Cloned (Comment) | Organism |
---|---|
HGPRT, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21 (DE3) | Mycobacterium tuberculosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, recombinant enzyme, overview | Mycobacterium tuberculosis | |
0.025 | - |
guanine | pH 7.4, 25°C, recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | |
0.05 | - |
hypoxanthine | pH 7.4, 25°C, recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | |
0.36 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 25°C, recombinant enzyme, with guanine | Mycobacterium tuberculosis | |
2.039 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 25°C, recombinant enzyme, with hypoxanthine | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | - |
GMP + diphosphate | - |
? | |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis H37Rv | - |
GMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | - |
IMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis H37Rv | - |
IMP + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain BL21 (DE3) 1-2fold to homogeneity by anion exchange chromatography, gel filtration, and hydrophobic interaction chromatography | Mycobacterium tuberculosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.4 | - |
purified recombinant enzyme, substrate guanine | Mycobacterium tuberculosis |
1.44 | - |
purified recombinant enzyme, substrate hypoxanthine | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis | GMP + diphosphate | - |
? | |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis H37Rv | GMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis | IMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis H37Rv | IMP + diphosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HGPRT | - |
Mycobacterium tuberculosis |
hypoxanthine-guanine phosphoribosyltransferase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0139 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 25°C, recombinant enzyme, with guanine | Mycobacterium tuberculosis | |
0.14 | - |
guanine | pH 7.4, 25°C, recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | |
0.9 | - |
hypoxanthine | pH 7.4, 25°C, recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | |
0.97 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 25°C, recombinant enzyme, with hypoxanthine | Mycobacterium tuberculosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mycobacterium tuberculosis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.6 | - |
guanine | pH 7.4, 25°C, recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | |
18 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 25°C, recombinant enzyme, with guanine | Mycobacterium tuberculosis | |
386 | - |
hypoxanthine | pH 7.4, 25°C, recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | |
476 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 25°C, recombinant enzyme, with hypoxanthine | Mycobacterium tuberculosis |