Application | Comment | Organism |
---|---|---|
pharmacology | the enzyme is a target for mechanism-based design of specific inhibitors | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-phospho-alpha-D-ribose 1-diphosphate | competitive versus diphosphate and GMP or IMP | Trypanosoma cruzi | |
diphosphate | competitive versus 5-phospho-alpha-D-ribose 1-diphosphate, uncompetitive versus hypoxanthine or guanine | Trypanosoma cruzi | |
GMP | competitive versus 5-phospho-alpha-D-ribose 1-diphosphate, noncompetitive versus guanine | Trypanosoma cruzi | |
IMP | competitive versus 5-phospho-alpha-D-ribose 1-diphosphate, noncompetitive versus hypoxanthine | Trypanosoma cruzi | |
additional information | product inhibition study, the substrates/products protect the enzyme against digestion by trypsin, especially hypoxanthine with diphosphate | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics and dissociation constants | Trypanosoma cruzi | |
0.0086 | - |
hypoxanthine | pH 7.5, 37°C, recombinant wild-type enzyme | Trypanosoma cruzi | |
0.012 | - |
guanine | pH 7.5, 37°C, recombinant wild-type enzyme | Trypanosoma cruzi | |
0.016 | - |
IMP | pH 7.5, 37°C, recombinant wild-type enzyme | Trypanosoma cruzi | |
0.016 | - |
diphosphate | pH 7.5, 37°C, recombinant wild-type enzyme, with IMP | Trypanosoma cruzi | |
0.022 | - |
diphosphate | pH 7.5, 37°C, recombinant wild-type enzyme, with GMP | Trypanosoma cruzi | |
0.029 | - |
GMP | pH 7.5, 37°C, recombinant wild-type enzyme | Trypanosoma cruzi | |
0.031 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.5, 37°C, recombinant wild-type enzyme, with hypoxanthine | Trypanosoma cruzi | |
0.032 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.5, 37°C, recombinant wild-type enzyme, with guanine | Trypanosoma cruzi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Trypanosoma cruzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GMP + diphosphate | Trypanosoma cruzi | - |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
IMP + diphosphate | Trypanosoma cruzi | - |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | ordered bi bi reaction mechanism, formation of dead-end complexes with purine substrates and diphosphate | Trypanosoma cruzi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GMP + diphosphate | - |
Trypanosoma cruzi | guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
IMP + diphosphate | - |
Trypanosoma cruzi | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
additional information | formation of dead-end complexes with purine substrates and diphosphate | Trypanosoma cruzi | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HPRT | - |
Trypanosoma cruzi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
GMP | pH 7.5, 37°C, recombinant enzyme | Trypanosoma cruzi | |
0.1 | - |
diphosphate | pH 7.5, 37°C, recombinant enzyme, with GMP | Trypanosoma cruzi | |
0.46 | - |
IMP | pH 7.5, 37°C, recombinant enzyme | Trypanosoma cruzi | |
0.47 | - |
diphosphate | pH 7.5, 37°C, recombinant enzyme, with IMP | Trypanosoma cruzi | |
22.9 | - |
hypoxanthine | pH 7.5, 37°C, recombinant enzyme | Trypanosoma cruzi | |
23.2 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.5, 37°C, recombinant enzyme, with hypoxanthine | Trypanosoma cruzi | |
32.2 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.5, 37°C, recombinant enzyme, with guanine | Trypanosoma cruzi | |
32.9 | - |
guanine | pH 7.5, 37°C, recombinant enzyme | Trypanosoma cruzi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Trypanosoma cruzi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
GMP | pH 7.5, 37°C, product inhibition versus 5-phospho-alpha-D-ribose 1-diphosphate, substrate guanine, recombinant enzyme | Trypanosoma cruzi | |
0.037 | - |
IMP | pH 7.5, 37°C, product inhibition versus 5-phospho-alpha-D-ribose 1-diphosphate, substrate hypoxanthine, recombinant enzyme | Trypanosoma cruzi | |
0.037 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.5, 37°C, product inhibition versus IMP, substrate hypoxanthine, recombinant enzyme | Trypanosoma cruzi | |
0.093 | - |
diphosphate | pH 7.5, 37°C, product inhibition versus 5-phospho-alpha-D-ribose 1-diphosphate, substrate guanine, recombinant enzyme | Trypanosoma cruzi | |
0.1 | - |
GMP | pH 7.5, 37°C, product inhibition versus guanine, substrate guanine, recombinant enzyme | Trypanosoma cruzi | |
0.122 | - |
diphosphate | pH 7.5, 37°C, product inhibition versus 5-phospho-alpha-D-ribose 1-diphosphate, substrate hypoxanthine, recombinant enzyme | Trypanosoma cruzi | |
0.122 | - |
diphosphate | pH 7.5, 37°C, product inhibition versus hypoxanthine, substrate hypoxanthine, recombinant enzyme | Trypanosoma cruzi | |
0.138 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.5, 37°C, product inhibition versus diphosphate, substrate hypoxanthine, recombinant enzyme | Trypanosoma cruzi | |
0.138 | - |
IMP | pH 7.5, 37°C, product inhibition versus hypoxanthine, substrate hypoxanthine, recombinant enzyme | Trypanosoma cruzi | |
0.298 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.5, 37°C, product inhibition versus diphosphate, substrate guanine, recombinant enzyme | Trypanosoma cruzi | |
0.302 | - |
diphosphate | pH 7.5, 37°C, product inhibition versus guanine, substrate guanine, recombinant enzyme | Trypanosoma cruzi | |
0.423 | - |
5-phospho-alpha-D-ribose 1-diphosphate | pH 7.5, 37°C, product inhibition versus GMP, substrate guanine, recombinant enzyme | Trypanosoma cruzi |