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Literature summary for 2.4.2.8 extracted from
- Localization and targeting of the Leishmania donovani hypoxanthine-guanine phosphoribosyltransferase to the glycosome (1998), J. Biol. Chem., 273, 1534-1541.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functional expression of wild-type enzyme and mutants in an enzyme-deficient Escherichia coli strain | Leishmania donovani |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of mutant lacking the Ser-Lys-Val C-terminal targeting signal, mutant enzyme is located throughout the parasite, including subcellular organelles such as nucleus and flagellum | Leishmania donovani |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0048 | - |
hypoxanthine | mutant enzyme | Leishmania donovani |  |
| 0.0064 | - |
hypoxanthine | wild-type enzyme | Leishmania donovani | |
| 0.01 | - |
guanine | wild-type enzyme | Leishmania donovani | |
| 0.012 | - |
guanine | mutant enzyme | Leishmania donovani | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| glycosome | a fuel-metabolizing microbody unique to this parasite, exclusively | Leishmania donovani | 20015 | - |
| additional information | the mutant enzyme, lacking the targeting signal, is located throughout the parasite, including subcellular organelles such as nucleus and flagellum | Leishmania donovani | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | Leishmania donovani | - |
GMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | Leishmania donovani | - |
IMP + diphosphate | - |
? | |
| additional information | Leishmania donovani | salvage incorporation of exogenous purine nucleotides, no de novo synthesis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Leishmania donovani | - |
parasites isolated from rabbits | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant wild-type and mutant from Escherichia coli | Leishmania donovani |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| promastigote | - |
Leishmania donovani | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | GMP + diphosphate | - |
? | |
| guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | GMP + diphosphate | - |
r | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | IMP + diphosphate | - |
? | |
| hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania donovani | IMP + diphosphate | - |
r | |
| additional information | salvage incorporation of exogenous purine nucleotides, no de novo synthesis | Leishmania donovani | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.7 | - |
hypoxanthine | wild-type enzyme | Leishmania donovani | |
| 6.2 | - |
hypoxanthine | mutant enzyme | Leishmania donovani | |
| 12.1 | - |
guanine | wild-type enzyme | Leishmania donovani | |
| 15.8 | - |
guanine | mutant enzyme | Leishmania donovani | |
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