Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant L160I enzymes in Escherichia coli strain BL21(DE3) | Caldanaerobacter subterraneus subsp. tengcongensis |
Crystallization (Comment) | Organism |
---|---|
purified recombinant mutant L160I enzyme, hanging drop vapour diffusion method, 0.001 ml of 10 mg/ml protein in 20 mM Tris-HCl, pH 7.4, excess GMP in a molar ratio 4:1, is mixed with equal volume of crystallization solution containing 0.2 M CaCl2 dihydrate, 0.1 M HEPES, pH 7.5, 28% PEG 400, 20°C, 2 weeks, X-ray diffraction structure determination and analysis at 1.7 A resolution, purified recombinant wild-type enzyme, hanging drop vapour diffusion method, 0.001 ml of 10 mg/ml protein in 20 mM Tris-HCl, pH 7.4, excess IMP in a molar ratio 4:1, is mixed with equal volume of crystallization solution containing 0.2 M magnesium acetate tetrahydrate, 0.1 M sodium cacodylate, pH 6.5, 12% PEG 4000, 20°C, within 1 week, X-ray diffraction structure determination and analysis at 2.2 A resolution | Caldanaerobacter subterraneus subsp. tengcongensis |
Protein Variants | Comment | Organism |
---|---|---|
L160I | site-directed mutagenesis, crystal structure determination | Caldanaerobacter subterraneus subsp. tengcongensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | Caldanaerobacter subterraneus subsp. tengcongensis | 6-oxopurine salvage pathway reaction | GMP + diphosphate | - |
r | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Caldanaerobacter subterraneus subsp. tengcongensis | 6-oxopurine salvage pathway reaction | IMP + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caldanaerobacter subterraneus subsp. tengcongensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type enzyme and His-tagged mutant L160I enzyme from Escherichia coli, wild-type enzyme to homogeneity by ion exchange and adsorption chromatography, and gel filtration, mutant in a multistep procedure, overview | Caldanaerobacter subterraneus subsp. tengcongensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Caldanaerobacter subterraneus subsp. tengcongensis | GMP + diphosphate | - |
r | |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | 6-oxopurine salvage pathway reaction | Caldanaerobacter subterraneus subsp. tengcongensis | GMP + diphosphate | - |
r | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | 6-oxopurine salvage pathway reaction | Caldanaerobacter subterraneus subsp. tengcongensis | IMP + diphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
HGPRT | - |
Caldanaerobacter subterraneus subsp. tengcongensis |
hypoxanthine-guanine phosphoribosyltransferase | - |
Caldanaerobacter subterraneus subsp. tengcongensis |