Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GTP | no activation | Toxoplasma gondii |
Application | Comment | Organism |
---|---|---|
medicine | enzyme is a possible therapeutic target | Toxoplasma gondii |
Cloned (Comment) | Organism |
---|---|
functional overexpression in Escherichia coli JM109 | Toxoplasma gondii |
General Stability | Organism |
---|---|
rapid precipitation of purified enzyme without thiol reducing agents | Toxoplasma gondii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluorouracil | - |
Toxoplasma gondii | |
GTP | slight inhibition | Toxoplasma gondii | |
additional information | no inhibition by thymine, cytosine, orotic acid, and hypoxanthine | Toxoplasma gondii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
Uracil | recombinant enzyme | Toxoplasma gondii | |
0.243 | - |
5-phospho-alpha-D-ribose 1-diphosphate | recombinant enzyme | Toxoplasma gondii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | no activation | Toxoplasma gondii | |
Co2+ | can substitute Mg2+ | Toxoplasma gondii | |
divalent cations | absolute requirement | Toxoplasma gondii | |
Mg2+ | required | Toxoplasma gondii | |
Mn2+ | can substitute for Mg2+ | Toxoplasma gondii | |
additional information | no activation by Ba2+ | Toxoplasma gondii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | 27600 | recombinant enzyme, gel filtration and DNA sequence determination | Toxoplasma gondii |
27000 | - |
1 * 27000, recombinant enzyme, SDS-PAGE | Toxoplasma gondii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | Toxoplasma gondii | pyrimidine salvage enzyme, no de novo synthesis of pyrmidines | UMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Toxoplasma gondii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Toxoplasma gondii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
1051 DELTAA252/min/mg | Toxoplasma gondii |
Storage Stability | Organism |
---|---|
4°C, purified enzyme, TMD50 buffer, stable for at least 1 week | Toxoplasma gondii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate | no activity | Toxoplasma gondii | 5-fluoro-UMP + diphosphate | - |
? | |
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | highly specific for uracil | Toxoplasma gondii | UMP + diphosphate | - |
? | |
uracil + 5-phospho-alpha-D-ribose 1-diphosphate | pyrimidine salvage enzyme, no de novo synthesis of pyrmidines | Toxoplasma gondii | UMP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 27000, recombinant enzyme, SDS-PAGE | Toxoplasma gondii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Toxoplasma gondii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
begins to unfold irreversibly | Toxoplasma gondii |
65 | - |
complete inactivation after 5 min | Toxoplasma gondii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
5-fluorouracil | - |
Toxoplasma gondii |