Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.99.17 extracted from

  • Van Lanen, S.G.; Iwata-Reuyl, D.
    Kinetic mechanism of the tRNA-modifying enzyme S-adenosylmethionine:tRNA ribosyltransferase-isomerase (QueA) (2003), Biochemistry, 42, 5312-5320.
    View publication on PubMed
Search for more literature for 2.4.99.17

Inhibitors

Inhibitors Comment Organism Structure
adenine noncompetitive inhibition against S-adenosyl-L-methionine and 7-(aminomethyl)-7-deazaguanine-tRNATyr Escherichia coli
epoxyqueuosine-tRNATyr competitive inhibition against 7-(aminomethyl)-7-deazaguanine-tRNATyr and noncompetitive inhibition against S-adenosyl-L-methionine Escherichia coli
L-methionine uncompetitive inhibition versus 7-(aminomethyl)-7-deazaguanine-tRNATyr and noncompetitive inhibition against S-adenosyl-L-methionine Escherichia coli
S-adenosylhomocysteine competitive inhibition against S-adenosyl-L-methionine and noncompetitive inhibition against 7-(aminomethyl)-7-deazaguanine-tRNATyr Escherichia coli
sinefungin competitive inhibition against S-adenosyl-L-methionine and noncompetitive inhibition against 7-(aminomethyl)-7-deazaguanine-tRNATyr Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0019
-
 7-(aminomethyl)-7-deazaguanine-tRNATyr in 100 mM glycylglycine (pH 8.7), 100 mM KCl, 0.5 mM dithiothreitol, 100 mM EDTA, at 37°C Escherichia coli
0.098
-
 S-adenosyl-L-methionine in 100 mM glycylglycine (pH 8.7), 100 mM KCl, 0.5 mM dithiothreitol, 100 mM EDTA, at 37°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + 7-(aminomethyl)-7-deazaguanine-tRNATyr
-
 Escherichia coli L-methionine + adenine + epoxyqueuosine-tRNATyr
-
 ?

Synonyms

Synonyms Comment Organism
QueA
-
 Escherichia coli
S-adenosylmethionine:tRNA ribosyltransferase-isomerase
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0008
-
 S-adenosylhomocysteine competitive inhibition against 7-(aminomethyl)-7-deazaguanine-tRNATyr, in 100 mM glycylglycine (pH 8.7), 100 mM KCl, 0.5 mM dithiothreitol, 100 mM EDTA, at 37°C Escherichia coli
0.0046
-
 sinefungin competitive inhibition against S-adenosyl-L-methionine, in 100 mM glycylglycine (pH 8.7), 100 mM KCl, 0.5 mM dithiothreitol, 100 mM EDTA, at 37°C Escherichia coli
0.0055
-
 sinefungin competitive inhibition against 7-(aminomethyl)-7-deazaguanine-tRNATyr, in 100 mM glycylglycine (pH 8.7), 100 mM KCl, 0.5 mM dithiothreitol, 100 mM EDTA, at 37°C Escherichia coli
0.133
-
 S-adenosylhomocysteine competitive inhibition against S-adenosyl-L-methionine, in 100 mM glycylglycine (pH 8.7), 100 mM KCl, 0.5 mM dithiothreitol, 100 mM EDTA, at 37°C Escherichia coli