Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | weak activation | Saccharolobus shibatae | |
GTP | strong activation at pH 6 and at pH 8. The concentration of GTP required for half-maximal activation is about 0.080 mM | Saccharolobus shibatae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CTP | - |
Saccharolobus shibatae | |
UMP | - |
Saccharolobus shibatae | |
UTP | - |
Saccharolobus shibatae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
Uracil | pH 6.1, without GTP | Saccharolobus shibatae | |
0.002 | - |
Uracil | pH 8.0, without GTP | Saccharolobus shibatae | |
0.004 | - |
Uracil | pH 6.1, presence of 1 mM GTP | Saccharolobus shibatae | |
0.005 | - |
Uracil | pH 8.0, presence of 1 mM GTP | Saccharolobus shibatae | |
0.016 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 8.0, without GTP | Saccharolobus shibatae | |
0.049 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 8.0, presence of 1 mM GTP | Saccharolobus shibatae | |
0.079 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 6.1, without GTP | Saccharolobus shibatae | |
0.115 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 6.1, presence of 1 mM GTP | Saccharolobus shibatae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | UPRTase can use Mn2+, Co2+ and Zn2+ instead of Mg2+. While the saturation curve for Mg2+ is smoothly increasing, these three metal ions are inhibitory at higher concentrations | Saccharolobus shibatae | |
Mg2+ | absolute requirement for Mg2+ | Saccharolobus shibatae | |
Mn2+ | UPRTase can use Mn2+, Co2+ and Zn2+ instead of Mg2+. While the saturation curve for Mg2+ is smoothly increasing, these three metal ions are inhibitory at higher concentrations | Saccharolobus shibatae | |
Zn2+ | UPRTase can use Mn2+, Co2+ and Zn2+ instead of Mg2+. While the saturation curve for Mg2+ is smoothly increasing, these three metal ions are inhibitory at higher concentrations | Saccharolobus shibatae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
200000 | 250000 | gel filtration | Saccharolobus shibatae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus shibatae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Saccharolobus shibatae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.138 | - |
- |
Saccharolobus shibatae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Saccharolobus shibatae | UMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UPRTase | - |
Saccharolobus shibatae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
optimal temperature exceeds 95°C | Saccharolobus shibatae |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
stable for at least 1 h | Saccharolobus shibatae |
90 | - |
half-life: 4 min | Saccharolobus shibatae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
- |
Saccharolobus shibatae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 7 | pH 5.8: about 55% of maximal activity, pH 7.0: about 45% of maximal activity | Saccharolobus shibatae |