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Literature summary for 2.4.2.8 extracted from

  • Heroux, A.; White, E.L.; Ross, L.J.; Kuzin, A.P.; Borhani, D.W.
    Substrate deformation in a hypoxanthine-guanine phosphoribosyltransferase ternary complex: the structural basis for catalysis (2000), Structure Fold. Des., 8, 1309-1318.
    View publication on PubMed
Search for more literature for 2.4.2.8

Application

Application Comment Organism
medicine potential target for antiparasitic chemotherapy Toxoplasma gondii

Cloned(Commentary)

Cloned (Comment) Organism
-
 Toxoplasma gondii

Crystallization (Commentary)

Crystallization (Comment) Organism
the recombinant enzyme is complexed with Mg2+, 5-phosphoribosyl 1-diphosphate and inactive substrate analogue 9-deazaguanine, hanging drop method, enzyme complex, 20 mg/ml, is precipitated by 0.1 M Tris-HCl, pH 8.0, 30% polyethylene glycol 4000, 0.2 M Li2SO4, 0.5% beta-octylglucoside at 4°C, X-ray diffraction structure analysis Toxoplasma gondii

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Toxoplasma gondii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
guanine + 5-phospho-alpha-D-ribose 1-diphosphate Toxoplasma gondii
-
 GMP + diphosphate
-
 ?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Toxoplasma gondii
-
 IMP + diphosphate
-
 ?
additional information Toxoplasma gondii salvage incorporation of exogenous purine nucleotides, no de novo synthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Toxoplasma gondii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Toxoplasma gondii

Reaction

Reaction Comment Organism Reaction ID
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate catalytic mechanism Toxoplasma gondii
a
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate active site structure Toxoplasma gondii
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Toxoplasma gondii GMP + diphosphate
-
 ?
guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Toxoplasma gondii GMP + diphosphate
-
 r
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Toxoplasma gondii IMP + diphosphate
-
 ?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Toxoplasma gondii IMP + diphosphate
-
 r
additional information 9-deazaguanine is no substrate Toxoplasma gondii ?
-
 ?
additional information salvage incorporation of exogenous purine nucleotides, no de novo synthesis Toxoplasma gondii ?
-
 ?

Subunits

Subunits Comment Organism
More structure determination and analysis Toxoplasma gondii