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Literature summary for 2.4.2.9 extracted from
- Different oligomeric states are involved in the allosteric behavior of uracil phosphoribosyltransferase from Escherichia coli (1996), Eur. J. Biochem., 240, 637-645.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| GTP | kinetics | Escherichia coli |  |
| GTP | highly activating at low 5-phosphoribose 1-diphosphate concentration, e.g. 0.2 mM, by modification of the structure from dimer/trimer to pentamer/hexamer | Escherichia coli | |
| GTP | about 5-7fold increase of Km for 5-phosphoribose 1-diphosphate, unaltered Vmax | Escherichia coli | |
| guanosine-3',5'-diphosphate | activates in the same range as GTP | Escherichia coli | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpressing from plasmid in Escherichia coli strain NF1815 | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5-phosphoribose 1-diphosphate | inhibitory when higher concentrated as Mg2+ | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Escherichia coli |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | poor activator | Escherichia coli | |
| Mg2+ | required | Escherichia coli | |
| Mn2+ | can partly substitute for Mg2+ at 5 mM, less effective, inhibitory at higher concentration | Escherichia coli | |
| Zn2+ | can partly substitute for Mg2+ at 5 mM, less effective, inhibitory at higher concentration | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
gel filtration and sedimentation analyses: a smaller oligomeric, less active form, dimeric or trimeric, predominates in absence of substrates, a larger aggregated, more active form, pentameric or hexameric, predominates in presence of substrates | Escherichia coli |
| 22500 | - |
x * 22500, SDS-PAGE | Escherichia coli |
| 63000 | - |
sucrose density gradient centrifugation and gel filtration in absence of substrates | Escherichia coli |
| 111000 | - |
sucrose density gradient centrifugation and gel filtration in presence of substrates | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
gene upp | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant from overexpressing strain | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
purified recombinant enzyme | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Escherichia coli | 5-fluoro-UMP + diphosphate | - |
? | |
| uracil + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Escherichia coli | UMP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 22500, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Escherichia coli |
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