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Literature summary for 2.4.2.8 extracted from
- Evaluating iso-mukaadial acetate and ursolic acid acetate as Plasmodium falciparum hypoxanthine-guanine-xanthine phosphoribosyltransferase inhibitors (2019), Biomolecules, 9, 861 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene PF3D7_1012400, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Plasmodium falciparum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iso-mukaadial acetate | - |
Plasmodium falciparum |  |
| additional information | spectrometric enzyme-inhibitor binding analysis, molecular docking and molecular dynamics studies, computational binding affinity analysis, binding mode and kinetics, overview. Iso-mukaadial acetate and ursolic acid acetate ligands are docked into the binding sites of Plasmodium falciparum and human hypoxanthine-guanine phosphoribosyl transferases protein using the glide docking module, binding structure comparisons | Plasmodium falciparum | |
| triethyl-2-phosphonobutyrate | - |
Plasmodium falciparum | |
| ursolic acid acetate | - |
Plasmodium falciparum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Plasmodium falciparum | - |
IMP + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | Q8IJS1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity xhromatography | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Plasmodium falciparum | IMP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | structure comparison with the human enzyme | Plasmodium falciparum |
| More | the enzyme occurs as a mixture of monomer and dimer, and undergoes tetramerisation on the addition of phosphoribosyl diphosphate | Plasmodium falciparum |
| tetramer | 4 * 28000, recombinant His-tagged enzyme, SDS-PAGE | Plasmodium falciparum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HGXPRT | - |
Plasmodium falciparum |
| hypoxanthine-guanine-xanthine phosphoribosyltransferase | - |
Plasmodium falciparum |
| More | cf. EC 2.4.2.22 | Plasmodium falciparum |
| PF3D7_1012400 | - |
Plasmodium falciparum |
| PfHGXPRT | - |
Plasmodium falciparum |
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