Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.2.8 extracted from

  • Roy, S.; Nagappa, L.K.; Prahladarao, V.S.; Balaram, H.
    Kinetic mechanism of Plasmodium falciparum hypoxanthine-guanine-xanthine phosphoribosyltransferase (2015), Mol. Biochem. Parasitol., 204, 111-120.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
IMP
-
Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
9-deazaguanine uncompetitive inhibitor Plasmodium falciparum
GMP competitive inhibitor with respect to guanine, xanthine, and 5-phospho-alpha-D-ribose 1-diphosphate Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0016
-
IMP unactivated enzyme, at pH 7.4 and 23°C Plasmodium falciparum
0.0039
-
diphosphate unactivated enzyme, at pH 7.4 and 23°C Plasmodium falciparum
0.0042
-
diphosphate IMP-activated enzyme, at pH 7.4 and 23°C Plasmodium falciparum
0.007
-
IMP IMP-activated enzyme, at pH 7.4 and 23°C Plasmodium falciparum
0.053
-
5-phospho-alpha-D-ribose 1-diphosphate IMP-activated enzyme, at pH 7.4 and 23°C Plasmodium falciparum
0.078
-
xanthine unactivated enzyme, at pH 7.4 and 23°C Plasmodium falciparum
0.101
-
xanthine IMP-activated enzyme, at pH 7.4 and 23°C Plasmodium falciparum
1.08
-
5-phospho-alpha-D-ribose 1-diphosphate unactivated enzyme, at pH 7.4 and 23°C Plasmodium falciparum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GMP + diphosphate Plasmodium falciparum
-
guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
IMP + diphosphate Plasmodium falciparum
-
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
XMP + diphosphate Plasmodium falciparum
-
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum P20035
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GMP + diphosphate
-
Plasmodium falciparum guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
IMP + diphosphate
-
Plasmodium falciparum hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
r
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Plasmodium falciparum XMP + diphosphate
-
r
XMP + diphosphate
-
Plasmodium falciparum xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
r

Synonyms

Synonyms Comment Organism
HGXPRT
-
Plasmodium falciparum
hypoxanthine-guanine-xanthine phosphoribosyltransferase
-
Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0036
-
IMP unactivated enzyme, at pH 7.4 and 23°C Plasmodium falciparum
0.0042
-
IMP IMP-activated enzyme, at pH 7.4 and 23°C Plasmodium falciparum
0.2
-
5-phospho-alpha-D-ribose 1-diphosphate unactivated enzyme, at pH 7.4 and 23°C Plasmodium falciparum
0.2
-
xanthine unactivated enzyme, at pH 7.4 and 23°C Plasmodium falciparum
1.6
-
5-phospho-alpha-D-ribose 1-diphosphate IMP-activated enzyme, at pH 7.4 and 23°C Plasmodium falciparum
1.6
-
xanthine IMP-activated enzyme, at pH 7.4 and 23°C Plasmodium falciparum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.18
-
5-phospho-alpha-D-ribose 1-diphosphate unactivated enzyme, at pH 7.4 and 23°C Plasmodium falciparum
0.6
-
IMP IMP-activated enzyme, at pH 7.4 and 23°C Plasmodium falciparum
0.9
-
diphosphate unactivated enzyme, at pH 7.4 and 23°C Plasmodium falciparum
1
-
diphosphate IMP-activated enzyme, at pH 7.4 and 23°C Plasmodium falciparum
2.2
-
IMP unactivated enzyme, at pH 7.4 and 23°C Plasmodium falciparum
2.6
-
xanthine unactivated enzyme, at pH 7.4 and 23°C Plasmodium falciparum
16
-
xanthine IMP-activated enzyme, at pH 7.4 and 23°C Plasmodium falciparum
30
-
5-phospho-alpha-D-ribose 1-diphosphate IMP-activated enzyme, at pH 7.4 and 23°C Plasmodium falciparum