Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | overview: inhibition constant of purines and purine analogs | Escherichia coli | |
p-chloromercuribenzoate | reversed by dithiothreitol or 2-mercaptoethanol | Escherichia coli | |
purine nucleotides | and analogues, overview | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Escherichia coli | |
0.037 | - |
guanine | - |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
2 charge variant isoforms with different substrate specificities | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-amino-6-mercaptopurine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Escherichia coli | 2-amino-6-mercaptopurine ribotide + diphosphate | - |
? | |
2-hydroxy-6-mercaptopurine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Escherichia coli | 2-hydroxy-6-mercaptopurine ribotide + diphosphate | - |
? | |
6-mercaptopurine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Escherichia coli | 6-mercaptopurine ribotide + diphosphate | - |
? | |
guanine + 5-phospho-alpha-D-ribose 1-diphosphate | binds hypoxanthine 67-times less effectively than guanine and 4-times less effectively than xanthine | Escherichia coli | GMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | unlike the hypoxanthine-guanine phosphoribosyltransferase from other sources this enzyme binds hypoxanthine 67-times less effectively than guanine and 4-times less effectively than xanthine | Escherichia coli | IMP + diphosphate | - |
? | |
hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | binds hypoxanthine 67-times less effectively than guanine and 4-times less effectively than xanthine | Escherichia coli | IMP + diphosphate | - |
? | |
xanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Escherichia coli | XMP + diphosphate | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI of 4.8 for the hypoxanthine specific enzyme, pI of 5.5 for the guanine specific enzyme | Escherichia coli |
7.4 | 8.2 | - |
Escherichia coli |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | pH 7.0: about 80% of activity maximum, pH 8.5: about 70% of activity maximum | Escherichia coli |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.2 | - |
10 min, 50% loss of activity | Escherichia coli |
7 | 9.3 | 10 min, stable | Escherichia coli |
11 | - |
10 min, 50% loss of activity | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | overview, inhibition constants for diverse purine nucleotides and analogues | Escherichia coli |