillus stearothermophilus

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Release 2023.1 (January 2023)