Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GTP | kinetics | Escherichia coli | |
GTP | highly activating at low 5-phosphoribose 1-diphosphate concentration, e.g. 0.2 mM, by modification of the structure from dimer/trimer to pentamer/hexamer | Escherichia coli | |
GTP | about 5-7fold increase of Km for 5-phosphoribose 1-diphosphate, unaltered Vmax | Escherichia coli | |
guanosine-3',5'-diphosphate | activates in the same range as GTP | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
overexpressing from plasmid in Escherichia coli strain NF1815 | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-phosphoribose 1-diphosphate | inhibitory when higher concentrated as Mg2+ | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | poor activator | Escherichia coli | |
Mg2+ | required | Escherichia coli | |
Mn2+ | can partly substitute for Mg2+ at 5 mM, less effective, inhibitory at higher concentration | Escherichia coli | |
Zn2+ | can partly substitute for Mg2+ at 5 mM, less effective, inhibitory at higher concentration | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
gel filtration and sedimentation analyses: a smaller oligomeric, less active form, dimeric or trimeric, predominates in absence of substrates, a larger aggregated, more active form, pentameric or hexameric, predominates in presence of substrates | Escherichia coli |
22500 | - |
x * 22500, SDS-PAGE | Escherichia coli |
63000 | - |
sucrose density gradient centrifugation and gel filtration in absence of substrates | Escherichia coli |
111000 | - |
sucrose density gradient centrifugation and gel filtration in presence of substrates | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene upp | - |
Purification (Comment) | Organism |
---|---|
recombinant from overexpressing strain | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.5 | - |
purified recombinant enzyme | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Escherichia coli | 5-fluoro-UMP + diphosphate | - |
? | |
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Escherichia coli | UMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 22500, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Escherichia coli |