Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GTP | strong stimulation, binds cooperatively with 5-phosphoribosyl 1-diphosphate | Saccharolobus solfataricus | |
additional information | the enzyme undergoes a transition from a weakly active or inactive T-state, favored by binding of UMP and CTP, to an active R-state, favored by binding of GTP and 5-phosphoribosyl 1-diphosphate | Saccharolobus solfataricus |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CTP | powerful inhibitor in combination with UMP. CTP and UMP can be bound simultaneously | Saccharolobus solfataricus | |
additional information | the enzyme undergoes a transition from a weakly active or inactive T-state, favored by binding of UMP and CTP, to an active R-state, favored by binding of GTP and 5-phosphoribosyl 1-diphosphate | Saccharolobus solfataricus | |
UMP | powerful inhibitor in combination with CTP. CTP and UMP can be bound simultaneously | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
98000 | - |
sucrose density gradient centrifugation | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Saccharolobus solfataricus | UMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
UPRTase | - |
Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
5-phospho-alpha-D-ribose 1-diphosphate | unactivated enzyme | Saccharolobus solfataricus | |
0.36 | - |
Uracil | unactivated enzyme | Saccharolobus solfataricus | |
6.1 | - |
5-phospho-alpha-D-ribose 1-diphosphate | activated by 1 mM GTP | Saccharolobus solfataricus | |
6.1 | - |
Uracil | activated by 1 mM GTP | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
at 60°C | Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8 | pH 3.8: no activity, pH 5.5: optimum, pH 8: about 20% of maximal activity | Saccharolobus solfataricus |