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Literature summary for 2.4.2.8 extracted from
- Hypoxanthine-guanine phosphoribosyltransferase/adenylate kinase from Zobellia galactanivorans a bifunctional catalyst for the synthesis of nucleoside-5-mono-, di- and triphosphates (2020), Front. Bioeng. Biotechnol., 8, 677 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | potential of ZgHGPRT/AMPK as biocatalyst for the synthesis of nucleoside-50-mono-, di-, and triphosphates | Zobellia galactanivorans |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| sequence comparisons, recombinant expression of N-terminally His6-tagged enzyme with a thrombin cleavage site in Escherichia coli strain BL21(DE3) | Zobellia galactanivorans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Zobellia galactanivorans |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Zobellia galactanivorans | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | preferred metal ion | Zobellia galactanivorans |  |
| Mg2+ | preferred metal ion | Zobellia galactanivorans | |
| additional information | the enzyme is dependent on divalent cations | Zobellia galactanivorans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanosine + 5-phospho-alpha-D-ribose 1-diphosphate | Zobellia galactanivorans | - |
GMP + diphosphate | - |
? | |
| guanosine + 5-phospho-alpha-D-ribose 1-diphosphate | Zobellia galactanivorans CCUG 47099 | - |
GMP + diphosphate | - |
? | |
| guanosine + 5-phospho-alpha-D-ribose 1-diphosphate | Zobellia galactanivorans Dsij | - |
GMP + diphosphate | - |
? | |
| guanosine + 5-phospho-alpha-D-ribose 1-diphosphate | Zobellia galactanivorans DSM 12802 | - |
GMP + diphosphate | - |
? | |
| guanosine + 5-phospho-alpha-D-ribose 1-diphosphate | Zobellia galactanivorans CIP 106680 | - |
GMP + diphosphate | - |
? | |
| guanosine + 5-phospho-alpha-D-ribose 1-diphosphate | Zobellia galactanivorans NCIMB 13871 | - |
GMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Zobellia galactanivorans | - |
IMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Zobellia galactanivorans CCUG 47099 | - |
IMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Zobellia galactanivorans Dsij | - |
IMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Zobellia galactanivorans DSM 12802 | - |
IMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Zobellia galactanivorans CIP 106680 | - |
IMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Zobellia galactanivorans NCIMB 13871 | - |
IMP + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zobellia galactanivorans | G0LC40 | - |
- |
| Zobellia galactanivorans CCUG 47099 | G0LC40 | - |
- |
| Zobellia galactanivorans CIP 106680 | G0LC40 | - |
- |
| Zobellia galactanivorans Dsij | G0LC40 | - |
- |
| Zobellia galactanivorans DSM 12802 | G0LC40 | - |
- |
| Zobellia galactanivorans NCIMB 13871 | G0LC40 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage through thrombin, and gel filtration | Zobellia galactanivorans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.47 | - |
substrate guanosine, pH 8.0, 50°C, with 2.4 mM Mg2+ | Zobellia galactanivorans |
| 0.53 | - |
substrate hypoxanthine, pH 7.0, 50°C, with 2.4 mM Mg2+ | Zobellia galactanivorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanosine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Zobellia galactanivorans | GMP + diphosphate | - |
? | |
| guanosine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Zobellia galactanivorans CCUG 47099 | GMP + diphosphate | - |
? | |
| guanosine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Zobellia galactanivorans Dsij | GMP + diphosphate | - |
? | |
| guanosine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Zobellia galactanivorans DSM 12802 | GMP + diphosphate | - |
? | |
| guanosine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Zobellia galactanivorans CIP 106680 | GMP + diphosphate | - |
? | |
| guanosine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Zobellia galactanivorans NCIMB 13871 | GMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Zobellia galactanivorans | IMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Zobellia galactanivorans CCUG 47099 | IMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Zobellia galactanivorans Dsij | IMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Zobellia galactanivorans DSM 12802 | IMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Zobellia galactanivorans CIP 106680 | IMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Zobellia galactanivorans NCIMB 13871 | IMP + diphosphate | - |
? | |
| additional information | ZgHGPRT/AMPK is a bifunctional enzyme, which acts as phosphoribosyltransferase or adenylate kinase depending upon the nature of the substrate. Substrate specificity, overview | Zobellia galactanivorans | ? | - |
- |
|
| additional information | ZgHGPRT/AMPK is a bifunctional enzyme, which acts as phosphoribosyltransferase or adenylate kinase depending upon the nature of the substrate. Substrate specificity, overview | Zobellia galactanivorans CCUG 47099 | ? | - |
- |
|
| additional information | ZgHGPRT/AMPK is a bifunctional enzyme, which acts as phosphoribosyltransferase or adenylate kinase depending upon the nature of the substrate. Substrate specificity, overview | Zobellia galactanivorans Dsij | ? | - |
- |
|
| additional information | ZgHGPRT/AMPK is a bifunctional enzyme, which acts as phosphoribosyltransferase or adenylate kinase depending upon the nature of the substrate. Substrate specificity, overview | Zobellia galactanivorans DSM 12802 | ? | - |
- |
|
| additional information | ZgHGPRT/AMPK is a bifunctional enzyme, which acts as phosphoribosyltransferase or adenylate kinase depending upon the nature of the substrate. Substrate specificity, overview | Zobellia galactanivorans CIP 106680 | ? | - |
- |
|
| additional information | ZgHGPRT/AMPK is a bifunctional enzyme, which acts as phosphoribosyltransferase or adenylate kinase depending upon the nature of the substrate. Substrate specificity, overview | Zobellia galactanivorans NCIMB 13871 | ? | - |
- |
|
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 41650, sedimentation velocity and SDS-PAGE, 2 * 43770, sequence caluclation | Zobellia galactanivorans |
| More | quaternary structure analysis | Zobellia galactanivorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hypoxanthine-guanine phosphoribosyltransferase/adenylate kinase | - |
Zobellia galactanivorans |
| More | cf. EC 2.7.4.3 | Zobellia galactanivorans |
| ZgHGPRT/AMPK | - |
Zobellia galactanivorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 60 | - |
Zobellia galactanivorans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 80 | ZgHGPRT/AMPK displays over 70% of maximal activity across the broad temperature range of 30-80°C, with a maximum at 50-60°C | Zobellia galactanivorans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 45 | purified recombinant ZgHGPRT/AMPK exhibits an apparent unfolding temperature (Tm) of 45°C and a retained activity of around 80% when incubated at 40°C for 240 min | Zobellia galactanivorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Zobellia galactanivorans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | ZgHGPRT/AMPK displays high activity in a narrow pH range 6.0-7.0, with a maximum peak in 50 mM sodium phosphate at pH 7.0 | Zobellia galactanivorans |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | adenylate kinase (EC 2.7.4.3, AMPK) belongs to nucleoside-5'-monophosphate kinase (NMPK) family. ZgHGPRT/AMPK belongs to class I PRTs, which display a conserved 13-residue fingerprint region (PRPP binding-motif) in their amino acid sequence | Zobellia galactanivorans |
| additional information | identification of a putative dual-domain hypoxanthine-guanine phosphoribosyltransferase (HGPRT)/adenylate kinase (AMPK). ZgHGPRT/AMPK single domain, dual domain 3D structure homology modeling, HGPRT from Leptospira interrogans (PDB ID 4QRI) and AMPK structure from Geobacillus stearothermophilus (PDB ID 1ZIN) are used as templates. ZgHGPRT/AMPK model is complexed with Hyp, PRPP and Mg2+ in the active site of the HGPRT domain, and with AMP, ATP and Mg2+ in the active site of AMPK domain. Essential elements of type I PRTs architecture are found in ZgHGPRT/AMPK amino acid sequence such as diphosphate loop, the flexible loop and PRPP binding domain (including PRPP loop), as well as in the homology model | Zobellia galactanivorans |
| physiological function | the Zobellia galactanivorans enzyme contains both HGPRT (N-terminal part) and AMPK (C-terminal part) domains. The N-terminal HGPRT module is involved in the purine salvage and converts hypoxanthine to inosine-5'-monophosphate (IMP) and guanine to guanosine-5'-monophosphate (GMP) | Zobellia galactanivorans |
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