Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, overexpression in enzyme-deficient Escherichia coli | [Bacillus] caldolyticus |
General Stability | Organism |
---|---|
bovine serum albumin at 2 mg/ml stabilizes | [Bacillus] caldolyticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
Uracil | - |
[Bacillus] caldolyticus | |
0.05 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
[Bacillus] caldolyticus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
gel filtration | [Bacillus] caldolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis 168 | - |
- |
- |
[Bacillus] caldolyticus | P70881 | gene upp | - |
[Bacillus] caldolyticus | P70881 | strain DSM 405 | - |
Purification (Comment) | Organism |
---|---|
recombinant from enzyme-deficient Escherichia coli | [Bacillus] caldolyticus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.07 | - |
37°C | Bacillus subtilis |
0.09 | - |
60°C | [Bacillus] caldolyticus |
19.2 | - |
purified recombinant enzyme, 37°C | [Bacillus] caldolyticus |
Storage Stability | Organism |
---|---|
0°C, enzyme concentration 0.001 mg/ml, 50% loss of activity in 1 h | [Bacillus] caldolyticus |
4°C, concentrated enzyme is stable for several months, on dilution occurs rapid loss of activity | [Bacillus] caldolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Bacillus subtilis | UMP + diphosphate | - |
? | |
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
[Bacillus] caldolyticus | UMP + diphosphate | - |
? | |
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Bacillus subtilis 168 | UMP + diphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
recombinant enzyme | [Bacillus] caldolyticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
10 min, 90% remaining activity | Bacillus subtilis |
55 | - |
10 min, 20% remaining activity | Bacillus subtilis |
60 | - |
10 min, inactivation | Bacillus subtilis |
70 | - |
10 min, 70% remaining activity | [Bacillus] caldolyticus |
75 | - |
10 min, 5% remaining activity | [Bacillus] caldolyticus |
80 | - |
10 min, inactivation | [Bacillus] caldolyticus |