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Literature summary for 2.4.2.8 extracted from

  • Yuan, L.; Craig, S.P.; McKerrow, J.H.; Wang, C.C.
    The hypoxanthine-guanine phosphoribosyltransferase of Schistosoma mansoni. Further characterization and gene expression in Escherichia coli (1990), J. Biol. Chem., 265, 13528-13532.
    View publication on PubMed

Application

Application Comment Organism
medicine potential target for antiparasitic chemotherapy Schistosoma mansoni

Cloned(Commentary)

Cloned (Comment) Organism
DNA sequence determination, expression in Escherichia coli Schistosoma mansoni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
guanine native enzyme Schistosoma mansoni
0.0027
-
guanine native enzyme Schistosoma mansoni
0.0037
-
hypoxanthine native enzyme Schistosoma mansoni
0.0042
-
hypoxanthine recombinant enzyme Schistosoma mansoni

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Schistosoma mansoni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
26000
-
x * 26000, SDS-PAGE Schistosoma mansoni

Organism

Organism UniProt Comment Textmining
Schistosoma mansoni
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Schistosoma mansoni

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
kinetics Schistosoma mansoni
5.4
-
purified enzyme, substrate hypoxanthine Schistosoma mansoni
5.9
-
recombinant enzyme, substrate hypoxanthine Schistosoma mansoni
7.6
-
purified enzyme, substrate guanine Schistosoma mansoni
8.1
-
recombinant enzyme, substrate guanine Schistosoma mansoni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Schistosoma mansoni GMP + diphosphate
-
?
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
Schistosoma mansoni IMP + diphosphate
-
?
additional information kinetic study Schistosoma mansoni ?
-
?

Subunits

Subunits Comment Organism
? x * 26000, SDS-PAGE Schistosoma mansoni

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Schistosoma mansoni

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4
-
hypoxanthine native enzyme Schistosoma mansoni
2.6
-
hypoxanthine recombinant enzyme Schistosoma mansoni
4.3
-
guanine native enzyme Schistosoma mansoni
5
-
guanine recombinant enzyme Schistosoma mansoni

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Schistosoma mansoni