Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Carbonate | 100% activity in the presence of 25 mM carbonate | Escherichia coli | |
DNA | activity is highest in the presence of 0.2 mg/ml DNA | Escherichia coli | |
EDTA | 100% activity in the presence of 100 mM EDTA | Escherichia coli | |
Sodium acetate | 100% activity in the presence of 750 mM sodium acetate | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5apha cells | Escherichia coli |
General Stability | Organism |
---|---|
replacing Tris buffer with glycyclglycine increases activity 2fold | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 43% residual activity at 10 mM | Escherichia coli | |
KCl | 28% residual activity at 100 mM | Escherichia coli | |
Mg2+ | Mg2+ in millimolar concentrations inhibits enzyme activity but has little or no effect in the submillimolar range | Escherichia coli | |
Mn2+ | Mn2+ in millimolar concentrations inhibits enzyme activity but has little or no effect in the submillimolar range | Escherichia coli | |
Na2PO4 | 80% residual activity at 25 mM | Escherichia coli | |
Na2SO4 | 33% residual activity at 25 mM | Escherichia coli | |
NaCl | 25% residual activity at 100 mM | Escherichia coli | |
NaSCN | 60% residual activity at 0.01 mM | Escherichia coli | |
potassium fluoride | 78% residual activity at 500 mM | Escherichia coli | |
Tris-HCl | 11% residual activity at 100 mM | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
7-aminomethyl-7-deazaguanine in tRNA(Tyr) | in 100 mM glycylglycine (pH 8.7), 100 mM EDTA, 100 mM KCl, 0.5 mM dithiothreitol, at 37°C | Escherichia coli | |
0.0377 | - |
7-aminomethyl-7-deazaguanine in minihelix RNA | in 100 mM glycylglycine (pH 8.7), 100 mM EDTA, 100 mM KCl, 0.5 mM dithiothreitol, at 37°C | Escherichia coli | |
0.1014 | - |
S-adenosyl-L-methionine | in 100 mM glycylglycine (pH 8.7), 100 mM EDTA, 100 mM KCl, 0.5 mM dithiothreitol, at 37°C | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 7-aminomethyl-7-deazaguanine in tRNA(Tyr) | Escherichia coli | - |
L-methionine + adenine + epoxyqueuosine in tRNA(Tyr) | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose column chromatography and POROS HQ column chromatography | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + 7-aminomethyl-7-deazaguanine in minihelix RNA | efficient substrate | Escherichia coli | L-methionine + adenine + epoxyqueuosine in minihelix RNA | - |
ir | |
S-adenosyl-L-methionine + 7-aminomethyl-7-deazaguanine in tRNA(Tyr) | - |
Escherichia coli | L-methionine + adenine + epoxyqueuosine in tRNA(Tyr) | - |
ir |
Synonyms | Comment | Organism |
---|---|---|
QueA | - |
Escherichia coli |
S-adenosylmethionine:tRNA ribosyltransferase-isomerase | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
S-adenosyl-L-methionine | in 100 mM glycylglycine (pH 8.7), 100 mM EDTA, 100 mM KCl, 0.5 mM dithiothreitol, at 37°C | Escherichia coli | |
0.042 | - |
7-aminomethyl-7-deazaguanine in tRNA(Tyr) | in 100 mM glycylglycine (pH 8.7), 100 mM EDTA, 100 mM KCl, 0.5 mM dithiothreitol, at 37°C | Escherichia coli | |
0.245 | - |
7-aminomethyl-7-deazaguanine in minihelix RNA | in 100 mM glycylglycine (pH 8.7), 100 mM EDTA, 100 mM KCl, 0.5 mM dithiothreitol, at 37°C | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.7 | - |
optimal activity at pH 8.7 in buffers containing various oxyanions, including acetate, carbonate, EDTA, and phosphate | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme catalyzes the penultimate step in the de novo biosynthesis of queuosine | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.67 | - |
7-aminomethyl-7-deazaguanine in minihelix RNA | in 100 mM glycylglycine (pH 8.7), 100 mM EDTA, 100 mM KCl, 0.5 mM dithiothreitol, at 37°C | Escherichia coli | |
28.3 | - |
7-aminomethyl-7-deazaguanine in tRNA(Tyr) | in 100 mM glycylglycine (pH 8.7), 100 mM EDTA, 100 mM KCl, 0.5 mM dithiothreitol, at 37°C | Escherichia coli |