Literature summary for 2.4.2.9 extracted from

Barchue, J.; Symersky, J.; Narayana, S.V.; Moore, J.K.; DeLucas, L.J.; Chattopadhyay, D.
Expression, purification, crystallization and preliminary X-ray diffraction analysis of uracil phosphoribosyltransferase of Toxoplasma gondii (1999), Acta Crystallogr. Sect. D, 55, 347-349.

No PubMed abstract available

Search for more literature for 2.4.2.9

Cloned(Commentary)

Cloned (Comment)	Organism
DNA sequence determination, functional overexpression in Escherichia coli BL21(DE3)plyS	Toxoplasma gondii

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop-vapour diffusion technique, 2.0 M ammonium phosphate as precipitant, pH 8.0, room temperature, X-ray diffraction structure analysis, crystals are enzymatically active, wild-type and mutant enzymes	Toxoplasma gondii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28000	-	x * 28000, recombinant protein, SDS-PAGE	Toxoplasma gondii

Organism

Organism	UniProt	Comment	Textmining
Toxoplasma gondii	Q26998	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant from Escherichia coli	Toxoplasma gondii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
uracil + 5-phospho-alpha-D-ribose 1-diphosphate	-	Toxoplasma gondii	UMP + diphosphate	-	?

Subunits

Subunits	Comment	Organism
?	x * 28000, recombinant protein, SDS-PAGE	Toxoplasma gondii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	8	-	Toxoplasma gondii

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Release 2023.1 (January 2023)