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Literature summary for 2.4.2.8 extracted from

  • You, D.; Chen, Q.; Liang, Y.; An, J.; Li, R.; Gu, X.; Luo, M.; Su, X.D.
    Protein preparation, crystallization and preliminary X-ray crystallographic studies of a thermostable hypoxanthine-guanine phosphoribosyltransferase from Thermoanaerobacter tengcongensis (2003), Acta Crystallogr. Sect. D, 59, 1863-1865.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant L160I enzymes in Escherichia coli strain BL21(DE3) Caldanaerobacter subterraneus subsp. tengcongensis

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant mutant L160I enzyme, hanging drop vapour diffusion method, 0.001 ml of 10 mg/ml protein in 20 mM Tris-HCl, pH 7.4, excess GMP in a molar ratio 4:1, is mixed with equal volume of crystallization solution containing 0.2 M CaCl2 dihydrate, 0.1 M HEPES, pH 7.5, 28% PEG 400, 20°C, 2 weeks, X-ray diffraction structure determination and analysis at 1.7 A resolution, purified recombinant wild-type enzyme, hanging drop vapour diffusion method, 0.001 ml of 10 mg/ml protein in 20 mM Tris-HCl, pH 7.4, excess IMP in a molar ratio 4:1, is mixed with equal volume of crystallization solution containing 0.2 M magnesium acetate tetrahydrate, 0.1 M sodium cacodylate, pH 6.5, 12% PEG 4000, 20°C, within 1 week, X-ray diffraction structure determination and analysis at 2.2 A resolution Caldanaerobacter subterraneus subsp. tengcongensis

Protein Variants

Protein Variants Comment Organism
L160I site-directed mutagenesis, crystal structure determination Caldanaerobacter subterraneus subsp. tengcongensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanine + 5-phospho-alpha-D-ribose 1-diphosphate Caldanaerobacter subterraneus subsp. tengcongensis 6-oxopurine salvage pathway reaction GMP + diphosphate
-
r
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Caldanaerobacter subterraneus subsp. tengcongensis 6-oxopurine salvage pathway reaction IMP + diphosphate
-
r

Organism

Organism UniProt Comment Textmining
Caldanaerobacter subterraneus subsp. tengcongensis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type enzyme and His-tagged mutant L160I enzyme from Escherichia coli, wild-type enzyme to homogeneity by ion exchange and adsorption chromatography, and gel filtration, mutant in a multistep procedure, overview Caldanaerobacter subterraneus subsp. tengcongensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Caldanaerobacter subterraneus subsp. tengcongensis GMP + diphosphate
-
r
guanine + 5-phospho-alpha-D-ribose 1-diphosphate 6-oxopurine salvage pathway reaction Caldanaerobacter subterraneus subsp. tengcongensis GMP + diphosphate
-
r
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate 6-oxopurine salvage pathway reaction Caldanaerobacter subterraneus subsp. tengcongensis IMP + diphosphate
-
r

Synonyms

Synonyms Comment Organism
HGPRT
-
Caldanaerobacter subterraneus subsp. tengcongensis
hypoxanthine-guanine phosphoribosyltransferase
-
Caldanaerobacter subterraneus subsp. tengcongensis