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Literature summary for 2.4.2.8 extracted from
- Hypoxanthine-guanine phosphoribosyltransferase from Mycobacterium tuberculosis H37Rv: cloning, expression, and biochemical characterization (2009), Protein Expr. Purif., 66, 185-190.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| HGPRT, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21 (DE3) | Mycobacterium tuberculosis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | steady-state kinetics, recombinant enzyme, overview | Mycobacterium tuberculosis | |
| 0.025 | - |
guanine | pH 7.4, 25°C, recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis |  |
| 0.05 | - |
hypoxanthine | pH 7.4, 25°C, recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | |
| 0.36 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 25°C, recombinant enzyme, with guanine | Mycobacterium tuberculosis | |
| 2.039 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 25°C, recombinant enzyme, with hypoxanthine | Mycobacterium tuberculosis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | - |
GMP + diphosphate | - |
? | |
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis H37Rv | - |
GMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | - |
IMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis H37Rv | - |
IMP + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21 (DE3) 1-2fold to homogeneity by anion exchange chromatography, gel filtration, and hydrophobic interaction chromatography | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.4 | - |
purified recombinant enzyme, substrate guanine | Mycobacterium tuberculosis |
| 1.44 | - |
purified recombinant enzyme, substrate hypoxanthine | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis | GMP + diphosphate | - |
? | |
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis H37Rv | GMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis | IMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mycobacterium tuberculosis H37Rv | IMP + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HGPRT | - |
Mycobacterium tuberculosis |
| hypoxanthine-guanine phosphoribosyltransferase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0139 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 25°C, recombinant enzyme, with guanine | Mycobacterium tuberculosis | |
| 0.14 | - |
guanine | pH 7.4, 25°C, recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | |
| 0.9 | - |
hypoxanthine | pH 7.4, 25°C, recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | |
| 0.97 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 25°C, recombinant enzyme, with hypoxanthine | Mycobacterium tuberculosis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Mycobacterium tuberculosis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.6 | - |
guanine | pH 7.4, 25°C, recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | |
| 18 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 25°C, recombinant enzyme, with guanine | Mycobacterium tuberculosis | |
| 386 | - |
hypoxanthine | pH 7.4, 25°C, recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate | Mycobacterium tuberculosis | |
| 476 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.4, 25°C, recombinant enzyme, with hypoxanthine | Mycobacterium tuberculosis | |
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