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Literature summary for 2.4.2.8 extracted from
- Application of HPLC to study the kinetics of a branched bi-enzyme system consisting of hypoxanthine-guanine phosphoribosyltransferase and xanthine oxidase - an important biochemical system to evaluate the efficiency of the anticancer drug 6-mercaptopurine (2007), J. Chromatogr. B, 850, 7-14.
Application
| Application | Comment | Organism |
|---|---|---|
| analysis | the branched bi-enzyme system with xanthine oxidase is an important biochemical system to evaluate the efficiency of the anticancer drug 6-mercaptopurine, overview | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli strain BL21(DE3) | Homo sapiens |
| gene HGPRT, expression in Escherichia coli strain BL21(DE3) | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic analysis of the branched bi-enzyme system, detailed overview | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Homo sapiens | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Homo sapiens | - |
IMP + diphosphate | - |
? | |
| additional information | Homo sapiens | the enzyme is organized in a branched bi-enzyme system with xanthine oxidase, EC 1.17.3.2 | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| acute lymphoblastic leukemia cell | - |
Homo sapiens | - |
| JURKAT cell | - |
Homo sapiens | - |
| JURKAT cell | a T-ALL cell line | Homo sapiens | - |
| lymphocyte | immature | Homo sapiens | - |
| T-ALL cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-mercaptopurine + 5-phospho-alpha-D-ribose 1-diphosphate | a thiopurine antimetabolite | Homo sapiens | thioinosinic monophosphate + diphosphate | a therapeutically active metabolite | ? | |
| 6-mercaptopurine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | 6-thioinosine monophosphate + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Homo sapiens | IMP + diphosphate | - |
? | |
| additional information | the enzyme is organized in a branched bi-enzyme system with xanthine oxidase, EC 1.17.3.2 | Homo sapiens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| HGPRT | - |
Homo sapiens |
| hypoxanthine-guanine phosphoribosyltransferase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Homo sapiens |
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Release 2023.1 (January 2023)
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