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Literature summary for 2.4.2.9 extracted from

  • Carter, D.; Donald, R.G.K.; Roos, D.; Ullman, B.
    Expression, purification, and characterization of uracil phosphoribosyltransferase from Toxoplasma gondii (1997), Mol. Biochem. Parasitol., 87, 137-144.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
GTP no activation Toxoplasma gondii

Application

Application Comment Organism
medicine enzyme is a possible therapeutic target Toxoplasma gondii

Cloned(Commentary)

Cloned (Comment) Organism
functional overexpression in Escherichia coli JM109 Toxoplasma gondii

General Stability

General Stability Organism
rapid precipitation of purified enzyme without thiol reducing agents Toxoplasma gondii

Inhibitors

Inhibitors Comment Organism Structure
5-fluorouracil
-
Toxoplasma gondii
GTP slight inhibition Toxoplasma gondii
additional information no inhibition by thymine, cytosine, orotic acid, and hypoxanthine Toxoplasma gondii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0035
-
Uracil recombinant enzyme Toxoplasma gondii
0.243
-
5-phospho-alpha-D-ribose 1-diphosphate recombinant enzyme Toxoplasma gondii

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ no activation Toxoplasma gondii
Co2+ can substitute Mg2+ Toxoplasma gondii
divalent cations absolute requirement Toxoplasma gondii
Mg2+ required Toxoplasma gondii
Mn2+ can substitute for Mg2+ Toxoplasma gondii
additional information no activation by Ba2+ Toxoplasma gondii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000 27600 recombinant enzyme, gel filtration and DNA sequence determination Toxoplasma gondii
27000
-
1 * 27000, recombinant enzyme, SDS-PAGE Toxoplasma gondii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
uracil + 5-phospho-alpha-D-ribose 1-diphosphate Toxoplasma gondii pyrimidine salvage enzyme, no de novo synthesis of pyrmidines UMP + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli Toxoplasma gondii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
1051 DELTAA252/min/mg Toxoplasma gondii

Storage Stability

Storage Stability Organism
4°C, purified enzyme, TMD50 buffer, stable for at least 1 week Toxoplasma gondii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-fluorouracil + 5-phospho-alpha-D-ribose 1-diphosphate no activity Toxoplasma gondii 5-fluoro-UMP + diphosphate
-
?
uracil + 5-phospho-alpha-D-ribose 1-diphosphate highly specific for uracil Toxoplasma gondii UMP + diphosphate
-
?
uracil + 5-phospho-alpha-D-ribose 1-diphosphate pyrimidine salvage enzyme, no de novo synthesis of pyrmidines Toxoplasma gondii UMP + diphosphate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 27000, recombinant enzyme, SDS-PAGE Toxoplasma gondii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Toxoplasma gondii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
begins to unfold irreversibly Toxoplasma gondii
65
-
complete inactivation after 5 min Toxoplasma gondii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.025
-
5-fluorouracil
-
Toxoplasma gondii